Summary
In the testis of Esox lucius at the time of spermiation, activity of cyclic adenosine 3′,5′-monophosphate (cAMP) was immunocytochemically localized at the level of the Sertoli cells. In these cells adenylate cyclase activity was also ultracytochemically demonstrated by using adenylyl imidodiphosphate as a substrate. Reaction products of adenylate cyclase were primarily detectable on the basal and adluminal plasma membranes and on the surface of protrusions of the cell body into the lumen.
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Abraham M, Rehamim E, Tibika H (1979) The blood-testis barrier in teleosts. Abstr I Congr Eur Comp Endocrinol Soc (Sorrento)
Abraham M, Rehamim E, Tibika H, Golenser E (1980) The bloodtestis barrier in Aphanius dispar (Teleostei). Cell Tissue Res 211:207–214
Billard R (1983) A quantitative analysis of spermatogenesis in the trout Salmo trutta fario. Cell Tissue Res 230:495–502
Billard R, Breton B, Jalambert B (1971) La production spérmatogénique chez la truite. Ann Biol Anim Biochem Biophys 11:190–212
Billard R, Fostier A, Weil C, Breton B (1982) Endocrine control of spermatogenesis in teleost fish. Can J Fish Aquat Sci 39:65–79
Clemens HP, Grant FB (1965) The seminal thinning response of carp (Cyprinus carpio) and rainbow trout (Salmo gairdnerii) after injections of pituitary extracts. Copeia 2:174–177
Clemens HP, Ciereszko LS, Shoemaker JD, Grant FB (1964) Partial characterization of the gonadal hydration principle in the pituitaries of carp (Cyprinus carpio). Gen Comp Endocrinol 4:503–507
Cutler LS (1983) Cytochemical methods for the localization of adenylate cyclase. J Histochem Cytochem 31:85–93
Davoren PR, Sutherland EW (1963) Cellular localization of adenyl cyclase in the pigeon erythrocyte. J Biol Chem 238:3016–3023
Dorrington JH, Vernon RG, Fritz IB (1972) The effect of gonadotropins on the 3′,5′-cyclic AMP levels of seminiferous tubules. Biochem Biophys Res Commun 46:1523–1528
Grier HJ, Linton JR, Leatherland F, De Vlaming VL (1980) Structural evidence for two different testicular types in teleost fishes. Am J Anat 159:331–345
Howell SL, Whitfield M (1972) Cytochemical localization of adenylate cyclase activity in rat islets of Langherhans. J Histochem Cytochem 20:873–879
Idler DR, Ng TB (1979) Studies on two types of gonadotropins from both salmon and carp pituitaries. Gen Comp Endocrinol 38:421–440
Marcaillou C, Szollosi A (1980) The blood-testis barrier in a nematode and a fish: a generalizable concept. J Ultrastruct Res 70:128–136
Morisawa M, Okuno M (1982) Cyclic AMP induces maturation of trout sperm axoneme to initiate sperm motility. Nature 295:703–704
Morisawa M, Okuno M, Suzuki K, Morisawa S, Ishida K (1983) Initiation of sperm motility in teleosts. Submicrose Cytol 15:61–65
Ng TB, Idler DR (1980) Gonadotropic regulation of androgen production in flounder and salmonids. Gen Comp Endocrinol 42:25–38
Pascolini R, Vagnetti D, Spreca A, Marinelli M, Lorvik S (1983) Cytochemical study on the distribution of adenylate cyclase in guinea pig testis. Anat Rec 207:4 (in press)
Rall TW, Sutherland EW (1962) Adenyl cyclase II: Enzymatically catalyzed formation of adenosine 3′,5′-phosphate and inorganic pyrophosphate from adenosine triphosphate. J Biol Chem 237:1228–1235
Sternberger LA (1979) The unlabeled antibody peroxidase-antiperoxidase (PAP) method. In: Immunocytochemistry. John Wiley & Sons eds, New York, pp 104–169
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Pascolini, R., Ceccarelli, P., Gargiulo, A.M. et al. Immunohistochemical localization of cyclic AMP and ultrastructural demonstration of adenylate cyclase activity in the testis of Esox lucius at time of spermiation. Cell Tissue Res. 239, 443–445 (1985). https://doi.org/10.1007/BF00218025
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DOI: https://doi.org/10.1007/BF00218025