Abstract
The expression of hsp 70 after heat shock or exposure to heavy metals/molluscicides was investigated by fluorography or immunoblot in three diplopods (Glomeris marginata, Cylindroiulus punctatus, Tachypodoiulus niger), two slugs (Deroceras reticulatum, Arion ater), and one isopod (Oniscus asellus). In O. asellus, hsp 70 expression occurred after heat shock and also after lead treatment, whereby a solution of 100 mg/kg Pb2+ was sufficient. Animals of the same species taken from a heavy metal polluted site in the vicinity of a lead/zinc smelter also showed the presence of hsp 70. The comparision of laboratory and field experiments demonstrated the suitability of O. asellus for monitoring tests. In contrast, the blot pattern after contamination with 1,000 mg/kg Pb2+ (in the mentioned diplopods) or different concentrations of the molluscicide Cloethocarb (BASF, FRG) (slugs) showed no differences compared to the respective control group.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Anderson NL, Giometti CS, Gemmell MA, Nance SL, Anderson NG (1982) A two-dimensional electrophoretic analysis of the heat-shock-induced proteins of human cells. Clin Chem 28:1084–1092
Ashburner M, Bonner JJ (1979) The induction of gene activity in Drosophila by heat shock. Cell 17:241–254
Craig E, Ingolia T, Slater M, Manseau L (1982) Drosophila and yeast multigene families related to the Drosophila heat shock genes. In: Schlesinger MJ, Ashburner M, Tissières A (eds) Heat shock: From bacteria to man. Cold Spring Harbor Lab, Cold Spring Harbor, NY, pp 11–18
Craig EA, Ingolia TD, Manseau LJ (1983) Expression of Drosophila heat shock cognate genes during heat shock and development. Dev Biol 99:418–426
Currie RW, White FP (1981) Trauma induced protein in rat tissues: a physiological role for a “heat shock” protein? Science 214:72–73
Dallinger R, Prosi F (1988) Heavy metals in the terrestrial isopod Porcellio scaber Latreille. II. Subcellular fraction of metal accumulating lysosomes from hepatopancreas. Cell Biol Toxicol 4:97–109
Garry RF, Ulug ET, Bose HR (1983) Induction of stress proteins in Sindbis virus- and vesicular stomatitis virus-infected cells. Virology 129:319–332
Heikkila JJ, Schultz GA, Iatrou K, Gedamu L (1982) Expression of a set of fish genes following heat or metal ion exposure. J Biol Chem 257:12000–12005
Hightower LE, White FP (1982) Preferential synthesis of rat heat shock and glucose-regulated protein in stressed cardiovascular cells. In: Schlesinger MJ, Ashburner M, Tissières A (eds) Heat shock: From bacteria to man. Cold Spring Harbor Lab, Cold Spring Harbor, NY, pp 369–377
Ireland RC, Berger EM (1982) Synthesis of low molecular weight heat shock peptides stimulated by ecdysterone in a cultured Drosophila cell line. Proc Natl Acad Sci USA 79:855–859
Kelley PM, Schlesinger MJ (1982) Antibodies to two major chicken heat shock proteins cross-react with similar proteins in widely divergent species. Mol Cell Biol 2:267–274
Köhler H-R, Alberti G (in press) The effect of heavy metal stress on the intestine of diplopods. Proc 8th Int Congr Myriapodology, Innsbruck, 1990
Levinson W, Idriss J, Jackson J (1979) Metal-binding drugs induce synthesis of four proteins in normal cells. Biol Trace Element Res 1:15–23
Levinson W, Oppermann H, Jackson, J. (1980) Transition series metals and sulfhydryl reagents induce the synthesis of four proteins in eucaryotic cells. Biochim Biophys Acta 606:170–180
Nover L (1984) Heat shock response of eucaryotic cells. Springer, Berlin, Heidelberg, New York, Tokyo
Shelton KR, Todd JM, Egle PM (1986) The induction of stress-related proteins by lead. J Biol Chem 261:1935–1940
Stöcker W, Raeder U, Bijlholt MMC, Wichertjes T, van Bruggen EFJ, Markl J (1988) The quaternary structure of four crustacean two-hexameric hemocyanins: immunocorrelation, stoichiometry, reassembly and topology of individual subunits. J Comp Physiol B 158:271–289
Tissières A, Mitchell HK, Tracy UM (1974) Protein synthesis in salivary glands of D. melanogaster. Relation to chromosome puffs. J Mol Biol 84:389–398
Voellmy R, Bromley PA (1982) Massive heat-shock polypeptide synthesis in late chicken embryos: convenient system for study of protein synthesis in highly differentiated organisms. Mol Cell Biol 2:479–483
Winter J, Wright R, Duck N, Gasser C, Fraley R, Shah D (1988) The inhibition of petunia hsp 70 mRNA processing during CdCl2 stress. Mol Gen Genet 211:315–319
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Köhler, HR., Triebskorn, R., Stöcker, W. et al. The 70 kD heat shock protein (hsp 70) in soil invertebrates: A possible tool for monitoring environmental toxicants. Arch. Environ. Contam. Toxicol. 22, 334–338 (1992). https://doi.org/10.1007/BF00212095
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00212095