Abstract
Interneurons with dendritic branches in the antennal lobe of the male turnip moth, Agrotis segetum (Schiff., Lepidoptera: Noctuidae), were investigated with intracellular recording and staining methods. Seventeen projection neurons that transmit information from the antennal lobe to higher centers in the brain displayed dendritic arbors in the male specific macroglomerular complex (MGC) and responded to chemical components of the female sex pheromone used in species-specific sexual communication. Most of the projection neurons responded to several of the pheromone components tested, and a precise correlation between the location of the dendritic arborization and the physiological response could not be demonstrated. One MGC-projection neuron fit the definition of “blend specialist”. It did not respond to the individual components of the behaviorally active pheromone blend, but showed a strong response to the components when combined in the species-specific blend. Some of the projection neurons also showed clear responses to phenylacetaldehyde, a flower-produced compound and/or to (E)-2-hexenal, a common green-leaf volatile. In eight neurons, the axonal projection could be followed to the calyces of the mushroom body, and subsequently to the inferior lateral protocerebrum.
Four local interneurons were characterized both morphologically and physiologically. Each neuron arborized extensively throughout the antennal lobe, and each responded to one or several of the pheromone compounds, and/or to one or both of the plant-produced compounds. One of the local interneurons responded exclusively to the pheromone blend, but not to the individual components.
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Abbreviations
- AL :
-
antennal lobe
- AN :
-
antennal nerve
- CB :
-
cell body
- E2H :
-
(E)-2-hexenal
- IACT :
-
inner antennocerebral tract
- ILPR :
-
inferior lateral protocerebrum
- LH :
-
lateral horn of the protocerebrum
- LN :
-
local interneuron
- MB :
-
mushroom body
- MGC :
-
macroglomerular complex
- OACT :
-
outer antennocerebral tract
- PAA :
-
phenylacetaldehyde
- PN :
-
projection interneuron
- RN :
-
receptor neuron
- Z5-10:OAc :
-
(Z)-5-decenyl acetate
- Z5-10:OH :
-
(Z)-5-decenol
- Z5-12:OAc :
-
(Z)-5-dodecenyl acetate
- Z7-12:OAc :
-
(Z)-7-dodecenyl acetate
- Z9-14:OAc :
-
(Z)-9-tetradecenyl acetate
References
Arn H, Städler S, Rauscher S, Buser HR, Mustaparta H, Esbjerg P (1980) Multicomonent sex pheromone in Agrotis segetum: preliminary analysis and field evaluation. Z Naturforsch 35c:986–989
Boeckh J, Boeckh V (1979) Threshold and odor specificity of pheromone-sensitive neurons in the deutocerebrum of Antheraea pernyi and A. polyphemus (Saturnidae). J Comp Physiol 132:235–242
Boeckh J, Tolbert LP (1993) Synaptic organization and development of the antennal lobe in insects. Microscs Res Tech 24:260–280
Bretschneider F (1924) Über die Gehirne des Eichenspinners und des Scidenspinners (Lasiocampa querqus L. und Bombyx mori L.). Jena Z Naturw (Zool) 60:563–570
Christensen TA, Hildebrand JG (1987) Male-specific, sex pheromone-selective projection neurons in the antennal lobes of the moth Manduca sexta. J Comp Physiol A 160:553–569
Christensen TA, Mustaparta H, Hildebrand JG (1989) Discrimination of sex pheromone blends in the olfactory system of the moth. Chem Senses 14:463–477
Christensen TA, Mustaparta H, Hildebrand JG (1991) Chemical communication in heliothine moths II. Central processing of intraspecific and interspecific olfactory messages in the male corn earworm moth Helicoverpa zea. J Comp Physiol A 169:259–274
Christensen TA, Waldrop B, Harrow ID, Hildebrand JG (1993) Local interneurons and information processing in the olfactory glomeruli of the moth Manduca sexta. J Comp Physiol A 173:385–399
Hallberg E (1981) Fine-structural characteristics of the antennal sensilla of Agrotis segetum (Insecta: Lepidoptera). Cell Tissue Res 218:209–218
Hansson BS, Löfstedt C, Roelofs WL (1987) Inheritance of olfactory response to sex pheromone components in Ostrinia nubilalis. Naturwissenschaften 74:497–499
