Abstract
An electrochemical sensor for phosphate detection in body fluids was developed based on the hydration transition of magnesium hydrogen phosphate (newberyite, MgHPO4·3H2O). The sensor was fabricated through incubation of a multiwalled carbon nanotube/Nafion (MWCNT/Nafion) modified glassy carbon electrode (GCE) in magnesium phosphate solution, where MgHPO4·3H2O was self-assembled on the electrode surface (denoted as MgP/MWCNT/Nafion). An electrooxidation peak at 1.0 V vs. Ag/AgCl was observed when the as-prepared electrode was subjected to a differential pulse voltammetry (DPV) scan in the presence of phosphate in acetate solution. When the DPV scan was performed in 0.4–1.3 V vs. Ag/AgCl, a linear relationship was observed between the peak height and the phosphate concentration in the range from 0.01 to 25 μM in the presence of 0.1 mM Mg2+ in the acetate solution with a limit of detection of 32 nM. And the sensor was successfully applied for phosphate detection in human urine and saliva samples with recoveries of 94.7–104.4 and 96–103.3%, respectively.
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Acknowledgements
This work was supported by the Natural National Science Foundation of China (51973083), National First-Class Discipline Program of Food Science and Technology (JUFSTR20180301), China Postdoctoral Science Foundation (2019M651688), Fundamental Research Funds for the Central Universities (JUSRP22027), and MOE & SAFEA for the 111 Project (B13025). Q. S. would like to acknowledge the work of the Central Laboratory, School of Chemical and Material Engineering, Jiangnan University.
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Chen, Q., Sun, S., Ran, G. et al. Electrochemical Detection of Phosphate Ion in Body Fluids with a Magnesium Phosphate Modified Electrode. ANAL. SCI. 37, 1247–1252 (2021). https://doi.org/10.2116/analsci.20P415
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DOI: https://doi.org/10.2116/analsci.20P415