Abstract
Adventitious shoot regeneration was observed using leaf-petiole explants from shoot-proliferating cultures of ‘Comet’ red raspberry (Rubus idaeus L.). A maximum regeneration rate of 70% (3.7 shoots/explant) was obtained using 4.5–9.1 μM (1–2 mg l−1) N-phenyl-N′-1,2,3-thiadiazol-5-ylurea (thidiazuron or TDZ) with 2.5–4.9 μM (0.5–1 mg l−1) 1H-indole-3-butanoic acid (IBA) or 2.3 μM (0.5 mg l−1) TDZ with 4.9 μM (1 mg l−1) IBA in modified Murashige-Skoog medium. TDZ was more effective than N-(phenylmethyl)-1H-purin-6-amine (BA) at promoting regeneration in combinations tested with IBA (maximum 50% regeneration rate; 1.8 shoots/explant). Variation in the agar concentration or incubation temperature, orientation or scoring of the leaf-petiole explants and use of separate leaf or petiole explants had no effect on shoot regeneration. Incubation in the dark for 1, 2 or 3 weeks prior to growth in the light did not influence the percent regeneration rate but depressed the number of adventitious shoots. Explant source, from micropropagated shoots or greenhouse-grown plants, had an effect on shoot regeneration that was genotype dependent. Only 8 of 22 (36%) raspberry cultivars were capable of regeneration from leaf explants derived from greenhouse-grown plants.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
DonnellyDJ, Stace-SmithR & MellorFC (1980) In vitro culture of three Rubus species. Acta Hort 112: 69–75
DonnellyDJ, SkeltonFE & DaubenyHA (1986) External leaf features of tissue cultured ‘Silvan’ blackberry. HortScience 21: 306–308
FeuchtW, SchmidPPS, SchimmelpfengH, Theiler-HedtrichC & SchmittA (1985) In vitro vermehrte himbeeren im großversuch: Abweicherquote vom sortentypus von 0.68 prozent und enzym-muster der atypischen früchte. Erwerbsobstbau 27: 167–169
FiolaJA & SwartzHJ (1986) Somatic embryogenesis, organogenesis and proliferation in vitro from Rubus embryos. Acta Hort. 183: 91–98
FiolaJA, HassanMA, SwartzHJ, BorsRH & McNicolsR (1990) Effect of thidiazuron, light fluence rates and kanamycin on in vitro shoot organogenesis from excised Rubus cotyledons and leaves. Plant Cell Tiss. Org. Cult. 20: 223–228
GrahamJ, McNicolRJ & KumarA (1990) Use of the GUS gene as a selectable marker for Agrobacterium-mediated transformation of Rubus. Plant Cell Tiss. Org. Cult. 20: 35–39
JamesDJ, KnightVH & ThurbonIJ (1980) Micropropagation of red raspberry and the influence of phloroglucinol. Scientia Hort. 12: 313–319
LiuZR & SanfordJC (1988) Plant regeneration by organogenesis from strawberry leaf and runner tissue. HortScience 23: 1057–1059
McNicolRJ & GrahamJ (1990) In vitro regeneration of Rubus from leaf and stem segments. Plant Cell Tiss. Org. Cult. 21: 45–50
MikamiT, SudohR, NagaoE & KimoshitaT (1989) Genotypic variation in the in vitro morphogenesis from leaf explants of Beta vulgaris L. and B. maritima L. Euphytica 40: 271–273
MurashigeT & SkoogF (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
SwartzHJ, BorsR, MohamedF & NaessSK (1990) The effect of in vitro pretreatments on subsequent shoot organogenesis from excised Rubus and Malus leaves. Plant Cell Tiss. Org. Cult. 21: 179–184
WehnerTC & LocyRD (1981) In vitro adventitious shoot and root formation of cultivars and lines of Cucumis sativus L. HortScience 16: 759–760
ZivM, MeirG & HalevyAH (1983) Factors influencing the production of hardened glaucous carnation plantlets in vitro. Plant Cell Tiss. Org. Cult. 2: 55–65
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cousineau, J.C., Donnelly, D.J. Adventitious shoot regeneration from leaf explants of tissue cultured and greenhouse-grown raspberry. Plant Cell Tiss Organ Cult 27, 249–255 (1991). https://doi.org/10.1007/BF00157588
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00157588