Abstract
A laboratory-scale system for controlled dynamic solid substrate fermentation was developed and tested. The fermentation takes place in a stainless steel discontinuously rotating drum reactor, under controlled conditions of temperature, gas composition, relative humidity and direction and rate of rotation. The system was tested on a model fermentation of soya beans with Rhizopus oligosporus. In contrast to the traditional tempe fermentation, a granular product is obtained and build-up of heat and mass gradients is restricted. Despite the discontinuous rotation, the fungal growth continues, as evidenced by the production of heat. The rate of cooling depends on the temperature of the gas flushed through the reactor, the gas flow rate and the lenght of the rotation period. As a consequence of the homogeneous temperature control, the fungal heat development continued up to 70 h of fermentation. This is in clear contrast with the traditional tempe fermentation, which is already limited after 36 h by its own heat accumulation.
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Aidoo KE, Hendry R, Wood BJB (1982) Solid substrate fermentations. Ad Appl Microbiol 28:201–237
Alvarez-Martinez LR (1987) Modelling fungal Rhizopus oligosporus growth on extruded corn by solid substrate fermentation. Ph. D. Thesis, Colorado State University, Dissertation Abstracts International B49, 1284
Barstow LM, Dale BE, Tengerdy RP (1988) Evaporative temperature and moisture control in solid substrate fermentation. Biotechnol Tech 2:237–242
Cannel E, Moo-Young M (1980) Solid-state fermentation systems. Process Biochem 15:2–7, 24–28
Kargi F, Curme JA (1985) Solid state fermentations of sweet sorghum to ethanol in a rotary-drum fermentor. Biotechnol Bioeng 17:1122–1125
Larroche C, Gros J-B (1986) Spore production of Penicillium roquefortii in fermentors filled with buckwheat seeds: batch and semi-continuous cultivation. Appl Microbiol Biotechnol 24:134–139
Lindenfelser LA, Ciegler A (1975) Solid substrate fermentor for ochratoxin A production. Appl Microbiol 29:323–327
Mitchell DA, Doelle HW, Greenfield PH (1988) Improvement of growth of Rhizopus oligosporus on a model solid substrate. Biotechnol Lett 10:497–502
Mudgett RE (1986) Solid-state fermentations. In: Demain AL, Solomon NA (eds) Manual of industrial microbiology and biotechnology. American Society for Microbiology, Washington, D. C. pp 66–83
Nout MJR, Rombouts FM (1990) Recent developments in tempe research. J Appl Bacteriol 69:609–633
Nout MJR, Dreu MA de, Zuurbier AM, Bonants-van Laarhoven TMG (1987) Ecology of controlled soya bean acidification for tempe manufacture. Food Microbiol 4:165–172
Rathbun BL, Shuler ML (1983) Heat and mass transfer effects in static solid substrate fermentation: design of fermentation chambers. Biotechnol Bioeng 25:929–938
Ryoo D, Murphy VG, Karim MN, Tengerdy RP (1991) Evaporative temperature and moisture control in a rocking reactor for solid substrate fermentation. Biotechnol Tech 5:19–24
Shibasaki N, Hirose K, Yonemoto T, Tadaki T (1992) Suspension culture of Nicotiana tabacum cells in a rotary drum bioreactor. J Chem Technol Biotechnol 53:359–363
Shurtleff W, Aoyagi A (1980) The book of tempeh, volume II: tempe production. New-Age Foods, Lafayette, California
Silman RW (1980) Enzyme formation during solid-substrate fermentation in rotating vessels. Biotechnol Bioeng 22:411–420
Smith JE, Aidoo KE (1988) Growth of fungi on solid substrates. In: Berry DR (ed) Physilogy of industrial fungi. Blackwell Scientific Publications, Oxford, pp 249–269
Weiland P, Scholz CH (1990) Ethanol fermentation in a solid-phase process. Dechema Biotechnology Conferences 4. VCH Verlagsgesellschaft, Weinheim, pp 855–858
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Correspondence to: M. J. R. Nout
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de Reu, J.C., Zwietering, M.H., Rombouts, F.M. et al. Temperature control in solid substrate fermentation through discontinuous rotation. Appl Microbiol Biotechnol 40, 261–265 (1993). https://doi.org/10.1007/BF00170377
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DOI: https://doi.org/10.1007/BF00170377