Abstract
Whole-canopy measurements of water flux were used to calculate stomatal conductance (g s ) and transpiration (E) for seedlings of western water birch (Betula occidentalis Hook.) under various soil-plant hydraulic conductances (k), evaporative driving forces (ΔN; difference in leaf-to-air molar fraction of water vapor), and soil water potentials (Ψs). As expected, g s dropped in response to decreased k or ΨS, or increased ΔN(> 0.025). Field data showed a decrease in mid-day g s with decreasing k from soil-to-petiole, with sapling and adult plants having lower values of both parameters than juveniles. Stomatal closure prevented E and Ψ from inducing xylem cavitation except during extreme soil drought when cavitation occurred in the main stem and probably roots as well. Although all decreases in g s were associated with approximately constant bulk leaf water potential (ψl), this does not logically exclude a feedback response between ΨL and g s . To test the influence of leaf versus root water status on g s , we manipulated water status of the leaf independently of the root by using a pressure chamber enclosing the seedling root system; pressurizing the chamber alters cell turgor and volume only in the shoot cells outside the chamber. Stomatal closure in response to increased ΔN, decreased k, and decreased ΨS was fully or partially reversed within 5 min of pressurizing the soil. Bulk ΨL remained constant before and after soil pressurizing because of the increase in E associated with stomatal opening. When ΔN was low (i.e., < 0.025), pressurizing the soil either had no effect on g s , or caused it to decline; and bulk ΨL increased. Increased Ψl may have caused stomatal closure via increased backpressure on the stomatal apparatus from elevated epidermal turgor. The stomatal response to soil pressurizing indicated a central role of leaf cells in sensing water stress caused by high ΔN, low k, and low ΨS. Invoking a prominent role for feedforward signalling in short-term stomatal control may be premature.
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Abbreviations
- ABA:
-
abscisic acid
- E :
-
transpiration rate
- g b :
-
boundary-layer conductance to water vapor
- g s :
-
stomatal conductance to water vapor
- g t :
-
total conductance to water vapor
- k :
-
leaf-specific hydraulic conductance, subscripts
- S-T :
-
soil to trunk
- T-P :
-
trunk to petiole
- T-L :
-
trunk to lamina
- S-P :
-
soil to petiole
- S-L :
-
soil to lamina
- ΔN :
-
difference in molar fraction of water vapor inside the leaf and ambient air
- Ψ:
-
water potential, subscripts
- S:
-
soil
- T:
-
trunk
- L:
-
leaf
References
Chazen, O., Newmann, P.M. (1994) Hydraulic signals from the roots and rapid cell-wall hardening in growing maize (Zea mays L.) leaves are primary responses to polyethylene glycol-induced water deficits. Plant Physiol. 104, 1385–1392
Comstock, J., Ehleringer, J. (1993) Stomatal response to humidity in common bean (Phaseolus vulgaris): implications for maximum transpiration rate, water-use efficiency and productivity. Aust. J. Plant Physiol. 20, 669–691
Davies, W.J., Zhang, J. (1991) Root signals and the regulation of growth and development of plants in drying soil. Annu. Rev. Plant Physiol. Plant Mol. Biol. 42, 55–76
Donovan, L.A., Ehleringer, J.R. (1992) Contrasting water-use patterns among size and life-history classes of a semi-arid shrub. Funct. Ecol. 6, 482–488
Farquhar, G.D. (1978) Feedforward responses of stomata to humidity. Aust. J. Plant Physiol. 5, 787–800
Fuchs, E., Livingston, N. (1994) Hydraulic control of stomatal conductance in douglas fir (Psuedotsuga menziesii) and alder (Alnus oregona) in drying soils. (Abstr.) Plant Physiol. 105, Suppl., 14
Gollan, T., Passioura, J.B., Munns, R. (1986) Soil water status affects the stomatal conductance of fully turgid wheat and sunflower leaves. Aust. J. Plant Physiol. 13, 459–464
Grantz, D.G. (1990) Plant response to atmospheric humidity. Plant Cell Environ. 13, 667–679
Janes, B.E., Gee, G.W. (1973) Changes in transpiration, net carbon dioxide assimilation and leaf water potential resulting from application of hydrostatic pressure to roots of intact pepper plants. Physiol. Plant. 28, 201–208
Jones, H.G. (1990) Physiological aspects of the control of water status in horticultural crops. HortSci. 25, 19–26
Jones, H.G., Sutherland, R.A. (1991) Stomatal control of xylem embolism. Plant Cell Environ. 14, 607–612
Ludlow, M.M. (1980) Adaptive significance of stomatal responses to water stress. In: Adaptation of plants to water and high temperature stress, pp. 123–138, Turner, N.C., Kramer, P.J., eds. John Wiley and Sons, New York
Malone, M. (1993) Hydraulic signals. Philos. Trans. R. Soc. London Ser on B 341, 33–39
Markhart, A.H., Smit, B. (1990) Measurement of root hydraulic conductance. HortSci. 25, 282–287
