Abstract
The effects that ultraviolet rays elicit on collagen synthesis and degradation are the most common causes of wrinkle formation and photo-aging in skin. The objectives of this study were to evaluate the effects of Angelica acutiloba root ethanol extract (AAEE) to promote collagen synthesis and inhibit collagen degradation in human dermal fibroblasts. By examining total polyphenol and flavonoid contents, electron donating ability, radical scavenging activity, and superoxide dismutase-like activity, we found that AAEE exhibited fairly good antioxidant activity. Treatment with AAEE significantly increased type I procollagen production by cultured fibroblasts, as well as reduced ultraviolet-induced matrix metalloproteinase-1 (MMP-1) expression and MMP-2 activity in a dose-dependent manner (p < 0.05). In addition, AAEE significantly increased TIMP-1 mRNA expression (p < 0.05), although without an associated dose-dependent increase in TIMP-1 protein expression. In summary, we suggest that AAEE may be a potentially effective agent for the prevention or alleviation of skin-wrinkle formation induced by ultraviolet rays.
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Tzaphlidou, M. (2004) The role of collagen and elastin in aged skin: an image processing approach. Micron, 35, 173–177.
Song, K.C., Chang, T.S., Lee, H.J., Kim, J.H., Park, J.H. and Hwang, G.S. (2012) Processed Panax ginseng, sun ginseng increases type I collagen by regulating MMP-1 and TIMP-1 expression in human dermal fibroblasts. J. Ginseng Res., 36, 61–67.
Varani, J., Dame, M.K., Rittie, L., Fligiel, S.E., Kang, S., Fisher, G.J. and Voorhees, J.J. (2006) Decreased collagen production in chronologically aged skin: roles of age-dependent alteration in fibroblast function and detective mechanical stimulation. Am. J. Pathol., 168, 1861–1868.
Honda, A., Abe, R., Makino, T., Norisugi, O., Fujita, Y., Watanabe, H., Nishihira, J., Iwakura, Y., Yamagishi, S.I., Shimizu, H. and Shimizu, T. (2008) Interleukin-1β and macrophage migration inhibitory factor (MIF) in dermal fibroblasts mediate UVA-induced matrix metalloproteinase-1expression. J. Dermatol. Sci., 49, 63–72.
Brew, K., Deendayal, D. and Nagase, H. (2000) Tissue inhibitors of metalloproteinases: evolution, structure and function. Biochim. Biophys. Acta, 1477, 267–283.
Dasu, M.R., Barrow, R.E., Spies, M. and Herndon, D.N. (2003) Matrix metalloproteinase expression in cytokine stimulated human dermal fibroblasts. Burns, 29, 527–531.
Tokumaru, Y., Fujii, M., Otani, Y., Kameyama, K., Imanishi, Y., Igarashi, N. and Kanzaki, J. (2000) Activation of matrix metalloproteinase-2 in head and neck squamous cell carcinoma: studies of clinical samples and in vitro cell lines co-cultured with fibroblasts. Cancer Lett., 150, 15–21.
Godeau, G. and Hornebeck, W. (1988) Morphometric analysis of the degradation of human skin elastic fibres by human leukocyte elastase (EC 3-4-21-37) and human skin fibroblast elastase (EC 3-4-24). Pathol. Biol., 36, 1133–1138.
Kim, M.S., Lee, S.R., Rho, H.S., Kim, D.H., Chang, I.S. and Chung, J.H. (2005) The effects of a novel synthetic retinoid, seletinoid G, on the expression of extracellular matrix proteins in aged human skin in vivo. Clin. Chim. Acta, 362, 161–169.
Ha, B.G., Park, M.A. Lee, C.M. and Kim, Y.C. (2015) Antiox-idant activity and anti-wrinkle effects of Aceriphyllum rossii leaf ethanol extract. Toxicol. Res., 31, 363–369.
Kim, S.E., Lee, C.M. and Kim, Y.C. (2016) Anti-wrinkle efficacy of Oenothera laciniata methanol extract in human dermal fibroblasts. J. Invest. Cosmotol., 12, 197–203.
Sheng, Y.X., Li, L., Wang, Q., Guo, H.Z. and Guo, D.A. (2005) Simultaneous determination of gallic acid, albiflorin, paeoniflorin, ferulic acid and benzoic acid in Si-Wu decoction by high-performance liquid chromatography DAD method. J. Pharm. Biomed. Anal., 37, 805–810.
Wen, K.C., Huang, C.Y. and Lu, F.L. (1993) Determination of baicalin and puerarin in traditional Chinese medicinal preparations by high-performance liquid chromatography. J. Chromatogr. A, 631, 241–250.
Namba, T. (1993) Angelicae Radix, Hoikusha, Osaka, pp. 58–61.
Kim, A.R., Lee, J.J. and Lee, M.Y. (2009) Antioxidative effect of Angelica acutiloba Kitagawa ethanol extract. J. Life Sci., 19, 117–122.
Folin, O. and Denis, W. (1912) On phosphotungstic-phospho-molybdic compounds as color reagents. J. Biol. Chem., 12, 239–243.
Davis, R., Massey, R.C. and McWeeny, D.J. (1980) The catalysis of the N-nitrosation of secondary amines by nitrosophenols. Food Chem., 6, 115–122.
Blois, M.S. (1958) Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199–1200.
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. and Rice-Evans, C. (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med., 26, 1231–1237.
Marklund, S. and Marklund, G. (1974) Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem., 47, 469–474.
Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193, 265–275.
Joo, S.S., Park, D., Shin, S., Jeon, J.H., Kim, T.K., Choi, Y.J., Lee, S.H., Kim, J.S., Park, S.K., Hwang, B.Y., Lee, D.I. and Kim, Y.B. (2010) Anti-allergic effects and mechanisms of action of the ethanolic extract of Angelica gigas in dinitrofluo-robenzene-induced inflammation models. Environ. Toxicol. Pharmacol., 30, 127–133.
