Summary
An improved Chromatographic technique was developed to monitor the acetone/butanol fermentation ‘on-line’. Reduction in column length and diameter, use of a compromise oven temperature for multicomponent systems and smaller headspace gas aliquots permitted the completion of chromatograms within 3.5 minutes. Transient solvent concentrations were sensed within 2.5 minutes, and the chromatographic response was linear to 110 g/L ethanol and 20 g/L acetone and butanol concentrations.
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Comberbach, D.M., Scharer, J.M. & Moo-Young, M. An improved chromatographic procedure for the rapid determination of volatile solvents via the headspace gas. Biotechnol Lett 6, 91–96 (1984). https://doi.org/10.1007/BF00127296
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DOI: https://doi.org/10.1007/BF00127296