Abstract
The β1-, β2-, and β3-tubulin genes have been mapped by in situ hybridization on the polytene chromosomes of 11 selected species (15 strains) belonging to the Drosophila montium subgroup. Although the hybridization pattern among the strains of the same species does not differ, this pattern is significantly different among the species. The β-tubulin genes in the montium subgroup seem to be organized in a cluster, or in a semi-cluster, or are completely dispersed. The clustered arrangement is found in the North-Oriental sibling species D. auraria, D. triauraria, and D. quadraria. The semi-clustered arrangement, wherein the β1 and β2 genes are located at the same locus while β3 is at a different one, appears in the South-Oriental species D. bicomuta, D. serrata, and D. birchii, as well as in the Afrotropical species D. diplacantha and D. seguyi. The complete separation of the genes is observed in the Indian species D. kikkawai and D. jambulina and in the Afrotropical species D. vulcana. Based on the above results, a possible mode of evolution of the β-tubulin genes in the montium subgroup is attempted. In addition, phylogenetic relationships among the montium species are discussed.
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References
Ashbumer M (1989) Drosophila:a laboratory handbook. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Ayala JF (1965) Sibling species of the Drosophila serrata group. Evolution 19:538–545
Baimai V (1970) Chromosomal polymorphism in Drosophila birchii. J Hered 61:23–34
Biolojan S, Falkenburg D, Renkawitz-Pohl R (1984) Characterization and developmental expression of β-tubulin genes in Drosophila melanogaster. EMBO J 3:2543–2548
Bock IR (1980) Current status of the Drosophila melanogaster species group (Diptera). System Entomol 5:341–346
Bock IR, Wheeler MR (1972) The Drosophila melanogaster species group. Univ Tex Publ 7213:1–102
Cleveland DW, Sullivan KF (1985) Molecular biology and genetics of tubulin. Annu Rev Biochem 54:331–365
Fackenthal JD, Turner FR, Raff EC (1993) Tissue-specific microtubule functions in Drosophila spermatogenesis require the β2-tubulin isotype-specific carboxy terminus. Dev Biol 158:213–227
Gash A, Hinz U, Leiss D, Renkawitz-Pohl R (1988) The expression of β1 and β3 tubulin genes of Drosophila melanogaster is spatially regulated during embryogenesis. Mol Gen Genet 211:8–16
Gash A, Hinz U, Renkawitz-Pohl R (1989) Intron and upstream sequences regulate expression of the Drosophila β-tubulin gene in the visceral and somatic musculature, respectively. Proc Natl Acad Sci USA 86:3215–3218
Kalantzi-Makri MC, Margaritis LH, Sourdis J (1985) Phylogenetic relationships within the montium species subgroup of the genus-Drosophila (Sophophora) based on electrophoretic data of the major chorion proteins. Ann Soc Entomol Fr (New Set) 21:357–366
Kastritsis CD, Scouras ZG, Ashbumer M (1986) Duplications in the polytene chromosomes of Drosophila auraria. Chromosoma 93:381–385
Kim BK (1988) Phylogenetic relationships of the seven species of the Drosophila auraria complex by two dimensional electrophoresis. Korean J Genet 10:77–84
Kim BK, Watanabe TK, Kitagawa O (1989) Evolutionary genetics of the Drosophila montium subgroup. I. Reproductive isolations and the phylogeny. Jpn J Genet 64:177–190
Kimura MT (1987) Habitat differentiation and speciation in the Drosophila auraria species complex (Diptera, Drosophilidae). Kontuy 55:429–426
Lamnissou K, Zouros E (1989) Interspecific ovarian transplantation in Drosophila: vitellogenin uptake as an index of evolutionary relatedness. Heredity 63:29–35
Leiss D, Hinz U, Gash A, Mertz R, Renkawitz-Pohl R (1988) β-tubulin expression characterizes the differentiating mesodermal germ layer during Drosophila embryogenesis. Development 104:525–531
Lemeunier F, David JR, Tsacas L, Ashbumer M (1986) The melanogaster species group. In: Ashbumer M, Carson HL, Thompson J (eds) The genetics and biology of Drosophila. Academic Press, London, vol 3, pp 147–256
