Summary
For the secretion of human lipocortin-1 (LC-1) in yeast, a expression and secretion vector was constructed by using the promoter and signal sequence of glucoamylase gene (STA1) of Saccharomyces diastaticus. After the cDNA of human LC-1 was ligated with the secretion vector, the resulting hybrid plasmid was transformed into S. diastaticus. When the recombinant S. diastaticus was cultivated in YPD medium, LC-1 was expressed and secreted into the extracellular medium, yielding LC-1 protein at a concentration of 2.5 μg/mL.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Castanon, M.J., Spevak, W., Adolf, G.R., Chlebowicz-Sledziewska, E, and Sledziewski, A. (1988). Gene. 66. 223–234.
De Nobel, J.G. and Barnett, J.A. (1991). Yeast. 7. 313–323.
Flower, R.J. (1988). Br. J. Pharmacol. 94. 987–1015.
Ito, H., Fukuda, Y., Murata, K., and Kimura, A. (1983). J. Bacteriol. 153. 163–168.
Jigami, Y., Muraki, M., Harada, N., and Tanaka, H. (1986). Gene. 43. 273–279.
Julius, D., Brake, A., Blair, L., Kunisawa, R., and Thorner, J. (1984). Cell. 37. 1075–1089.
Huh, K.R., Park, S.H., Kang, S. M., Song, I.S., L, H.Y., and Na, D.S. (1990). Korean Biochem. J. 23. 459–464.
Laemmli, U.K. (1970). Nature. 227. 680–685.
Miyajima, A., Bond, M.W., Otsu, K., Arai, K., and Arai, N. (1986). Gene. 37. 155–161.
Moir, D.T. and Dumais, D.R. (1987). Gene. 56. 209–217.
Nakamura, M., Matsumoto, T., Noguchi, M., Yamashita, I., and Noma, M. (1990). Gene. 89. 231–237.
Nakata, T., Sobue, K., and Hirokawa, N. (1990). J. Cell. Biol. 110. 13–25.
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989). Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Smith, R.A., Duncan, M.J., and Moir, D.T. (1985). Science. 229. 1219–1224.
Wallner, B.P., Mattaliano, R.J., Hession, C., Cate, R.L., Tizard, R., Sinclair, L.K., Foeller, C., Chow, E.P., Browning, J.L., Ramachandran, K.L., and Pepinsky, R.B. (1986). Nature. 320. 77–81.
Yamashita, I., Suzuki, K., and Fukui, S. (1985). J. Bacteriol. 161. 567–573.
Yamashita. I. (1989). Agric. Biol. Chem. 53. 483–489.
Zsebo, K.M., Lu, H.-S., Fieschko, J.C., Goldstein, L., Davis, J., Duker, K., Suggs, S.V., Lai, P.H., and Bitter, G.A. (1986). J. Biol. Chem. 261. 5858–5865.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nam, S.W., Kim, B.M., Chung, B.H. et al. Expression and secretion of human lipocortin-1 by promoter and signal sequence of STA1 from Saccharomyces diastaticus . Biotechnol Lett 16, 897–902 (1994). https://doi.org/10.1007/BF00128621
Issue Date:
DOI: https://doi.org/10.1007/BF00128621