Summary
Crude yeast autolysate (10 g/l; 5.8 g solubles/l) supplemented with minerals and vitamins served as a nutrient supplement for ethanol production by the recombinant bacterium, Escherichia coli KO11. Ethanol production in this medium (46±1.7 g ethanol/l) was equivalent to that obtained using 5–15 g/l of purified, soluble, commercial protein hydrolysates (44–48 g ethanol/l). On site production of autolysates from spent yeast offers the potential for synergy between grain-based and lignocellulose-based ethanol plants.
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References
Amartey, S. and Jeffries, T. W. (1994). Biotechnol. Lett. 16,211–214.
Asghari, A., Bothast, R. J., Doran, J. B. and Ingram, L. O. (1996). J. Ind. Microbiol. 16,42–47.
Atkinson, B. and Mavituna, F. (1991). Biochemical Engineering and Biotechnology Handbook, second edition. Stockton Press, New York, NY.
Beall, D. S., Ingram, L. O., Ben-Bassat, A., Doran, J. B., Fowler, D. E., Hall, R. G. and Wood, B. E. (1992). Biotechnol. Lett. 14,857–862.
Beall, D. S., Ohta, K. and Ingram, L. O. (1991). Biotechnol. Bioeng. 38,296–303.
Brooks, T. A. and Ingram, L. O. (1995). Biotechnol. Progr. 11,619–625.
Ingram, L. O., Beall, D. S., Burchardt, G. F. H., Guimaraes, W. V., Ohta, K., Wood, B. E., and Shanmugam, K. T. (1995). Ethanol production by recombinant hosts. U.S. Patent No. 5,424,202.
Ingram, L. O., Conway, T., and Alterthum, F. (1991). Ethanol production by Escherichia coli strains co-expressing Zymomonas pdc and adh genes. U. S. Patent No. 5,000,000.
Ingram, L.O. and Doran, J. B. (1995). FEMS Microbiol. Rev. 16,235–241.
Jones, A. M. and Ingledew, W. M. (1994a). Bioresource Technol. 50,97–101.
Jones, A. M. and Ingledew, W. M. (1994b). Process Biochem. 29,483–488.
Katzen, R. and Fowler, D. E. (1994). Appl. Biochem. Biotechnol. 45/46,697–707.
Kollar, R., Sturdik, E. and Sajbidor, J. (1992). Food Biotechnol. 6,225–237.
Luria, S. E. and Delbruck, M. (1943). Genetics 28,491–511.
Ohta, K., Beall, D. S., Mejia, J. P., Shanmugam, K. T. and Ingram, L. O. (1991). Appl. Environ. Microbiol. 57,893–900.
Shah, M. M. and Cheryan, M. (1995). J. Indust. Microbiol. 15,4214–4218.
Thomas, K. C. and Ingledew, W. M. (1990). Appl. Environ. Microbiol. 56,2046–2050.
von Sivers, M., Zacchi, G., Olsson, L. and Hahn-Hagerdahl, B. (1994). Biotechnol. Prog. 10,555–560.
European Brewery Convention, Free amino nitrogen. In Analytica-EBC (4th edition). European Brewery Convention, Zurich, Switzerland, 1987, pp. E141–E142.
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York, S.W., Ingram, L.O. Ethanol production by recombinant Escherichia coli KO11 using crude yeast autolysate as a nutrient supplement. Biotechnol Lett 18, 683–688 (1996). https://doi.org/10.1007/BF00130766
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DOI: https://doi.org/10.1007/BF00130766