Summary
Single and double immunocytochemical techniques were applied to the brain and pituitary of carps and goldfish. With the use of antiserum raised against synthetic corticotropin-releasing factor (CRF), immunoreactive perikarya were observed in the nucleus praeopticus and the nucleus praeopticus periventricularis. CRF-like-immunoreactive hypothalamic nerve fibers reach the pituitary. In cyprinids, some fine fibers enter the rostral neurohypophysis bordered by prolactin- and ACTH cells. Other thicker fibers extend ventrocaudally into the neurointermediate lobe. This CRF-like system appears to differ from the SRIF-like system, which is restricted to the proximal pars distalis of the pituitary containing somatotrophs.
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The technical assistance of Jacqueline Olivereau, Biologiste-Adjointe from the CNRS, is gratefully acknowledged
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Olivereau, M., Ollevier, F., Vandesande, F. et al. Immunocytochemical identification of CRF-like and SRIF-like peptides in the brain and the pituitary of cyprinid fish. Cell Tissue Res. 237, 379–382 (1984). https://doi.org/10.1007/BF00217162
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DOI: https://doi.org/10.1007/BF00217162