Abstract
Anaerobic fermentation of glucose (20 g/l) by Saccharomyces cerevisiae CBS 8066 was studied in a chemostat (dilution rate = 0.05−0.25 h−1) at different concentrations of the nitrogen source (5.00 g/l or 0.36 g/l ammonium sulphate). The ethanol yield (g ethanol produced/g glucose consumed) was found to be higher and the glycerol yield (g glycerol formed/g glucose consumed) lower during nitrogen limitation than under carbon limitation. The biomass yield on ATP (g dry weight biomass produced/mol ATP consumed) was consequently found to be lower during nitrogen-limited conditions.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Bauchop T, Elsden SR (1960) The growth of micro-organisms in relation to their energy supply. J Gen Microbiol 23:457–469
Blomberg A, Adler L (1992) Physiology of osmotolerance in fungi. Adv Microbiol Physiol 33:145–212
Bruinenberg PM, Dijken JP van, Scheffers WA (1983) A theoretical analysis of NADPH production and consumption in yeasts. J Gen Microbiol 129:953–964
Fiechter A, Fuhrmann F, Käppeli O (1981) Regulation of glucose metabolism in growing yeast cells. Adv Microbiol Physiol 22:123–183
Lagunas R, Gancedo JM (1973) Reduced pyridine-nucleotides balance in glucose-growing Saccharomyces cerevisiae. Eur J Biochem 37:90–94
Lagunas R, Ruiz E (1988) Balance and consumption of ATP in ammonium-starved Saccharomyces cerevisiae. J Gen Microbiol 134:2507–2511
Larsson C, Stockar U von, Marison I, Gustafsson L (1993) Growth and metabolism of Saccharomyces cerevisiae in chemostat cultures under carbon-, nitrogen-, or carbon- and nitrogen- limiting conditions. J Bacteriol 175:4809–4816
Lidén G, Jacobsson V, Niklasson C (1993) The effect of carbon dioxide on xylose fermentation by Pichia stipitis. Appl Biochem Biotechnol 38:27–40
Neijssel OM, Tempest DW (1987) Relationships between nutrient status of the environment and metabolic fluxes in microorganisms. Dechema Monogr 105:29–42
Neijssel OM, Tempest DW (1990) The role of futile cycles in the energetics of bacterial growth. Biochim Biophys Acta 1018:252–255
Nilsson A, Larsson C, Gustafsson L (1995) Catabolic capacity of Saccharomyces cerevisiae in relation to the physiological state and maintenance requirement. Thermochim Acta (in press)
Oelz R, Larsson K, Adler L, Gustafsson L (1993) Energy flux and osmoregulation of Saccharomyces cerevisiae grown in chemostats under NaCl stress. J Bacteriol 175:2205–2213
Oura E (1977) Reaction products of yeast fermentations. Proc Biochem 12:19–21, 35
Schatzmann H (1975) Anaerobes Wachstum von Saccharomyces cerevisiae. Regulatorische Aspekte des glycolytischen und respirativen Stoffwechsels. Diss. 5504, ETH, Zurich, Zwitzerland
Stouthamer AH (1979) The search for correlation between theoretical and experimental growth yields. Int Rev Biochem 21:1–47
Van Urk H, Mak PR, Scheffers WA, Dijken JP van (1988) Metabolic responses of Saccharomyces cerevisiae CBS 8066 and Candida utilis CBS 621 upon transition from glucose limitation to glucose excess. Yeast 4:283–291
Verduyn C, Postma E, Scheffers WA, van Dijken JP (1990) Physiology of Saccharomyces cerevisiae in anaerobic glucose-limited chemostat culture. J Gen Microbiol 136:395–403
Visser W, Scheffers WA, Batenburg-van der Vegte WH, Dijken JP van (1990) Oxygen requirements of yeasts. Appl Environ Microbiol 56:3785–3792
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lidén, G., Persson, A., Niklasson, C. et al. Energetics and product formation by Saccharomyces cerevisiae grown in anaerobic chemostats under nitrogen limitation. Appl Microbiol Biotechnol 43, 1034–1038 (1995). https://doi.org/10.1007/BF00166921
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00166921