Abstract
The NTA/EDTA fractionation of sediment-bound, inorganic phosphate was improved and made more effective. The iron-bound phosphate is now extracted with Ca-EDTA plus dithionite and followed by an extraction of calcium-bound phosphate by Nat-EDTA at pH = 4.5. A comparison is made with two other sequential extraction procedures: the SEDEX and the Hieltjes & Lijklema extractions. The EDTA extractions have an advantage over the other two methods.
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Abbreviations
- o-P =:
-
ortho phosphate
- ASOP=:
-
Acid Soluble Organic Phosphate
- TRIS-buffer=:
-
tris (hydroximethyl)-amino methane
- NTA=:
-
Nitrilo triacetic acid (or its salts)
- M =:
-
mol dm 3
- EDTA=:
-
Ethylene dinitrilo tetraacetic acid(or its salts)
- Fe(OOH)≈P =:
-
iron-bound phosphate
- CaCO3 ≈P =:
-
calcium-bound phosphate
- NaOH-Pextr =:
-
phosphate extracted with NaOH (etc.)
References
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Golterman, H.L. Fractionation of sediment phosphate with chelating compounds: a simplification, and comparison with other methods. Hydrobiologia 335, 87–95 (1996). https://doi.org/10.1007/BF00013687
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DOI: https://doi.org/10.1007/BF00013687