Abstract
Study of polymorphisms in the eukaryotic genome is an important way to discover the evolutionary relationships between species. Artemia (Crustacea, Anostraca) offers a very interesting model for evolutionary studies. In fact the genus, distributed all over the world in hundreds of known biotopes, comprises both bisexual sibling species and parthenogenetic populations easily available from the Artemia Reference Center of Ghent. In spite of great interest in it and its extensive use in aquaculture, little is known about relationships between the different species and intraspecific populations. Recently it has been demonstrated that polymorphisms in genomic fingerprints generated by arbitrarily primed polymerase chain reaction (PCR) can distinguish between strains in many organisms. We have used this technique to estimate the phylogenetic relationships existing between 14 populations living in the American continent, in the Mediterranean area, and in China. The principal coordinate analysis (PCO) obtained from 86 random amplified polymorphic DNA (RAPD) markers indicates that the populations analyzed can be divided into homogeneous clusters representing the four known bisexual species—the American A. franciscana and A. persimilis, the Mediterranean A. salina, and the A. species from China.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Abreu-Grobois FA (1987) A review of the genetics of Artemia. In: Sorgeloos P, Bengston DA, Decleir W, Jaspers E (eds) Artemia research and its applications, vol 1. Universa Press, Wetteren, Belgium, pp 61–99
Badaracco G, Baratelli L, Ginelli E, Meneveri R, Plevani P, Valsasnini P, Barigozzi C (1987) Variation in repetitive DNA and heterochromatin in the genus Artemia. Chromosoma 95:71–75
Badaracco U, Tubiello G, Benfante R, Cotelli F, Maiorano D, Landsberger N (1991) Highly repetitive DNA sequence in parthenogenetic Artemia. J Mol Evol 32:31–36
Barigozzi C (1980) Genus Artemia: problems of systematics. In: Persoone G, Sorgeloos P, Roels O, Jaspers E (eds) The brine shrimp Artemia. vol I. Edited by Universa Press, Wetteren, Belgium, pp 147–153
Barigozzi C (1989) Cytogenetics and speciation of the brine shrimp Artemia. Atti Ace Lincei, vol XIX
Barigozzi C, Badaracco G, Plevani P, Baratelli L, Profeta S, Ginelli E, Meneveri R (1984) Heterochromatin in the genus Artemia. Chromosoma 90:332–337
Beardmore J, Abreu-Grobois FA (1983) Taxonomy and evolution in the brine shrimp Anemia. In: Oxford GS, Ro D (eds) Protein polymorphism: adaptive and taxonomic significance. Academic Press, London, pp 153–164
Bowen ST, Buoncristiani MR, Carl JR (1988) Artemia habitats: ion concentrations tolerated by one superspecies. Hydrobiologia 158: 201–214
Brauer A (1893) cited by Barigozzi C (1980) Zur Kenntnis der Reifung des parthenogenetisch rich entwickelnden Eies von Artemia salina. Arch Mikrosk Anat Entwicklungsmech Bonn 43:162–222
Browne RA, Bower ST (1991) Taxonomy and population genetics of Artemia. In: Browne RA, Sorgeloos P, Trotman CNA (eds) Artemia biology. CRC Press, Boca Raton, FL, pp 221–235
Browne RA (1992) Population genetics and ecology of Artemia: insights into parthenogenetic reproduction. TREE 7:232–237
Cruces J, Wonenburger MLG, Diaz-Guerra M, Sebastiàn J, Renart J (1986) Satellite DNA in the crustacean Artemia. Gene 44:341–345
Demeke T, Adams RP, Chibbar R (1992) Potential taxonomic use of random amplified polymorphic DNA (RAPD): a case study in Brassica. Theor Appl Genet 84:990–994
Gower JC (1971) A general coefficient of similarity and some of its properties. Biometrics 27:857–874
Koller B, Lehamann A, McDermott JM, Gessler C (1993) Identification of apple cultivars using RAPD markers. Theor Appl Genet 84:901–904
Landsberger N, Cancelli S, Carettoni D, Barigozzi C, Badaracco G (1992) Nucleotide variation and molecular structure of the heterochromatic repetitive AluI DNA in the brine shrimp Artemia franciscana. J Mol Evol 35:486–491
Perez ML, Valverde JR, Batuecas B, Amat F, Marco R, Garrese R (1994) Speciation in the Artemia genus: Mitochondrial DNA analysis of bisexual and parthenogenetic brine shrimps. J Mol Evol 38:156–168
Pilla EJS, Beardmore JA (1994) Genetic and morphometric differentiation in Old World bisexual species of Artemia (the brine shrimp). Heredity 73:47–56
Stiles JF, Lemme C, Sondur S, Morshidi MB, Manshardt R (1993) Using randomly amplified polymorphic DNA for evaluating genetic relationships among papya cultivars. Theor Appl Genet 85: 697–701
Vanhaecke P, Tackaert W, Sorgeloos P (1987) The biogeography of Artemia: an updated review. In: Sorgeloos P, Bengston DA, Decleir W, Jaspers E (eds) Artemia research and its applications, vol 1. Universa Press, Wetteren, Belgium, pp 128–155
Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18:7213–7218
Welsh J, Petersen C, McClelland M (1991) Polymorphisms generated by arbitrarily primed PCR in the mouse: application to strain identification and genetic mapping. Nucleic Acids Res 19:303–306
Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Scott VT (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Badaracco, G., Bellorini, M. & Landsberger, N. Phylogenetic study of bisexual Artemia using random amplified polymorphic DNA. J Mol Evol 41, 150–154 (1995). https://doi.org/10.1007/BF00170666
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00170666