Summary
31P nuclear magnetic resonance has been employed to monitor noninvasively Saccharomyces cerevisiae anaerobic glucose metabolism in suspended and immobilized cells. Results show that cell entrapment in Ca-alginate beads alters cell metabolism compared to that in suspended cells. Assuming similar intracellular ionic strength, differences in intracellular phosphate chemical shift indicate that the internal pH of the immobilized cells is lower than the suspended cell internal pH. This result is consistent with higher ethanol production rates exhibited by immobilized yeast.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bailey J. E. and Ollis D. F. (1986) In: Biochemical Engineering Fundamentals 2nd. Ed.. McGraw-Hill, pp. 212–220.
Banuelos M., Gancedo C., and Gancedo J. (1977). J. Biol. Chem. 252, 6394–6398.
Chotani G. and Constantinides A. (1984). Biotechn. Bioeng. 26, 217–220.
den Hollander J. A., Ugurbil K., Brown T. R., and Shulman R. G. (1981). Biochemistry 20, 5871–5880.
den Hollander, J. A., Ugurbil K., Brown T. R., Bednar M., Redfield C., and Shulman R. G. (1986). Biochemistry 25, 203–211.
Doran P. and Bailey J. E. (1986). Biotechn. Bioeng. 28, 73–87.
Foxall D. L. and Cohen J. S. (1983). J. Mag. Res. 52, 346–349.
Karczmar G. S., Koretsky A. P., Bissell M. J., Klein M. P., and Weiner M. W. (1983). J. Mag. Res. 53, 123–128.
Kosow D. and Rose I. (1971). J. Biol. Chem. 246, 2618–2625.
Laurent M. and Seydoux F. (1977). Bioch. Biophys. Res. Comm. 78, 1289–1295.
McGhee J. E., St.Julian G., and Detroy R. W. (1982). Appl. Envirom. Microbiol. 44, 19–24.
Navon G., Shulman R., Yamane T., Eccleshall T., Lam K., Baronotsky J., and Marmur J. (1979). Biochemistry 18, 4487–4499.
Nicolay K., Scheffers W. A., Bruinenberg P. M., and Kaptein R. (1982). Arch. Microbiol. 133, 83–89.
Salhany J., Yamane T., Shulman R., and Ogawa S. (1975). Proc. Natl. Acad. Sci. USA 72, 4966–4970.
Shanks J. V. and Bailey J. E. (1986). in preparation.
Sitton D. and Gaddy J. (1980). Biotechn. Bioeng. 22, 1735–1748.
Tyagi R. and Ghose T. (1982). Biotechn. Bioeng. 24, 781–795.
Wilkinson K. and Rose I. (1979). J. Biol. Chem. 254, 12567–12572.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Galazzo, J.L., Shanks, J.V. & Bailey, J.E. Comparison of suspended and immobilized yeast metabolism using 31P nuclear magnetic resonance spectroscopy. Biotechnol Tech 1, 1–6 (1987). https://doi.org/10.1007/BF00156277
Issue Date:
DOI: https://doi.org/10.1007/BF00156277