Abstract
Eleven isozyme markers were utilized for quantitative trait loci (QTL) analysis in diploid potato. These markers are distributed among 7 of the 12 chromosomes and therefore give a representative, though sparse, survey of the potato genome. Tuber specific gravity and tuder dormancy were studied. Two segregating diploid populations were constructed from heterozygous self-incompatible parents. These two populations, TRP132 (127 individuals) and TRP133 (110 individuals), have a common maternal parent and combine genomes of Solanum tuberosum (haploid), S. chacoense, and S. phureja. The populations were planted at two locations in Michigan in 1990 using a randomized complete block design with three replications per location. After harvest they were characterized with the isozymes and evaluated for specific gravity and tuber dormancy. To test for QTLs, one-way analyses of variance were conducted for each locus by trait combination. Significant associations between markers and quantitative trait variation were identified, which accounted for a range from 4% to 15% of the phenotypic variation for specific gravity, and from 4.5% to 20.4% for tuber dormancy. Two-way analyses of variance between significant markers were used to identify epistatic interactions between markers. Multiple regression analyses were used to estimate the overall effect of the significant markers on the phenotypic variation for these traits. These values ranged from 15.3% and 32.3% for specific gravity. For dormancy, the significant loci accounted for 8.5% and 36.9% of the total phenotypic variation for each of the populations. We find that isozyme analysis is a useful tool for preliminary QTL studies in potato.
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Barone A, Ritter E, Schachtschabel U, Debener T, Salamini F, Gebhardt C (1990) Localization by restriction fragment length polymorphism mapping in potato of a major dominant gene conferring resistance to the potato cyst nematode Globodera rostochiensis. Mol Gen Genet 224:177–182
Beckmann J, Soller M (1983) Restriction fragment length polymorphisms in genetic improvement: methodologies, mapping and costs. Theor Appl Genet 67:35–43
Beckmann J, Soller M (1988) Detection of linkage between marker loci and loci affecting quantitative traits in crosses between segregating populations. Theor Appl Genet 76:228–236
Bernatsky R, Tanksley SD (1986) Toward a saturated linkage map of tomato based on isozymes and random cDNA sequences. Genetics 120:1095–1103
Bonierbale M, Plaisted R, Tanksley SD (1988) RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato. Genetics 120:1095–1103
Chase SA (1968) Analytical breeding in S. uberosum L. A scheme utilizing parthenotes and other diploid stocks. Can J Genet Cytol 5:359–363
Douches DS, Ludlam K (1991) Electrophoretic characterization of North American potato cultivass. Am Potato J 68:767–780
Douches DS, Quiros CF (1987) Use of 4x-2x crosses to determine gene-centromere distances of isozyme loci in Solanum species. Genome 29:519–527
Douches DS, Quiros CF (1988) Additional isozyme loci in tuberbearing Solanums: Inheritance and linkage relationships. Hered 79:377–384
Edwards MD, Stuber CW, Wendel JF (1987) Molecular-marker-facilitated investigations of quantitative-trait loci in maize. I. Numbers, genomic distribution and types of gene action. Genetics 116:113–125
Frei OM, Stuber CW, Wendel JF (1986a) Use of allozymes as genetic markers for predicting preformance in maize single cross hybrids. Crop Sci 26:37–42
Gebhardt C, Ritter E, Debener T, Schachtschabel U, Walkemeier B, Uhrig H, Salamini F (1989) RFLP analysis and linkage mapping in Solanum tuberosum. Theor Appl Genet 78:65–75
Gebhardt C, Ritter E, Barone A, Debener T, Walkemeier B, Schachtschabel U, Kaufmann H, Thompson RD, Bonierbale MW, Ganal MW, Tanksley SD, Salamini F (1991) RFLP maps of potato and their alignment with the homoeologous tomato genome. Theor Appl Genet 83:49–57
Grun P, Ochoa C, Capanage D (1977) Evolution of cytoplasmic factors in tetraploid potatoes. Am J Bot 64:412–420
Helentjaris T, King G, Slocum M, Siedenstrang C, Wegman S (1985) Restriction fragment polymorphisms as probes for plant diversity and their development as tools for applied plant breeding. Plant Mol Biol 5:109–118
Helentjaris T, Slocum M, Wright S, Schaefer A, Nienhuis J (1986) Construction of genetic linkage maps in maize and tomato using restriction fragment length polymorphisms. Genetics 118:353–363
Iwanaga M (1983) Ploidy level manipulation approach: development of diploid populations with specific resistance and FDR 2n pollen production. In: Present and future strategies for potato breeding and improvement. Report 26th Planning Conf. CIP. Lima, Peru, pp 57–70