Hansson BS, Van der Pers JNC, Löfqvist J (1989) Comparison of male and female olfactory cell response to pheromone compounds and plant volatiles in the turnip moth, Agrotis segetum. Physiol Entomol 14:147–155
Hansson BS, Tòth M, Löfstedt C, Szöcs G, Subchev M, Löfqvist J (1990) Pheromone variation among eastern European and a western Asian population of the turnip moth Agrotis segetum. J Chem Ecol 16:1611–1622
Hansson BS, Christensen TA, Hildebrand JG (1991) Functionally distinct subdivisions of the macroglomerular complex in the antennal lobe of the male sphinx moth Manduca sexta. J Comp Neurol 312:264–278
Hansson BS, Ljungberg H, Hallberg E, Löfstedt C (1992) Functional specialization of olfactory glomeruli in a moth. Science 256:1313–1315
Haynes KF, Zhao JZ, Latif A (1991) Identification of floral compounds from Abelia grnadiflora that stimulate upwind flight in cabbage looper moths. J Chem Ecol 17:637–646
Hinks CF, Byers JR (1976) Biosystematics of the genus Euxoa (Lepidoptera: Noctuidae). V. Rearing procedures and life cycles of 36 species. Can Entomol 108:1345–1357
Homberg U, Montague RA, Hildebrand JG (1988) Anatomy of antenno-cerebral pathways in the brain of the sphinx moth Manduca sexta. Cell Tissue Res 254:255–281
Kanzaki R, Shibuya T (1986) Identification of the deutocerebral neurons responding to the sexual pheromone in male silkworm moth brain. Zool Sci 3:409–418
Kanzaki R, Arbas EA, Strausfeld NJ, Hildebrand JG (1989) Physiology and morphology of projection neurons in the antennal lobe of the male moth Manduca sexta. J Comp Physiol A 165:427–453
Koontz MA, Schneider D (1987) Sexual dimorphism in neuronal projections from the antennae of silk moths (Bombyx mori, Antheraea polyphemus) and the gypsy moth (Lymantria dispar). Cell Tissue Res 249:39–50
Löfstedt C, Van Der Pers JNC, Löfqvist J, Lanne BS, Appelgren M, Bergström G, Thelin B (1982) Sex pheromone components of the turnip moth, Agrotis segetum: Chemical identification, electrophysiological evaluation, and behavioural activity. J Chem Ecol 8:1305–1322
Löfstedt C, Linn jr CE, Löfqvist J (1985) Behavioural responses of male turnip months, Agrotis segetum, to sex pheromone in a flight tunnel and in the field. J Chem. Ecol 11:1209–1221
Malun D (1991a) Inventory and distribution of synapses of identified uniglomerular projection neurons in the antennal lobe of Periplaneta americana. J Comp Neurol 305:348–360
Malun D (1991b) Synaptic relationships between GABA-immunoreactive neurons and an identified uniglomerular projection neuron in the antennal lobe of Periplaneta americana: a double-labeling electron microscopic study. Histochemistry 96:197–207
Matsumoto SG, Hildebrand JG (1981) Olfactory mechanisms in the moth Manduca sexta: Response characteristics and morphology of central neurons in the antennal lobes. Proc R Soc London B 213:249–277
Olberg RM (1983) Interneurons sensitive to female pheromone in the deutocerebrum of the male silkworm moth, Bombyx mori. Physiol Entomol 8:419–428
Rospars JP (1983) Invariance and sex-specific variations of the glomerular organization in the antennal lobes of a moth, Mamestra brassicae, and a butterfly, Pieris brassicae. J Comp Neurol 220:80–96
Rospars JP, Hildebrand JG (1992) Anatomical identification of glomeruli in the antennal lobes of the male sphinx moth Manduca sexta. Cell Tissue 95:223–248
Schneider D (1992) 100 years of pheromone research. Naturwissenschaften 79:241–250
Tolbert LP, Hildebrand JG (1981) Organization and synaptic ultrastructural of glomeruli in the antennal lobes of the moth Manduca sexta: a study using thin sections and freeze-structure. Proc R Soc s London 213:279–301
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Hansson, B.S., Anton, S. & Christensen, T.A. Structure and function of antennal lobe neurons in the male turnip moth, Agrotis segetum (Lepidoptera: Noctuidae). J Comp Physiol A 175, 547–562 (1994). https://doi.org/10.1007/BF00199476
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DOI: https://doi.org/10.1007/BF00199476