Summary
In the rabbit, the pseudopregnant uterus has been used as a model for studying alterations characteristic of the preimplantation phase. Alterations in intercellular junctions of the uterine epithelium were investigated during early pseudopregnancy (day 0 to day 6) by means of the freeze-fracture technique.
In the uterine epithelium of oestrous females the zonula occludens belongs to the “tight” type of tight junctions. During pseudopregnancy an impressive proliferation of tight junctional belts can be observed. The basal strands proliferate, forming loops perpendicular to the luminal surface, whereas the more or less parallel arrangement of the luminal strands is maintained. At day 4 of pseudopregnancy macular tight junctions begin to develop on the lower portions of the lateral plasmalemma and are extensive by day 6 post hCG.
Small gap junctions are infrequent between cells of the uterine epithelium and show no significant changes during the preimplantation phase.
The physiological significance of the present morphological observations is discussed in the light of changes occurring during the preimplantation period.
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References
Albertini DF, Fawcett DW, Olds PJ (1975) Morphological variations in gap junctions of ovarian granulosa cells. Tissue Cell 7:389–405
Beier HM (1980) The role of uterine proteins in the establishment of receptivity of the uterus. Prog Reprod Biol 7:158–192
Beier HM, Kühnel W (1973) Pseudopregnancy in the rabbit after stimulation by human chorionic gonadotropin. Horm Res 4:1–27
Beier HM, Kühnel W (1976) Untersuchungen zur funktionellen Morphologie des Epithels der Endosalpinx und des Endometriums. Verh Anat Ges 70:831–838
Burghardt RC, Anderson E (1981) Hormonal modulation of gap junctions in rat ovarian follicles. Cell Tissue Res 214:181–193
Busch LC, Kühnel W, Mootz U (1981) Scanning electron microscopical studies of the rabbit endometrium during estrus and preimplantation. In: DiDio SJA, Motta PM, Allen DJ (eds) Three dimensional microanatomy of cells and tissue surfaces. Elsevier, North Holland Inc
Busch LC, Winterhager E, Kühnel W (1981) Symplasmatische Umwandlung des Cavumepithels im Uterus pseudogravider Kaninchen. Acta Anat 111:22
Claude P (1978) Morphological factors influencing transepithelial permeability. A model for the resistance of the zonula occludens. J Membr Biol 39:219–232
Claude P, Goodenough DA (1973) Fracture faces of zonulae occludentes from “tight” and “leaky” epithelia. J Cell Biol 58:390–400
Dahl G, Berger W (1978) Nexus formation in the myometrium during parturition and induced by estrogen. Cell Biol Int Rep 2:377–381
Davies J, Hoffman LH (1973) Studies on the progestational endometrium of the rabbit. I. Light microscopy, day 0 to day 13 of gonadotrophin induced pseudopregnancy. Am J Anat 137:423–446
Davies J, Hoffman LH (1975) Studies on the progestational endometrium of the rabbit. II. Electron microscopy, day 0 to day 13 of gonadotrophin induced pseudopregnancy. Am J Anat 142:335–366
Decker RS (1976) Hormonal regulation of gap junctions differentiation. J Cell Biol 69:669–685
Decker RS, Friend DS (1974) Assembly of gap junctions during amphibian neurulation. J Cell Biol 62:32–47
Elias PM, Friend DS (1976) Vitamin-A induced mucous metaplasia. An in vitro system for modulating tight and gap junction differentiation. J Cell Biol 68:173–188
Friend DS, Gilula NB (1972) Variations in tight and gap junctions in mammalian tissues. J Cell Biol 53:758–776
Garfield RE, Kannan WS, Daniel EE (1980) Gap junction formation in myometrium: control by estrogens, progesterone, and prostaglandins. Am J Physiol 239:C217-C228
Greven H, Robenek H (1980) Intercellular junctions in the uterine epithelium of Salamandra salamandra (L.) (Amphibia, Urodela). A freeze-fracture study. Cell Tissue Res 212:163–172
Hull BE, Staehelin FR (1976) Functional significance of the variations in the geometrical organization of tight junction network. J Cell Biol 68:688–704
Kühnel W, Busch LC (1981) Functional morphology of the oviductal mucosa and the endometrium as viewed by SEM. Biomed Res 2:341–353
Luciano L, Thiele J, Reale E (1979) Development of follicles and of occluding junctions between the follicular cells of thyroid gland. J Ultrastruct Res 66:164–181
Merk FB, Botticelli ChR, Albright JT (1972) An intercellular response to estrogen by granulosa cells in the rat ovary: An electron microscope study. Endocrinology 90:992–1007
Meza J, Ibarra G, Sabanero M, Martinez-Palomo A, Cereijido M (1980) Occluding junctions and cytoskeletal components in a cultured transporting epithelium. J Cell Biol 87:746–754
Montesano R, Friend DS, Perrelet A, Orci L (1975) In vivo assembly of tight junctions in foetal rat liver. J Cell Biol 67:310–319
Murphy CR, Rogers AW (1980) Ovarian hormones alter tight junction structure in uterine luminal epithelial cells. Micron 11:375–376
Pitelka DR, Hammamoto ST, Duafala JG, Nemamic MK (1973) Cell contacts in the mouse mammarian gland. I. Normal gland in postnatal development and secretory cycle. J Cell Biol 56:797–818
Rassat J, Robenek H, Themann H (1981) Ultrastructural changes in mouse hepatocytes exposed to vinblastine sulfate with special reference to intercellular junctions. Europ J Cell Biol 24:203–210
Robenek H, Meiss R, Gehling J, Themann H (1980a) Alterations in the rat liver induced by P-chlor-M-cresol with emphasis on the intercellular junctions. J Submicrosc Cytol 12:635–646
Robenek H, Döldissen M, Themann H (1980b) Morphological changes of tight junctions in the rat liver after chronic administration of N-nitrosomorpholine (NNM) as revealed by freeze-fracturing. J Ultrastruct Res 70:82–91
Robenek H, Herwig J, Themann H (1980c) The morphologic characteristic of intercellular junctions between normal human liver cells and cells from patients with extrahepatic cholestasis. Am J Pathol 100:93–114
Schiller A, Taugner R (1979) Junctions between interstitial cells of the renal medulla: a freeze-fracture study. Cell Tissue Res 203:231–240
Suzuki H, Tsutsumi Y (1980) Morphological changes of uterine and cervical epithelium during early pregnancy in rabbits. J Fac Agr Hokkaido Univ 60:Pt 1
Suzuki H, Tsutsumi Y (1980) Morphological studies of uterine and cervical epithelium in pseudopregnant rabbits. J Fac Agr Hokkaido Univ 60:Pt2
Tice LW, Wollmann SH, Carter RC (1975) Changes in tight junctions of thyroid epithelium with changes in thyroid activity. J Cell Biol 66:657–663
Weinstein RS, Merk FB, Alroy J (1976) The structure and function of intercellular junctions in cancer. Adv Cancer Res 23:23–90
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Winterhager, E., Kühnel, W. Alterations in intercellular junctions of the uterine epithelium during the preimplantation phase in the rabbit. Cell Tissue Res. 224, 517–526 (1982). https://doi.org/10.1007/BF00213749
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DOI: https://doi.org/10.1007/BF00213749