Kahler AL, Wehrhahn CF (1986) Associations between quantitative traits and enzyme loci in the F2 population of a maize hybrid. Theor Appl Genet 72:15–26
Landeo J (1979) Breeding potential of Group Andigena haploid potatoes. PhD thesis, University of Wisconsin, Madison
Lander E, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199
Landry BS, Kesseli RV, Farrara B, Michelmore RW (1987) A genetic map of lettuce (Lactuca sativa L.) with restriction fragment length polymorphism, isozyme, disease resistance and morphological markers. Genetics 116:331–337
McCouch SR, Kochert G, Yu ZH, Wang ZY, Kush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829
Mendoza H, Haynes F (1974) Genetic relationships among potato cultivars grown in the United States. Hortscience 9:328–330
Nienhuis J, Helentjaris T, Slocum M, Ruggero B, Schaefer A (1987) Restriction fragment length polymorphism analysis of loci associated with insect resistance in tomato. Crop Sci 27:797–803
Owings T (1979) Cultural practices which influence the specific gravity of ‘Russet Burbank’. In: Proc. 18th Annu Washington Potato Conf Trade Fair, pp 41–47
Paterson AH, Damon S, Hewitt JD, Zamir D, Rabinowitch HD, Lincoln SE, Lander ES, Tanksley SD (1991) Mendelian factors underlying quantitative traits in tomato: comparison across species, generations and environments. Genetics 127:181–197
Peloquin SJ, Yerk GL, Werner JE, Darmo E (1989) Potato breeding with haploids and 2n gametes. Genome 31:1000–1004
Pollack LM, Gardner CO, Parkhurst AM (1984) Relationships between enzyme marker loci and morphological traits in two mass selected maize populations. Crop Sci 24:1174–1179
Ritter E, Debener T, Barone A, Salamini F, Gebhardt C (1991) RFLP mapping on potato chromosomes of two genes conferring resistance to potato virus X (PVX). Mol Gen Genet 227:81–85
Ruttencutter G, Haynes F, Moll R (1979) Estimation of narrowsense heritability for specific gravity in diploid potatoes (S. tuberosum subsp. phureja and stenotomum) Am Potato J 56:447–453
Sax K (1923) The association of size differences with seed coat pattern and pigmentation in Phaseolus vulgaris. Genetics 120:597–604
Slocum MK, Figdore SS, Kennard WC, Suzuki JY, Osborn TC (1990) Linkage arrangement of restriction fragment length polymorphism in Brassica oleracea. Theor Appl Genet 80:57–67
Stuber CW (1989) Comparative studies using RFLPs and isozymes as molecular markers for the study and analyses of multigenic traits in maize. In: Helentjaris T, Burr B (eds) Development and application of molecular markers to problems in plant genetics Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., pp 103–106
Stuber CW (1989) Isozymes as markers for studying and manipulating quantitative trials. In: Soltis DE, Soltis PS (eds) Isozynes in plant biology. Dioscorides Press, pp 206–220
Tanksley SD (1983) Molecular markers in plant breeding. Plant Mol Biol Rep 1:3–8
Tanksley SD, Rick CM (1980) Isozyme linkage map of the tomato: applications in genetics and breeding. Theor Appl Genet 57:161–170
Tanksley SD, Medina-Filho H, Rick CM (1982) Use of naturally occurring enzyme variation to detect and map genes controlling quantitative traits in an interspecific backcross of tomato. Heredity 49:11–25
Tanksley SD, Bernatzky R, Lapitan NL, Prince JP (1988) Conservation of gene repertoire but not gene order in pepper and tomato. Proc Natl Acad Sci USA 85:6419–6423
Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: new tools for an old science. Biotechnology 7:257–264
Thompson P, Haynes F, Moll R (1980) Estimation of genetic variance components and heritability for tuber dormancy in diploid potatoes. Am Potato J 57:39–46
Weller JI (1987) Mapping and analysis of quantitative trait loci in Lycopersicon (tomato) with the aid of genetic markers using approximate maximum likelihood methods. Heredity 59:413–421
Weller JI, Soller M, Brody T (1988) Linkage analysis of quantitative traits in an interspecific cross of tomato (Lycopersicon esculentum x Lycopersicon pimpinellifolium) by means of genetic markers. Genetics 118:329–339
Wilson JH, Lindsay AM (1959) The relationship between specific gravity and dry matter content of potato tubers. Am Potato J 46:323–328
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Communicated by A. R. Hallauer
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Freyre, R., Douches, D.S. Isoenzymatic identification of quantitative traits in crosses between heterozygous parents: mapping tuber traits in diploid potato (Solanum spp.). Theoret. Appl. Genetics 87, 764–772 (1994). https://doi.org/10.1007/BF00221127
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DOI: https://doi.org/10.1007/BF00221127