Abstract
An increase of the vapor pressure deficit (VPD) of the growth room atmosphere (from 600 Pa up to 2000 Pa) induced a variation in the air VPD inside the vessels used for rose micropropagation.
During the photoperiod, the in vitro plants lost water by evaporation. During the night period, depending upon the VPD of the growth room, plants could take water from the vessel atmosphere.
According to the intensity of the transpiration, large changes in the growth and morphology were observed: decrease in multiplication rate, modification of leaf colour and area, reduction of the elongation and changes of the level of axillary buds which grew.
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Bateson JM, Grout BWW & Lane S (1987) The influence of containers dimensions on the multiplication rate of regenerating plant cell cultures. Symposium Florizel, Plant micropropagation in Horticultural industries, Belgium
Brainerd KE & Fuchigami H (1982) Stomatal functioning of in vitro and greenhouse apple leaves in darkness, mannitol, ABA and CO2. J. Exp. Bot. 33: 388–392
Conner LM & Conner AJ (1984) Comparative water loss from leaves of Solanium laciniatum plants cultured in vitro and in vivo. Plant Sci. Lett. 36: 241–246
Debergh P, Harbaoui Y & Lemeur R (1981) Mass propagation of Globe Artichoke (Cyanara scolymus): evaluation of different hypothesis to overcome vitrification with special references to water potential. Physiol. Plant. 53: 181–187
Kosai T, Fujiwara K & Watanabe I (1986) Fundamental studies on environments in plant tissue culture vessels (1). Relations between the culture medium composition and the water potential of liquid culture media. J. Agr. Met. 42: 1–6
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Sallanon H & Coudret A (1990) Flux d'eau entre vitroplants et atmosphère en micropropagation. C.R. Acad. Sci. 310: 607–613
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Sallanon, H., Maziere, Y. Influence of growth room and vessel humidity on the in vitro development of rose plants. Plant Cell Tiss Organ Cult 30, 121–125 (1992). https://doi.org/10.1007/BF00034305
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DOI: https://doi.org/10.1007/BF00034305