Résumé
Un net changement de la stéroïdogenèse, passant de la production de testostérone à celle de 17α-hydroxy-progestérone, apparait dans les cellules ovariennes de la thèque des salmonidés, juste avant la maturation ovocytaire. Ce changement est un prérequis pour la production de 17α,20β-dihydroxy-4-pregnene-3-one (stéroïde inducteur de la maturation chez les salmonidés), par les cellules de la granulosa, durant la maturation ovocytaire. Le cytochrome P-450 portant l'activité 17α-hydroxylase/17,20-lyase (P-45017α) et la 3β-hydroxystéroïde déshydrogénase/Δ5−4-isomérase (3β-HSD) sont les deux enzymes stéroïdogènes majeures impliquées dans la synthèse de la 17α-hydroxyprogestérone et de la testostérone. En utilisant des sondes ADNc de mammifère, nous avons isolé et caractérisé un ADNc complet codant pour ces deux enzymes, à partir d'une librairie d'ADNc de cellules ovariennes thèquales de truite arc-en-ciel (Oncorhynchus mykiss). Le clonage d'un ADNc, de 2,4 kilobase, codant pour le P-45017α, et l'expression transitoire de ce clone dans des cellules non stéroïdogènes de tumeur de rein, COS-1, de singe, ont été récemment rapportés (Sakai et al. 1992). Nous avons isolé un ADNc de 1,4 kilobase qui s'hybride avec l'ADNc mammalien de la 3β-HSD. L'expression de cet ADNc, dans les cellules COS-1, conduit à la production d'une enzyme capable de convertir la déhydroépiandrostérone en androsténédione. Une discussion est faite sur l'évolution des activités enzymatiques et de l'expression de la P-45017α et de la 3β-HSD, dans l'ovaire de truite arc-en-ciel, en rapport avec la modification de la stéroïdogenèse apparaissant dans les enveloppes du follicule ovarien.
Abstract
A distinct shift in steroidogenesis from testosterone to 17α-hydroxyprogesterone occurs in the salmonid ovarian thecal cell layers immediately prior to oocyte maturation, and is a prerequisite for the production of 17α,20β-dihydroxy-4-pregnen-3-one (maturation-inducing hormone of salmonid fishes) by granulosa cells during oocyte maturation. 17α-Hydroxylase/17,20-lyase cytochrome P-450 (P-45017α) and 3β-hydroxysteroid dehydrogenase/Δ5−4-isomerase (3β-HSD) are the two major steroidogenic enzymes involved in the production of 17α-hydroxyprogesterone and testosterone. Using mammalian cDNA probes, we isolated and characterized full-length cDNAs encoding these two enzymes from a rainbow trout (Oncorhynchus mykiss) ovarian thecal cell cDNA library. The cloning of 2.4-kilobase cDNA encoding P-45017α and transient expression of this clone in nonsteroidogenic monkey kidney tumor COS-1 cells have recently been reported (Sakai et al. 1992). We have isolated a 1.4-kilobase cDNA which is hybridized to the mammalian 3β-HSD cDNAs. Expression of this cDNA in COS-1 cells led to the production of an enzyme which is capable of converting dehydroepiandrosterone to androstenedione. In this study, enzymatic activities and expression of rainbow trout ovarian P-45017α and 3β-HSD are discussed in relation of the steroidogenic shift occurring in the ovarian follicle layers.
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Sakai, N., Tanaka, M., Takahashi, M. et al. Isolation and expression of rainbow trout (Oncorhynchus mykiss) ovarian cDNA encoding 3β-hydroxysteroid dehydrogenase/Δ5−4-isomerase. Fish Physiol Biochem 11, 273–279 (1993). https://doi.org/10.1007/BF00004575
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DOI: https://doi.org/10.1007/BF00004575