Abstract
Cultured Rauwolfia serpentina calluses consisted of cell colonies that had different fluorescences under 365 nm UV-light. Fluorescence was divisible into two main color categories, yellow-green and blue-white. Two cell strains of different fluorescence were selected from calluses cultured in the dark, by the fluorescence assay. Even after the selected strains had been transferred to liquid medium, they maintained similar fluorescence. HPLC analysis showed that the yellow-green fluorescent strains produced much reserpine, whereas the blue-white strains produced much 3,4,5-trimethoxy benzoic acid. A combination of 10μM NAA and 10μM BA enhanced production of reserpine in the yellow-green fluorescent cell strains.
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Yamamoto, O., Yamada, Y. Selection of a reserpine-producing cell strain using UV-light and optimization of reserpine production in the selected cell strain. Plant Cell Tiss Organ Cult 8, 125–133 (1987). https://doi.org/10.1007/BF00043149
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DOI: https://doi.org/10.1007/BF00043149