Abstract
In the bivalve mollusc Pecten maximus, the size of the mitochondrial DNA molecules ranges from 20 to 25.8 kbp. This variability is mainly correlated with the occurrence of a variable domain composed with two to five 1.6-kbp repeated units tandemly arrayed in the genome. DNA fragments spanning the 1,586-base-pair-long repeated element and the nearest flanking gene have been cloned and sequenced. This sequence was analyzed regarding its base composition and potential secondary structures. The repeated unit domain was positioned and oriented with regard to the known flanking gene. It ends 2 base pairs upstream relative to the beginning of the tRNAgly gene. The peculiar properties of the repeated unit were compared with those of the 1,442-bp repeated element found in the mitochondrial genome of the deep sea scallop Placopecten magellanicus. This comparison provided evidence for the absence of nucleotide conservation, except for a small sequence engaged in a secondary structure, but argued for a strong pressure maintaining domains with specific nucleotide content. A possible role for the conserved sequence is discussed.
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Boyce TM, Zwick ME, Aquadro CF (1989) Mitochondrial DNA in the bark weevils: size, structure and heteroplasmy. Genetics 123:825–836
Chang DD, Clayton DA (1986) Identification of primary transcriptional strat sites of mouse mitochondrial DNA: accurate in vitro initiation of both heavy- and light-strand transcripts. Mol Cell Biol 6:1446–1453
Clary DO, Wolstenholme DR (1985) The mitochondrial DNA molecule of Drosophila yakuba: nucleotide sequence, gene organization, and genetic code. J Mol Evol 22:252–271
Clayton DA (1982) Replication of animal mitochondrial DNA. Cell 28:693–705
Clayton DA (1984) Transcription of the mammalian mitochondrial genome. Annu Rev Biochem 53:573–594
Cornuet JM, Garnery L, Solignac M (1991) Putative origin and function of the intergenic region between COI and COII of Apis mellifera L. mitochondrial DNA. Genetics 1128:393–403
Dessert P, Fondrat C, Valencien C, Mugnier C (1990) Bisance: French service for access to biomolecular sequence data-bases. Comput Appl Biosci 6:355–356
Fuller KM, Zouros E (1993) Dispersed discrete length polymorphism of mitochondrial DNA in the scallop Placopecten magellanicus (Gmelin). Curr Genet 23:365–369
Gjetvaj B, Cook DI, Zouros E (1992) Repeated sequences and large-scale size variation of mitochondrial DNA: a common feature among scallops (Bivalvia: Pectinidae). Mol Biol Evol 9:106–124
Goddard JM, Wolstenholme DR (1980) Origin and direction of replication in mitochondrial DNA molecules from the genus Drosophila. Nucleic Acids Res 8:741–757
Hoffmann RJ, Boore JL, Brown WM (1992) A novel mitochondrial genome organization for the bleu mussel Mytulis edulis. Genetics 131:397–412
Hyman BC, Beck JL, Weiss KC (1988) Sequence amplification and gene rearrangement in parasitic nematode mitochondrial DNA. Genetic 120:707–712
La Roche J, Snyder M, Cook DI, Fuller K, Zouros E (1990) Molecular characterization of a repeat element causing large-scale size variation in the mitochondrial DNA of the sea scallop Placopecten magellanicus. Mol Biol Evol 7:45–64
Miklos GLG, Yamamoto MT, Davies J, Pirrotta V (1988) Microcloning revels a high frequency of repetitive sequences characteristic of chromosome 4 and the b heterochromatin of Drosophila melanogaster. Proc Natl Acad Sci USA 85:2015–2055
Monforte A, Barrio E, Latorre A (1993) Characterization of the length polymorphism in the A+T-rich region of the Drosophila obscura group species. J Mol Evol 36:214–223
Moritz C, Brown WM (1986) Tandem duplications of D-loop and ribosomal RNA sequences in lizard mitochondrial DNA. Science 233:1425–1427
Rand DM (1993) Endotherms, ectotherms, and mitochondrial genomesize variation. J Mol Evol 37:281–295
Rand DM, Harrison RG (1989) Molecular population genetics of mtDNA size variation in crickets. Genetics 212:551–569
Rigaa A, Monnerot M, Le Gal Y, Sellos D (1993) Mapping and repeated sequence organization of mitochondrial DNA in scallop, Pecten maximus. Mol Marine Biol Biot 2:218–224
Solignac M, Monnerot M, Mounolou J-C (1986) Concerted evolution of sequence repeats in Drosophila mitochondrial DNA. J Mol Evol 24:53–60
Vaury C, Bucheton A, Pelison A (1989) The b heterochromatic sequences flanking the I elements are themselves defective transposable elements. Chromosoma 98:215–224
Walberg MW, Clayton DA (1981) Sequence and properties of the human KB cell and mouse L cell D-loop regions of mitochondrial DNA. Nucleic Acids Res 9:5411–5421
Wallis GP (1987) Mitochondrial DNA insertion polymorphism and germ line heteroplasmy in the Trutus cristatus species complex. Heredity 58:229–238
Zamarocsy M, Faugeron-Fonty G, Baldacci G, Goursot R, Bernardi G (1984) The on sequences of the mitochondrial genome of a wildtype yeast strain: number, location, orientation and structure. Gene 1152:157–439
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Rigaa, A., Monnerot, M. & Sellos, D. Molecular cloning and complete nucleotide sequence of the repeated unit and flanking gene of the scallop Pecten maximus mitochondrial DNA: Putative replication origin features. J Mol Evol 41, 189–195 (1995). https://doi.org/10.1007/BF00170672
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DOI: https://doi.org/10.1007/BF00170672