Wang, N.L., Kiyohara, H., Sakurai, M.H. and Yamada, H. (1999) Antigenic epitope for polyclonal antibody against a complement activating pectin from the roots of Angelica acutiloba Kitagawa. Carbohydr. Polym., 39, 257–264.
Bickers, D.R. and Athar, M. (2006) Oxidative stress in the pathogenesis of skin disease. J. Invest. Dermatol., 126, 2565–2575.
Scharffetter-Kochanek, K., Brenneisen, P., Wenk, J., Herrmann, G., Ma, W., Kuhr, L., Meewes, C. and Wlaschek, M. (2000) Photoaging of the skin from phenotype to mechanisms. Exp. Gerontol., 35, 307–316.
Miao, Y.P., Wen, R., Aoshima, H. and Zhou, P.G. (2004) Synthesis and antioxidative activity of 2-substituted phenyl-5-(3'-indolyl)-oxazole derivatives. Yao Xue Xue Bao, 39, 37–40.
Park, S.J., Yoon, J.H., Kim, Y.E., Yoon, Y.B. and Kim, J.D. (2011) In vitro antioxidant activity of the aqueous of Angelicae gigas Nakai leaves. Korean J. Food Preserv., 18, 817–823.
Mimura, Y., Ihn, H., Jinnin, M., Asano, Y., Yamane, K. and Tamaki, K. (2006) Epidermal growth factor affects the synthesis and degradation of type I collagen in cultured human dermal fibroblasts. Matrix Biol., 25, 202–212.
Pasquali-Ronchetti, I. and Baccarani-Contri, M. (1997) Elastic fiber during development and aging. Microsc. Res. Tech., 38, 428–435.
Bruckner-Tuderman, L., Schnyder, U.W., Winterhalter, K.H. and Bruckner, P. (1987) Tissue form of type VII collagen from human skin and dermal fibroblasts in culture. Eur. J. Biochem., 165, 607–611.
Di Lullo, G.A., Sweeney, S.M., Körkkö, J., Ala-Kokko, L. and San Antonio, J.D. (2002) Mapping the ligand-binding sites and disease-associated mutations on the most abundant protein in the human, type I collagen. J. Biol. Chem., 277, 4223–4231.
Talwar, H.S., Griffiths, C.E., Fisher, G.J., Hamilton, T.A. and Voorhees, J.J. (1995) Reduced type I and type III procollagens in photodamaged adult human skin. J. Invest. Dermatol., 105, 285–290.
Gordon, M.K. and Hahn, R.A. (2010) Collagens. Cell Tissue Res., 339, 247–257.
Lee, J.H., Lee, S.N., Kim, M.G., Kim, M.H., Kim, H.J., Jo, H.J. and Leem, K.H. (2011) Effects of Angelica gigantis Radix extracts on the collagenase activity and procollagen synthesis in HS68 human fibroblasts and tyrosinase activity. Korea J. Herbol., 26, 29–33.
Borkakoti, N. (1998) Matrix metalloproteases: variations on a theme. Prog. Biophys. Mol. Biol., 70, 73–94.
Herman, M.P., Sukhova, G.K., Kisiel, W., Foster, D., Kehry, M.R., Libby, P. and Schönbeck, U. (2001) Tissue factor pathway inhibitor-2 is a novel inhibitor of matrix metalloprotein-ases with implications for atherosclerosis. J. Clin. Invest., 107, 1117–1126.
Sternlicht, M.D. and Werb, Z. (2001) How matrix metalloproteinases regulate cell behavior. Annu. Rev. Cell Dev. Biol., 17, 463–516.
Hwang, B.M., Noh, E.M., Kim, J.S., Kim, J.M., Hwang, J.K., Kim, H.K., Kang, J.S., Kim, D.S., Chae, H.J., You, Y.O., Kwon, K.B. and Lee, Y.R. (2013) Decursin inhibits UVB-induced MMP expression in human dermal fibroblasts via regulation of nuclear factor-κB. Int. J. Mol. Med., 31, 477–483.
Kähäri, V.M. and Saarialho-Kere, U. (1997) Matrix metallo-proteinases in skin. Exp. Dermatol., 6, 199–213.
Aimes, R.T. and Quigley, J.P. (1995) Matrix metalloprotein-ase-2 is an interstitial collagenase. Inhibitor-free enzyme catalyzes the cleavage of collagen fibrils and soluble native type I collagen generating the specific 3/4- and 1/4-length fragments. J. Biol. Chem., 270, 5872–5876.
You, M.A., Song, Y.K., Jang, H., Kim, D.M. and Byun, S.Y. (2011) Profiling of skin anti-aging related proteins in human dermal fibroblasts by decursin in Angelica gigas Nakai. Korean J. Chem. Eng., 28, 880–885.
Hwang, B.M., Noh, E.M., Kim, J.S., Kim, J.M., Hwang, J.K., Kim, H.K., Kang, J.S., Kim, D.S., Chae, H.J., You, Y.O., Kwon, K.B. and Lee, Y.R. (2013) Decursin inhibits UVB-induced MMP expression in human dermal fibroblasts via regulation of nuclear factor-κB. Int. J. Mol. Med., 31, 477–483.
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Park, M.A., Sim, M.J. & Kim, Y.C. Anti-Photoaging Effects of Angelica acutiloba Root Ethanol Extract in Human Dermal Fibroblasts. Toxicol Res. 33, 125–134 (2017). https://doi.org/10.5487/TR.2017.33.2.125
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DOI: https://doi.org/10.5487/TR.2017.33.2.125