Abstract
A dual-electrochemical sensor based on a test-strip assay with immunochemistry and enzyme reactions has been developed for the determination of albumin and creatinine. Each nitrocellulose membrane with an immobilization area of an anti-albumin antibody or three enzymes was prepared in the device with three working electrodes for measuring albumin, creatinine, and ascorbic acid, as well as an Ag/AgCl electrode used as a counter/pseudo-reference electrode. The reactions of three enzymes were initiated by flowing a solution containing creatinine to detect an oxidation current of hydrogen peroxide. A sandwich-type immunocomplex was formed by albumin and antibody labeled with glucose oxidase (GOx). Captured GOx catalyzed the reduction of Fe(CN)63- to Fe(CN)64-, which was oxidized electrochemically to determine the captured albumin. The responses for creatinine and albumin increased with the concentrations in millimolar order and over the range 18.75–150 μg mL−1, respectively. The present sensor would be a distinct demonstration for producing quantitative dual-assays for various biomolecules used for clinical diagnoses.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
F. Tsagkogeorgas, M. Ochsenkuhn-Petropoulou, R. Niessner, D. Knopp, Anal. Chim. Acta, 2006, 573-574, 133.
R. F. Zuk, V. K. Ginsberg, T. Houts, J. Rabbie, H. Merrick, E. F. Ullman, M. M. Fischer, C. C. Sizto, S. N. Stiso, D. J. Litman, Clin. Chem., 1985, 31, 1144.
X. Mao, Y. Ma, A. Zhang, L. Zhang, L. Zeng, G. Liu, Anal. Chem., 2009, 81, 1660.
A. K. Ellerbee, S. T. Phillips, A. C. Siegel, K. A. Mirica, A. W. Martinez, P. Striehl, N. Jain, M. Prentiss, G. M. Whitesides, Anal. Chem., 2009, 81, 8447.
A. W. Martinez, S. T. Phillips, M. J. Butte, G. M. Whitesides, Angew. Chem., Int. Ed., 2007, 46, 1318.
A. W. Martinez, S. T. Phillips, G. M. Whitesides, E. Carrilho, Anal. Chem., 2010, 82, 3.
W. Dungchai, O. Chailapakul, C. S. Henry, Analyst, 2011, 136, 77.
Z. Nie, F. Deiss, X. Liu, O. Akbulut, G. M. Whitesides, Lab Chip, 2010, 10, 3163.
Z. Nie, C. A. Nijhuis, J. Gong, X. Chen, A. Kumachev, A. W. Martinez, M. Narovlyansky, G. M. Whitesides, Lab Chip, 2010, 10, 477.
W. Dungchai, O. Chailapakul, C. S. Henry, Anal. Chem., 2009, 81, 5821.
R. F. Carvalhal, M. Simaö Kfouri, M. H. de Oliveira Piazetta, A.L. Gobbi, L.T. Kubota, Anal. Chem., 2010, 82, 1162.
X. Mao, M. Baloda, A. S. Gurung, Y. Lin, G. Liu, Electrochem. Commun., 2008, 10, 1636.
H. Niana, J. Wang, H. Wu, J.-G. Lo, K.-H. Chiu, J. G. Pounds, Y. Lin, Anal. Chim. Acta, 2012, 713, 50.
Q. Yang, X. Gong, T. Song, J. Yang, S. Zhu, Y. Li, Y. Cui, Y. Li, B. Zhang, J. Chang, Biosens. Bioelectron., 2011, 30, 145.
Y.-Y. Lin, J. Wang, G. Liu, H. Wu, C. M. Wai, Y. Lin, Biosens. Bioelectron., 2008, 23, 1659.
G. Liu, Y.-Y. Lin, J. Wang, H. Wu, C. M. Wai, Y. Lin, Anal. Chem., 2007, 79, 7644.
D. Du, J. Wang, L. Wang, D. Lu, Y. Lin, Anal. Chem., 2012, 84, 1380.
Z.-X. Zou, J. Wang, H. Wang, Y.-Q. Li, Y. Lin, Talanta, 2012, 94, 58.
L. Wang, D. Lub, J. Wang, D. Du, Z. Zou, H. Wang, J. N. Smith, C. Timchalk, F. Liu, Y. Lin, Biosens. Bioelectron., 2011, 26, 2835.
K. Inoue, P. Ferrante, Y. Hirano, T. Yasukawa, H. Shiku, T. Matsue, Talanta, 2007, 73, 886.
K. S. Lee, T. Kim, M. Shin, W. Lee, J. Park, Anal. Chim. Acta, 1999, 380, 17.
Y. Kiba, Y. Otani, T. Yasukawa, F. Mizutani, Electrochim. Acta, 2012, 81, 14.
American Diabetes Association: Diabetic nephropathy, Diabetes Care, 2003, 26, S94.
M. Parsons, D. J. Newman, M. Pugia, R. G. Newall, C. P. Price, Clin. Nephrol., 1999, 51, 220.
R. E. Gilbert, A. Akdeniz, G. Jerums, Diabetes Res. Clin. Pract., 1997, 35, 57.
O. Giampietro, G. Penno, A. Clerico, L. Cruschelli, A. Lucchetti, M. Nannipieri, M. Cecere, L. Rizzo, R. Navalesi, Acta Diabetol., 1992, 28, 239.
G. F. Watts, J. E. Bennett, D. J. Rowe, R. W. Morris, W. Gatling, K. M. Shaw, A. Polak, Clin. Chem., 1986, 32, 1544.
K. Yoshimoto, E. Kaneko, T. Yotsuyanagi, Chem. Lett., 2000, 29, 6.
J. Isoe and E. Kaneko, Chem. Lett., 2006, 35, 922.
E. Kaneko, H. Yasuda, A. Higurashi, H. Yoshimura, Analyst, 2010, 135, 1564.
C. P. Price, R. G. Newall, J. C. Boyd, Clin. Chem., 2005, 51, 1577.
M. Guy, J. K. Borzomato, R. G. Newall, P. A. Kalra, C. P. Price, Ann. Clin. Biochem., 2009, 46, 468.
D. J. Newman, M. J. Pugia, J. A. Lott, J. F. Wallace, A. M. Hiar, Clin. Chim. Acta, 2000, 294, 139.
S. S. Waikar, V. S. Sabbisetti, J. V. Bonventre, Kidney Int., 2010, 78, 486.
J. A. Weber and A. P. Van Zenten, Clin. Chem., 1991, 37, 695.
I. Kubo, I. Karube, S. Suzuki, Anal. Chim. Acta, 1983, 151, 371.
T. Tsuchida and K. Yoda, Clin. Chem., 1983, 29, 51.
E. J. Kim, T. Haruyama, Y. Yanagida, E. Kobatake, M. Aizawa, Anal. Chim. Acta, 1999, 394, 225.
Acknowledgments
This work was partly supported by JSPS KAKENHI Grant No. 26288071.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Rights and permissions
About this article
Cite this article
Yasukawa, T., Kiba, Y. & Mizutani, F. A Dual Electrochemical Sensor Based on a Test-strip Assay for the Quantitative Determination of Albumin and Creatinine. ANAL. SCI. 31, 583–589 (2015). https://doi.org/10.2116/analsci.31.583
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.2116/analsci.31.583