Summary
Endo-xylanase from T. viride has been purified 4.2 fold by precipitation with a commercially available enteric polymer Eudragit S-100. Electrophoretic analysis also indicated removal of contaminant proteins. The enzyme could be recovered in more than 89% yield. The binding of the enzyme to the polymer was predominantly by electrostatic interaction.
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Gupta, M.N., Guoqiang, D., Kaul, R. et al. Purification of xylanase from Trichoderma viride by precipitation with an anionic polymer Eudragit S 100. Biotechnol Tech 8, 117–122 (1994). https://doi.org/10.1007/BF00152851
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DOI: https://doi.org/10.1007/BF00152851