Abstract
Arabidopsis thaliana (L.) Heynh. Columbia wild type and a root hair-less mutant RM57 were grown on iron-containing and iron-deficient nutrient solutions. In both genotypes, ferric chelate reductase (FCR) of intact roots was induced upon iron deficiency and followed a Michaelis-Menten kinetic with a K m of 45 and 54 μM FeIII-EDTA and a V max of 42 and 33 nmol Fe2+·(g FW)−1·min−1 for the wild type and the mutant, respectively. The pH optimum for the reaction was around pH 5.5. The approximately four fold stimulation of FCR activity was independent of formation of root hairs and/or transfer cells induced by iron deficiency. Iron-deficiency-induced chlorosis and the development of a rigid root habit disappeared when ferric chelate was applied to the leaves, while FCR activity remained unchanged. The time course of the responses to iron deficiency showed that morphological and physiological responses were controlled separately.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- FCR:
-
ferric chelate reductase
- FW:
-
fresh weight
References
Abadia, J. (1992) Leaf responses to Fe deficiency: A review. J. Plant Nutr. 15, 1699–1714
Ao, T.Y., Fan, F., Korcak, R.F., Faust, M. (1985) Iron reduction by apple roots. J. Plant Nutr. 8, 629–644
Bell, P.F., Chaney, R.L., Angle, J.S. (1988) Staining localization of ferric reduction on roots. J. Plant Nutr. 11, 1237–1252
Bienfait, H.F. (1987) Biochemical basis of iron efficiency reactions in plants. In: Iron transport in microbes, plants and animals, pp. 339–349, van der Helm, D., Neilands, J.B., Winkelmann, G., eds. Verlag Chemie, Weinheim
Bienfait, H.F. (1988) The Turbo reductase in plant plasma membranes. In: Plasma membrane oxidoreductases in control of animal and plant growth, pp. 89–98, Crane, F.L., Morré, D.J., Löw, H.E., eds. Plenum Press, New York
Brüggemann, W, Moog, P.R., Nakagawa, H., Janiesch, P., Kuiper, P.J.C. (1990) Plasma membrane-bound Fe3+ -EDTA reductase and iron deficiency in tomato Lycopersicon esculentum. Is there a Turbo reductase? Physiol. Plant. 79, 339–346
Buckhout, T.J., Luster, D.G., Chaney, R.L. (1989) Iron-stress induced redox activity in tomato (Lycopersicon esculentum Mill.) is localized on the plasma membrane. Plant Physiol. 90, 151–156
Chaney, R.L. (1989) Kinetics of ferric chelate reduction by roots of iron-deficient peanut (Arachis hypogea). Acta Bot. Neerl. 38, 153–163
Chaney, R.L., Brown, J.D., Tiffin, L.O. (1972) Obligatory reduction of ferric chelates in iron uptake by soybeans. Plant Physiol. 50, 208–213
Chaney, R.L., Chen, Y., Green, C.E., Holden, M.J., Bell, P.F., Luster, D.G., Angle, J.S. (1992) Root hairs on chlorotic tomatoes are an effect of chlorosis rather than part of the adaptive Fe-stress response. J. Plant Nutr. 15, 1857–1875
Doddema, H. (1978) Uptake of nitrate by chlorate resistant mutants of Arabidopsis thaliana (L.) Heynh. Ph. D. thesis. University of Groningen, Groningen, Netherlands
Dolcet-Sanjuan, R., Mok, D.W.S., Mok, M.C. (1992) Characterization and in vitro selection for iron efficiency in Pyrus and Cydonia. In Vitro Cell Dev. Biol. 28P, 25–29
Kramer, D., Römheld, V, Landsberg, E., Marschner, H. (1980) Induction of transfer cell formation by iron deficiency in the root epidermis of Helianthus annuus L. Planta 147, 335–339
Landsberg, E.C. (1982) Transfer cell formation by iron deficiency in the root epidermis: a prereqisite for Fe efficiency? J. Plant Nutr. 5, 415–432
Landsberg, E.C. (1986) Function of rhizodermal transfer cells in Fe stress response mechanism of Capsicum annum L. Plant Physiol. 82, 511–517
Landsberg, E.C. (1989) Proton efflux and transfer cell formation as a responses to Fe deficiency of soybean in nutrient solution culture. Plant Soil 114, 53–61
Marschner, H., Römheld, V., Horst, W.J., Martin, P. (1986) Root-induced changes in the rhizosphere: Importance for the mineral nutrition of plants. Z. Pflanzenernähr. Bodenk. 149, 441–456
Misra, R.K., Alston, A.M., Dexter, A.R. (1988) Role of root hairs in phosphorus depletion from a macrostructured soil. Plant Soil 107, 11–18
Moog, P.R., Brüggemann, W. (1994) Iron reductase systems at the plant plasma membrane. Plant Soil 165, in press
Moog, P.R., Janiesch, P. (1990) Root growth and morphology of Carex species as influenced by oxygen deficiency. Funct. Ecol. 4, 201–208
Reynolds, E. (1963) The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J. Cell Biol. 17, 208–212
Römheld, V. (1987) Existence of two different strategies for the acquisition of iron in higher plants. In: Iron transport in microbes, plants and animals, pp 353–374, van der Helm, D., Neilands, J.B., Winkelmann, G., eds. Verlag Chemie, Weinheim
Römheld, V., Kramer, D. (1983) Relationship between proton efflux and rhizodermal transfer cells induced by iron deficiency. Z. Pflanzenphysiol. 113, 73–83
Römheld, V., Marschner, H. (1981) Iron deficiency stress induced morphological and physiological changes in root tips of sunflower. Physiol. Plant. 53, 354–360
Römheld, V., Marschner, H. (1983) Mechanism of iron uptake by peanut plants I. FeIII reduction, chelate splitting and release of phenolics. Plant Physiol. 71, 949–954
Rosenfield, C.L., Reed, D.W., Kent, M.W. (1991) Dependency of iron reduction on development of a unique root morphology in Ficus benjamina L. Plant Physiol. 95, 1120–1124
Schiefelbein, J.W., Somerville, C. (1990) Genetic control of root hair development in Arabidopsis thaliana. Plant Cell 2, 235–243
Schmidt, W., Janiesch, P. (1991) Ferric reduction by Geum urbanum: A kinetic study. J. Plant Nutr. 14, 1023–1034
Schmidt, W., Janiesch, P., Brüggemann, W. (1990) FeEDTA reduction in roots of Plantago lanceolata by a NADH-dependent plasma membrane-bound redox system. J. Plant Physiol. 136, 51–55
Sijmons, P.C., Bienfait, H.F. (1983) Source of electrons for extracellular Fe(III) reduction in iron-deficient bean roots. Physiol. Plant. 59, 409–415
Author information
Authors and Affiliations
Additional information
Thanks are due to Klaas Sjollema (Department of Electronmicroscopy, University of Groningen, The Netherlands) for help with the electron microscopy sample preparation and especially to Dr. Uwe Santore (Heinrich-Heine-University for electron microscopy. This work was supported by the SCIENCE programm of the European community; P.R.M.) and a Personal Research Grant by the Ministerium für Wissenschaft und Forschung of Nordrhein-Westfalen (P.R.M.) and last, not least by the productive discussions in ECOTRANS B.V.
Rights and permissions
About this article
Cite this article
Moog, P.R., van der Kooij, T.A.W., Brüggemann, W. et al. Responses to iron deficiency in Arabidopsis thaliana: The Turbo iron reductase does not depend on the formation of root hairs and transfer cells. Planta 195, 505–513 (1995). https://doi.org/10.1007/BF00195707
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00195707