Loukas M, Delidakis C, Kafatos FC (1986) Genomic blot hybridization as a tool for phylogenetic analysis: evolutionary divergence in the genus Drosophila. J Mol Evol 24:174–188
Luduena RF (1993) Are tubulin isotypes functionally significant. Mol Biol Cell 4:445–457
Mavragani-Tsipidou P, Scouras ZG (1991) Developmental changes in fat body and midgut chromosomes of Drosophila auraria. Chromosome 100:443–452
Mavragani-Tsipidou P, Kyrpides N, Scouras ZG (1990) Evolutionary implications of duplications and Balbiani rings in Drosophila: a study of Drosophila serrata. Genome 33:478–485
Mavragani-Tsipidou P, Scouras ZG, Natsiou-Voziki A (1992a) The Balbiani ring and the polytene chromosomes of Drosophila bicornuta. Genome 35:64–67
Mavragani-Tsipidou P, Scouras ZG, Haralampidis K, Lavrendiadou S, Kastritsis CD (1992b) The polytene chromosomes of Drosophila triauraria and D. quadraria, sibling species of D. auraria. Genome 35:318–326
Mavragani-Tsipidou P, Zambetaki A, Kleanthous K, Pangou E, Scouras ZG (1994) Cytotaxonomic differentiation of the Afrotropical Drosophila montium subgroup: D. diplacantha and D. seguyi—the major role of reverse tandem duplications. Genome 37 (in press)
Michiels F, Falkenburg D, Muller AM, Hinz U, Otto U, Bellmann R, Glatzer KH, Brand R, Biolojan S, Renkawitz-Pohl R (1987) Testes specific β2 tubulins are identical between Drosophila melanogaster and D. hydei but differ from the ubiquitous β1 tubulin. Chromosome 95:387–395
Michiels F, Gasch A, Kaltschmidt B, Renkawitz-Pohl R (1989) A 14 by promoter element directs the testis specificity of the Drosophila β2-tubulin gene. EMBO J 8:1559–1564
Natzle JE, McCarthy BJ (1984) Regulation of Drosophila α and β tubulin genes during development. Dev Biol 104:187–198
Ohno S (1970) Evolution by gene duplication. Springer-Verlag, NY
Pardue ML (1986) Drosophila a practical approach. In Roberts DB (ed) In situ hybridization to DNA of chromosomes and nuclei. IRL Press, Oxford, p 111
Parkash R, Rajput PS (1983) Photomap of the salivary gland chromosomes of D. jambulina (Parshad and Paika). DIS 59:96–98
Pissios P, Scouras ZG (1993) Mitochondrial DNA evolution in the montium species subgroup of Drosophila. Mol Biol Evol 10:375–382
Roy S, Lakhotia SC (1977) Photomap of salivary chromosomes of Drosophila kikkawai. DIS 52:118–119
Sanchez F, Natzle JE, Cleveland DW, Kirscher MW, McCarthy BJ (1980) A dispersed multigene family encoding tubulin in Drosophila melanogaster. Cell 22:845–854
Scouras ZG, Kastritsis CD (1984) Balbiani rings and puffs of the polytene chromosomes of Drosophila auraria. Chromosoma 89:96–106
Scouras ZG, Mavragani-Tsipidou P (1992) Balbiani rings in Drosophila: the Balbiani ring 1 is a common characteristic in several montium species. Cytobios 69:97–100
Scouras ZG, Milioni D, Yiangou M, Duchene M, Domdey H (1994) The β-tubulin genes of Drosophila auraria are arranged in a cluster. Curr Genet 25:84–87
Tsacas S, Tsacas L (1984) A phenetic tree of eighteen species of the melanogaster group of Drosophila using allozyme data as compared with the classification based on other criteria. Genetica (Hague) 64:139–144
Triantaphyllidis CD, Kastritsis CD (1976) Acid phosphatases in Drosophila auraria. Experientia 32:1277–1278
Triantaphyllidis CD, Panourgias JN, Scouras ZG (1978) Enzyme variation and phylogenetic relationships among six species of montium subgroup of the Drosophila melanogaster species group. Genetica (Hague) 48:227–233
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Correspondence to: Z.G. Scouras
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Drosopoulou, E., Scouras, Z.G. The β-tubulin gene family evolution in the Drosophila montium subgroup of the melanogaster species group. J Mol Evol 41, 293–298 (1995). https://doi.org/10.1007/BF00186541
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DOI: https://doi.org/10.1007/BF00186541