Abstract
Suspension cultures of Coleus blumei were characterized with respect to growth and rosmarinic acid formation in media with different sugars and various sugar concentrations. Sucrose is the sugar with the highest stimulating effect on growth and rosmarinic acid accumulation, followed by glucose and fructose. The sugar alcohol mannitol cannot be metabolized by the plant cells. Sucrose is cleaved into glucose and fructose by the Coleus cells. Sucrose concentrations from 1 to 5% have an increasing positive effect on growth and rosmarinic acid synthesis in the cell cultures with a maximum rosmarinic acid content of 12% of the dry weight in medium with 5% sucrose; in medium with 6% sucrose rosmarinic acid accumulation obviously did not reach its highest level in the culture period of 14 days. A very high yield of rosmarinic acid (2 mg ml-1 suspension) could also be achieved by maintaining a sucrose concentration of 2% during the whole culture period. The start of rosmarinic acid synthesis by the cell cultures seems to be regulated by the growth limitation when a nutrient, e.g. phosphate is depleted from the medium. The rate of rosmarinic acid accumulation is related to the amount of carbon left in the medium when growth ceases.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- RA:
-
rosmarinic acid
References
De Eknamkul W & Ellis BE (1988) Rosmarinic acid: Production in plant cell cultures. In: Bajaj YPS (Ed) Biotechnology in Agriculture and Forestry. Medicinal and Aromatic Plants I, Vol 4 (pp 310–329). Springer Verlag, Berlin, Heidelberg
Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158
Gomorri G (1942) A modification of the colorimetric phosphorus determination for use with the photoelectric colorimeter. J. Lab. Clin. Med. 27: 955
Häusler E, Petersen M & Alfermann AW (1991) Hydroxyphenylpyruvate reductase from cell suspension cultures of Coleus blumei Benth. Z. Naturforsch 46c: 371–376
Hippolyte I, Marin B, Baccou JC & Jonard R (1990) Influence du milieu d'entretien et de la concentration en saccharose sur la production d'acide rosmarinique par des suspensions cellulaires de Sauge (Salvia officinalis L). C. R. Acad. Sci. Paris, Série III, 313: 365–371
Hippolyte I, Marin B, Baccou JC & Jonard R (1992) Growth and rosmarinic acid production in cell suspension cultures of Salvia officinalis L. Plant Cell Rep 11: 109–112
Petersen MS (1991) Characterization of rosmarinic acid synthase from cell cultures of Coleus blumei. Phytochemistry 30: 2877–2881
Petersen M & Alfermann AW (1988) Two new enzymes of rosmarinic acid biosynthesis from cell cultures of Coleus blumei: Hydroxyphenylpyruvate reductase and rosmarinic acid synthase. Z. Naturforsch. 43c: 501–504
Petersen M, Häusler E, Karwatzki B & Meinhard J (1993) Proposed biosynthetic pathway for rosmarinic acid in cell suspension cultures of Coleus blumei. Planta 189: 10–14
Razzaque A & Ellis BE (1977) Rosmarinic acid production in Coleus cell cultures. Planta 137: 287–291
Schiel O, Jarchow-Redecker K, Piehl GW, Lehmann J & Berlin J (1984) Increased formation of cinnamoyl putrescines by fedbatch fermentation of cell suspension cultures of Nicotiana tabacum. Plant Cell Rep. 3: 18–20
Ulbrich B, Wiesner W & Arens H (1985) Large-scale production of rosmarinic acid from plant cell cultures of Coleus blumei Benth. In: Neumann KH, Barz W & Reinhard E (Eds) Primary and Secondary Metabolism of Plant Cell Cultures (pp 293–303). Springer Verlag, Berlin, Heidelberg
Wray V, Schiel O & Berlin J (1983) High field phosphorous-31 nuclear magnetic resonance investigation of the phosphate metabolites in cell suspension cultures of Nicotiana tabacum. Z. Pflanzenphysiol. 112: 215–220
Yokoyama M & Yanagi M (1991) High-level production of arbutin by biotransformation. In: Komamine A, Misawa M & DiCosmo F (Eds) Plant Cell Culture in Japan (pp 79–91). CMC Co., Ltd., Tokyo
Zenk MH, El-Shagi H & Ulbrich B (1977) Production of rosmarinic acid by cell-suspension cultures of Coleus blumei. Naturwissenschaften 64: 585–586
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gertlowski, C., Petersen, M. Influence of the carbon source on growth and rosmarinic acid production in suspension cultures of Coleus blumei . Plant Cell Tiss Organ Cult 34, 183–190 (1993). https://doi.org/10.1007/BF00036100
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00036100