Summary
Transformation-induced slow growth phenotype (SGP) in yeast is repressed in the presence of 2μm plasmids. A full 2μm-sequence-based recombinant plasmid (pJB502) was found to be more stable in a 2μm-free- [cir∘] strain of Saccharomyces cerevisiae than in a cir+ strain. This could not be attributed to differences in growth rate calculated from kinetic analysis of plasmid loss, but transformed [cir∘] isolates, which had lost the recombinant plasmid, exhibited varying degrees of SGP in batch culture. One of these isolates was outcompeted in chemostat culture by the recombinant-plasmid-containing strain, suggesting that improved plasmid maintenance can result from SGP in cir∘ hosts.
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O'Kennedy, R., Patching, J.W. Slow growth phenotype - A possible approach to improved plasmid maintenance in Saccharomyces cerevisiae . Biotechnol Lett 18, 713–718 (1996). https://doi.org/10.1007/BF00130771
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DOI: https://doi.org/10.1007/BF00130771