Summary
The sensitivity of twenty six microbial lipases towards acetaldehyde (an unavoidable by-product in lipase-catalysed acyl transfer reactions with vinyl esters) was investigated. The sensitivity of an individual enzyme strongly depends on its properties such as microbial source, molecular weight and relative lysine content. Whereas the majority of enzymes (from Pseudomonas, Rhizopus, Chromobacterium, Mucor and Candida antarctica sp.) proved to be remarkably stable, lipases from Candida rugosa and Geotrichum candidum lost most of their activity when exposed to acetaldehyde.
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Ader, U., Breitgoff, D., Klein, P., Laumen, K. E. and Schneider, M. P. (1989) Tetrahedron Lett. 30, 1793–1796.
Battistel, E., Bianchi, D., Cesti, P. and Pina, C. (1991) Biotechnol. Bioeng. 38, 659–664.
Berger, B. and Faber, K. (1991) J. Chem. Soc., Chem. Commun. 1198–1200.
Carrea, G., Ottolina, G. and Riva, S. (1995) Trends Biotechnol. 13, 63–70.
Colton, I. J., Ahmed, S. N., and Kazlauskas, R. J. (1995) J. Org. Chem. 60, 212–217.
Cygler, M., Schrag, J. D., Sussman, J. L., Harel, M., Silman, I., Gentry, M. K. and Doctor, B. P. (1993) Protein Sci. 2, 366–382.
Degueil-Castaing, M., De Jeso, B.; Drouillard, S. and Maillard, B. (1987) Tetrahedron Lett. 28, 953–954.
Faber, K. and Riva, S. (1992) Synthesis 895–910.
Holla, E. W. (1989) Angew. Chem., Int. Ed. Engl. 28, 220–221.
Holla, E. W. (1990) J. Carbohydr. Chem. 9, 113–119.
Kaga, H., Siegmund, B., Neufellner, E., Faber, K. and Paltauf, F. (1994) Biotechnol. Tech. 8, 369–374.
Marks, T. A., Quinn, J. G., Sampugna, J. and Jensen, R. G. (1968) Lipids 3, 143–146.
Mitsuda, S. and Nabeshima, S. (1991) Recl. Trav. Chim. Pays-Bas 110, 151–154.
Santaniello, E., Ferraboschi, P. and Grisenti, P. (1993) Enzyme Microb. Technol. 15, 367–382.
Svendsen, A. (1994) Sequence comparisons within the lipase family. In: Lipases, Woolley, P. and Petersen, S. B. (eds.), Cambridge University Press, pp. 1–21.
Wang, Y.-F. and Wong, C.-H. (1988) J. Org. Chem. 53, 3127–3129.
Winkler, U. K. and Stuckmann, M. (1979) J. Bacteriol. 138, 663–670.
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Weber, H.K., Stecher, H. & Faber, K. Sensitivity of microbial lipases to acetaldehyde formed by acyl-transfer reactions from vinyl esters. Biotechnol Lett 17, 803–808 (1995). https://doi.org/10.1007/BF00129008
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DOI: https://doi.org/10.1007/BF00129008