Abstract
The in vitro proliferation of Vitis vinifera L. cv. Liemberger in a liquid medium was compared in 125 and 250 ml Erlenmeyer flasks and in 473 ml (pint) Mason jars. After 6 weeks of culture the jars yielded a significantly greater number of shoots 3 mm or longer than the flasks. Jars yielded the greatest number of shoots 7 mm or longer, followed by 250 ml, then 125 ml flasks. The mean length of shoots in the 250 ml flasks was significantly greater than that of shoots in 125 ml flasks. The final mean fresh weight of the cultures in jars was significantly less than that of the cultures in flasks. Thus the size of vessel used influenced the in vitro proliferation of grapevines in liquid culture.
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Monette, P.L. Influence of size of culture vessel on in vitro proliferation of grape in a liquid medium. Plant Cell Tiss Organ Cult 2, 327–332 (1983). https://doi.org/10.1007/BF00039879
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DOI: https://doi.org/10.1007/BF00039879