McDermitt, D.K. (1990) Sources of error in the estimation of stomatal conductance and transpiration from porometer data. HortSci. 25, 1538–1548
Meinzer, F.C. (1993) Stomatal control of transpiration. Tree 8, 289–294
Meinzer, F.C., Grantz, D.G. (1990) Stomatal and hydraulic conductance in growing sugarcane: stomatal adjustment to water transport capacity. Plant Cell Environ. 13, 383–388
Meinzer, F.C., Goldstein, G., Neufeld, H.S., Grantz, D.A., Crisosto, G.M. (1992) Hydraulic architecture of sugarcane in relation to patterns of water use during plant development. Plant Cell Environ. 15, 471–477
Mott, K.A., Cardon, Z.G., Berry, J.A. (1993) Asymmetric patchy stomatal closure for the two surfaces of Xanthium strumarium L. leaves at low humidity. Plant Cell Environ. 16, 25–34
Nonami, H., Schulze, E.D., Zeigler, H. (1990) Mechanisms of stomatal movement in response to air humidity, irradiance and xylem water potential. Planta 183, 57–64
Passioura, J.B., Munns, R. (1984) Hydraulic resistance of plants. II. Effects of rooting medium, and time of day, in barley and lupin. Aust. J. Plant Physiol. 11, 341–350
Pierce, M., Raschke, K. (1980) Correlation between loss of turgor and accumulation of abscisic acid in detached leaves. Planta 148, 341–350
Raschke, K. (1970) Stomatal responses to pressure changes and interruptions in the water supply of detached leaves of Zea mays L. Plant Physiol. 45, 415–423
Raschke, K. (1987) Action of Abscisic acid on guard cells. In: Stomatal function, pp. 253–280, Zeiger, E., Farquhar, G.D., Cowan, I.R., eds. Stanford University Press, Stanford, California, USA
Schulze, E.D. (1991) Water and nutrient interactions with plant water stress. In: Response of plants to multiple stresses, Mooney, H.A., Winner, W.E., Pell, E.J., eds. Academic Press, New York
Schulze, E.D. (1993) Soil water deficits and atmospheric humidity as environmental signals, In: Water deficits; plant responses from cell to community, pp. 129–146, Smith, J.A.C., Griffiths, H., eds. Bios Scientific Publishers LTD, Oxford, UK
Schurr, U., Gollan, T., Schulze, E.D. (1992) Stomatal response to drying soil in relation to changes in the xylem sap composition of Helianthus annuus. II. Stomatal sensitivity to abscisic acid imported from the xylem sap. Plant Cell Environ. 15, 561–567
Shackel, K.A., Brinckmann, E. (1985) In situ measurement of epidermal cell turgor, leaf water potential and gas exchange in Tradescantia virginiana L. Plant Physiol. 78, 66–70
Sperry, J.S., Alder, N.N., Eastlack, S.E. (1993) The effect of reduced hydraulic conductance on stomatal conductance and xylem cavitation. J. Exp. Bot. 44, 1075–1082
Sperry, J.S., Donnelly, J.R., Tyree, M.T. (1988) A method for measuring hydraulic conductivity and embolism in xylem. Plant Cell Environ. 11, 35–40
Sperry, J.S., Saliendra, N.Z. (1994) Intra- and inter-plant variation in xylem cavitation in Betula occidentalis. Plant Cell Environ. 17, 1233–1241
Tardieu, F. (1993) Will increases in our understanding of soil-root relations and root signalling substantially alter water flux models? Philos. Trans. R. Soc. London Ser. B 341, 57–66
Tardieu, F., Davies, W.J. (1993) Integration of hydraulic and chemical signalling in the control of stomatal conductance and water status of droughted plants. Plant Cell Environ. 16, 341–349
Teskey, R.O., Hinckley, T.M., Grier, C.C. (1983) Effect of interruption of flow path on stomatal conductance of Abies amabilis. J. Exp. Bot. 34, 1251–1259
Turner, N.C. (1974) Stomatal response to light and water under field conditions. In: Mechanisms of regulation of plant growth, Bieleski, R.L., Ferguson, A.R, Cresswell, M.M., eds. R. Soc. NZ Bull. 12, 423–432
Turner, N.C. (1986) Crop water deficits: a decade of progress. Adv. Agron. 39, 1–51
Tyree, M.T., Sperry, J.S. (1988) Do woody plants operate near the point of catastrophic xylem dysfunction caused by dynamic water stress? Answers from a model. Plant Physiol. 88, 574–580
Von Caemmerer, S., Farquhar, G.D. (1981) Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves. Planta 153, 376–387
Zimmermann, M.H. (1983) Xylem structure and the ascent of sap. Springer, Berlin
Zhang, J., Davies, W.J. (1991) Antitranspirant activity in xylem sap of maize. J. Exp. Bot. 42, 317–321
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We acknowledge the funding provided by the United States Department of Agriculture (grant 9202487). We thank Nathan Alder for performing portions of the embolism measurements. The comments given by two anonymous reviewers to improve the manuscript are appreciated.
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Saliendra, N.Z., Sperry, J.S. & Comstock, J.P. Influence of leaf water status on stomatal response to humidity, hydraulic conductance, and soil drought in Betula occidentalis . Planta 196, 357–366 (1995). https://doi.org/10.1007/BF00201396
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DOI: https://doi.org/10.1007/BF00201396