Abstract
A sensitive and disposable electrochemical impedance biosensor to detect Japanese encephalitis virus (JEV) was developed based on a gold nanoparticle (AuNP)-modified screen-printed carbon electrode (SPCE). A biosensor was fabricated through covalent grafting of a mixed self-assembled monolayer on AuNPs with a specific antibody. To detect JEV and achieve signal amplification, the horseradish peroxidase (HRP)-labeled second antibody was linked to the biosensor through a sandwich immunity reaction. HRP was used to catalyze 4-chloro-1-naphthol oxidation to produce an insoluble precipitate, which introduced a barrier to electron transfer on the electrode. Electrochemical impedance spectroscopy (EIS) was used to monitor the precipitation on the electrode. The electron-transfer resistance (Ret) of the biosensor was directly correlated with the concentration of JEV in the solution. Under optimal conditions, the method generated a linear response range between 500 and 5 × 105 pfu mL−1, and the detection limit was 167 pfu mL−1. The biosensor exhibited good selectivity against other viruses.
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T. P. Endy and A. Nisalak, Curr. Top. Microbiol., 2002, 267, 11.
G. Watt and K. Jongsakul, Am. J. Trop. Med. Hyg., 2003, 68, 704.
Y. Li, L. Hou, J. Ye, X. Liu, H. Dan, M. Jin, H. Chen, and S. Cao, J. Virol. Methods, 2010, 768, 51.
S. H. Huang, T. C. Yang, M. H. Tsai, I. S. Tsai, H. C. Lu, P. H. Chuang, L. Wan, Y. J. Lin, C. H. Lai, and C. W. Lin, Nanotechnology, 2008, 19, 4050101.
J. Hirota, H. Nishi, H. Matsuda, H. Tsunemitsu, and S. Shimiz, J. Vet. Med. Sci., 2010, 72, 369.
B. R. Gulati, H. Singha, B. K. Singh, N. Virmani, S. K. Khurana, and R. K. Singh, J. Vet. Sci., 2011, 12, 341.
S. C. Barros, F. Ramos, L. Z. Zéb, M. J. Alvesb, T. Fagulhaa, M. Duartea, Ma. Henriquesa, T. Luîsa, and M. Fevereiroa, J. Virol. Methods, 2013, 193, 554.
M. M. Parida, S. R. Santhosh, P. K. Dash, N. K. Tripathi, P. Saxena, S. Ambuj, A. K. Sahni, P. V. Lakshmana Rao, and K. Morita, J. Clin. Microbiol., 2006, 44, 4172.
L. Hou, Z. Q. Gao, M. D. Xu, X. Cao, X. P. Wu, G. N. Chen, and D. P. Tang, Biosens. Bioelectron., 2014, 54, 365.
J. P. Metters, R. O. Kadara, and C. E. Banks, Analyst, 2011, 136, 1067.
T. Q. Huy, N. T. H. Hanh, N. T. Thuy, P. V. Chung, P. T. Nga, and M. A. Tuan, Talanta, 2011, 86, 271.
L. Ianeselli, G. Grenci, C. Callegaric, M. Tormenb, and L. Casalis, Biosens. Bioelectron., 2014, 55, 1.
F. Li, L. Mei, Y. M. Li, K. H. Zhao, H. C. Chen, P. Wu, Y. G. Hu, and S. B. Cao, Biosens. Bioelectron., 2011, 26, 4253.
L. Hou, Y. Tang, M. D. Xu, Z. Q. Gao, and D. P. Tang, Anal. Chem., 2014, 86, 8352.
R. Akter, M. A. Rahman, and C. K. Rhee, Anal. Chem., 2012, 84, 6407.
L. Hou, X. P. Wu, G. N. Chen, H. H. Yang, M. H. Lu, and D. P. Tang, Biosens. Bioelectron., 2015, 68, 487.
F. Yang, J. Han, Y. Zhuo, Z. H. Yang, Y. Q. Chai, and R. Yuan, Biosens. Bioelectron., 2014, 55, 360.
M. Tudorache and C. Bala, Anal. Bioanal. Chem., 2007, 388, 565.
G. Marrazza, I. Chianella, and M. Mascini, Anal. Chim. Acta, 1999, 387, 297.
S. Jampasa, W. Wonsawat, N. Rodthongkum, W. Siangproh, P. Yanatatsaneejit, T. Vilaivan, and O. Chailapakul, Biosens. Bioelectron., 2014, 54, 428.
Y. H. Zhang, X. H. Geng, J. J. Ai, Q. Gao, H. L. Qi, and C. X. Zhang, Sens. Actuators, B, 2015, 221, 1535.
J. Liu, X. Q. Yuan, Q. Gao, H. L. Qi, and C. X. Zhang, Sens. Actuators, B, 2012, 162, 384.
Q. Gao, Y. Guo, W. Y. Zhang, H. L. Qi, and C. X. Zhang, Sens. Actuators, B, 2011, 153, 219.
J. Liu, X. Q. Yuan, Q. Gao, H. L. Qi, and C. X. Zhang, Sens. Actuators, B, 2012, 162, 384.
R. Jurczakowski and P. Polczynski, J. Phys. Chem. C, 2014, 118, 7980.
B. Rezaei, N. Majidi, H. Rahmani, and T. Khayamian, Biosens. Bioelectron., 2011, 26, 2130.
H. Liu, Z. J. Liu, J. Jing, J. Q. Ren, Y. Y. Liu, H. H. Guo, M. Fan, H. J. Lu, and N. Y. Jin, Vector. Borne. Zoonotic. Dis., 2012, 12, 1042.
J. Deng, J. J. Pei, H. C. Gou, Z. D. Ye, and C. C. Liu, and J. D. Chen, J. Virol. Methods, 2015, 213, 98.
Acknowledgments
The authors gratefully acknowledge the financial support from the National Nature Science Foundation of China (No. 21175089), Program for Innovative Research Team in Shaanxi Province (No. 2014KCT-28), and the Natural Science Basic Research Plan in Shaanxi Province of China (Nos. 2013SZS08-Z01 and 2013SZS08-P01).
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Geng, X., Zhang, F., Gao, Q. et al. Sensitive Impedimetric Immunoassay of Japanese Encephalitis Virus Based on Enzyme Biocatalyzed Precipitation on a Gold Nanoparticle-modified Screen-printed Carbon Electrode. ANAL. SCI. 32, 1105–1109 (2016). https://doi.org/10.2116/analsci.32.1105
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DOI: https://doi.org/10.2116/analsci.32.1105