Abstract
Bean (Phaseolus vulgaris L.) mature embryos were transformed using biolistic methods with a plasmid containing 2S albumin and β-glucuronidase structural sequences, both under the control of the 35S CaMV promoter. We have shown that chimaeric tissues could be obtained and that both structural sequences were expressed to similar levels.
Article PDF
Avoid common mistakes on your manuscript.
References
Gander ES, Holmstroen K-O, DePaiva GR, DeCastro LAB, Carneiro M, Grossi de Sa M-F: Isolation, characterisation and expression of a gene coding for a 2S albumin from Bertholletia excelsa (Brazil nut). Plant Mol Biol 16: 437–448 (1991).
Grossi de Sa M-F, Martins de Sa C, Harper F, Coux O, Akhayat O, Pal JK, Florentin Y, Scherrer K: Cytolocalization of prosomes as a function of differentiation. J Cell Sci 89: 15: 151–165 (1988).
Jefferson RA, Kavanagh TA, Bevan MW: GUS fusions: Beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907 (1987).
Klein TM, Wolf ED, Wu R, Sanford JC: High-velocity microprojectiles for delivering nucleic acids into living cells. Nature 327: 70–73 (1987).
Murashige T, Skoog F: A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497 (1962).
Rech EL, Vainstein MH, Davey MR: An electrical particle acceleration gun for gene transfer into cells. Technique, in press (1991).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Aragao, F.J.L., Grossi de Sa, M.F., Almeida, E.R. et al. Particle bombardment-mediated transient expression of a Brazil nut methionine-rich albumin in bean (Phaseolus vulgaris L.). Plant Mol Biol 20, 357–359 (1992). https://doi.org/10.1007/BF00014508
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00014508