Abstract
Fluorescent in situ hybridization (FISH) and PCR were used for analysis of phylogenetic structure of anaerobic sulfate-reducing bacterial communities in oxygen-containing upper water layers of meromictic basins: the Black Sea and the Gdansk Deep of the Baltic Sea. In the Black Sea (continental slope at depths 30–70 m), cells of sulfate-reducing bacteria (SRB) hybridizing with 16S rRNA-specific FISH-probes for Desulfotomaculum, Desulfobacter, and Desulfovibrio genera were revealed, whereas Desulfomicrobium-related bacteria were prevalent in the chemocline zone at a 150-m depth. Besides Desulfotomaculum (SRB subgroup 1), Desulfobacter (SRB subgroup 4), and Desulfovibrio-Desulfomicrobium (SRB subgroup 6), nested PCR with the use of 16S rRNA gene-specific primers detected the presence of Desulfococcus–Desulfonema–Desulfosarcina (SRB subgroup 5) in the oxygen-containing water column of the Black and Baltic seas. Active enrichment SRB culture that contained bacterium Desulfosporosinus sp. as a major component was obtained from the Black Sea water sample collected at a 70-m depth.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Murray, J.W., Jannash, H.W., Honjo, S., Anderson, R.F., Reeburgh, W.S., Top, Z., Friederich, G.E., Codispoti, L.A., and Izdar, E., Unexpected changes in the oxic/anoxic interface in the Black Sea, Nature, 1989, vol. 338, no. 6214, pp. 411–413.
Bryukhanov, A.L., Korneeva, V.A., Kanapatskii, T.A., Zakharova, E.E., Men’ko, E.V., Rusanov, I.I., and Pimenov, N.V., Investigation of the sulfate-reducing bacterial community in the aerobic water and chemocline zone of the Black Sea by the FISH technique, Microbiology, 2011, vol. 80, no. 1, pp. 108–116.
Kot-Wasik, A., Zukowska, B., Dabrowska, D., Debska, J., Pacyna, J., and Namiesnik, J., Physical, chemical, and biological changes in the Gulf of Gdansk ecosystem (southern Baltic Sea), Rev. Environ. Contam. Toxicol., 2003, vol. 179, no. 1, pp. 1–36.
Pimenov, N.V., Rusanov, I.I., Yusupov, S.K., Fridrich, J., Lein, A.Yu., Wehrli, B., and Ivanov, M.V., Microbial processes at the aerobic-anaerobic interface in the deep-water zone of the Black Sea, Microbiology, 2000, vol. 69, no. 4, pp. 527–540.
Brioukhanov, A., Pieulle, L., and Dolla, A., Antioxidative defense systems of anaerobic sulfate-reducing microorganisms, in Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, vol. 1. Microbiology Book Series, Mendez-Vilas, A., Ed., Badajoz: Formatex Research Center, 2010, pp. 148–159.
de Rezende, J.R., Kjeldsen, K.U., Hubert, C.R., Finster, K., Loy, A., and Jorgensen, B.B., Dispersal of thermophilic Desulfotomaculum endospores into Baltic Sea sediments over thousands of years, ISME J., 2013, vol. 7, no. 1, pp. 72–84.
Daly, K., Sharp, R.J., and McCarthy, A.J., Development of oligonucleotide probes and PCR primers for detecting phylogenetic subgroups of sulfate-reducing bacteria, Microbiology-SGM, 2000, vol. 146, no. 7, pp. 1693–1705.
Amann, R.I., Zarda, B., Stahl, D.A., and Schleifer, K.H., Identification of individual prokaryotic cells by using enzyme-labeled, rRNA-targeted oligonucleotide probes, Appl. Environ. Microbiol., 1992, vol. 58, no. 9, pp. 3007–3011.
Devereux, R., Kane, M.D., Winfrey, J., and Stahl, D.A., Genusand group-specific hybridization probes for determinative and environmental studies of sulfatereducing bacteria, Syst. Appl. Microbiol., 1992, vol. 15, no. 4, pp. 601–609.
Hristova, K.R., Mau, M., Zheng, D., Aminov, R.I., Mackie, R.I., Gaskins, H.R., and Raskin, L., Desulfotomacullum genusand subgenus-specific 16S rRNA hybridization probes for environmental studies, Environ. Microbiol., 2000, vol. 2, no. 2, pp. 143–159.
Lucker, S., Steger, D., Kjeldsen, K.U., MacGregor, B.J., Wagner, M., and Loy, A., Improved 16S rRNA-targeted probe set for analysis of sulfate-reducing bacteria by fluorescence in situ hybridization, J. Microbiol. Methods, 2007, vol. 69, no. 3, pp. 523–528.
Edwards, U., Rogall, T., Blocker, H., Emde, M., and Bottger, E.C., Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA, Nucleic Acid Res., 1989, vol. 17, no. 19, pp. 7843–7853.
Geets, J., Borremans, B., Diels, L., Springael, D., Vangronsveld, J., van der Lelie, D., and Vanbroekhoven, K., DsrB gene-based DGGE for community and diversity surveys of sulfate-reducing bacteria, J. Microbiol. Methods, 2006, vol. 66, no. 2, pp. 194–205.
Widdel, F. and Back, F., The genus Desulfotomaculum, in The Prokaryotes, 3rd ed., vol. 4, Dworkin, M., Falkow, S., Rosenberg, E., Schleifer K.H., Stackebrandt, E., Eds., N.Y.: Springer, 1992, pp. 787–794.
Truper, H.G. and Schlegel, H.G., Sulfur metabolism in Thiorhodaceae. I. Quantitative measurements on growing cells of Chromatium okenii, Antonie van Leeuwenhoek, 1964, vol. 30, no. 1, pp. 225–238.
Muyzer, G., Brinkhoff, T., Nubel, U., Santegoeds, C., Schafer, H., and Wawer, C., Denaturing gradient gel electrophoresis (DGGE) in microbial ecology, in Molecular Microbial Ecology Manual, Akkermans, A.D.L., van Elsas, J.D., and de Bruijn, F.J., Eds., Dordrecht: Kluwer Academic Publishers, 1997, pp. 1–23.
Vetriani, C., Tran, H.V., and Kerkhof, L.J., Fingerprinting microbial assemblages from the oxic/anoxic chemocline of the Black Sea, Appl. Environ. Microbiol., 2003, vol. 69, no. 11, pp. 6481–6488.
Neretin, L.N., Abed, R.M., Schippers, A., Schubert, C.J., Kohls, K., and Kuypers, M.M., Inorganic carbon fixation by sulfate-reducing bacteria in the Black Sea water column, Environ. Microbiol., 2007, vol. 9, no. 12, pp. 3019–3024.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A.L. Bryukhanov, V.A. Korneeva, N.V. Pimenov, 2015, published in Vestnik Moskovskogo Universiteta. Biologiya, 2015, No. 4, pp. 36–40.
About this article
Cite this article
Bryukhanov, A.L., Korneeva, V.A. & Pimenov, N.V. Detection of anaerobic sulfate-reducing bacteria in oxygen-containing upper water layers of the Black and Baltic Seas. Moscow Univ. Biol.Sci. Bull. 70, 184–188 (2015). https://doi.org/10.3103/S0096392515040057
Received:
Published:
Issue Date:
DOI: https://doi.org/10.3103/S0096392515040057