Abstract
This research work presents a simple, sensitive, selective, economic, and widely applicable and interferences-free spectrofluorimetric method estimating moxifloxacin in the pure form, commercial formulations and biological samples. The method is based on the reaction of moxifloxacin with Ce(IV) in an acidic medium to generate fluorescent active species Ce(III). The excitation and emission of the fluorescent species are 250 and 362 nm, respectively. Different variables that might influence the oxidation of moxifloxacin, including the Ce(IV) concentration and volume, the effect of temperature and the heating time, the type of acids and its concentration were analyzed and boosted. The linearity was observed in the concentration range of 0.2 - 5.0 (μg mL−1 with a correlation coefficient of 0.9991. The limit of detection and the limit of quantification were calculated and observed to be 0.016 and 0.056 μg mL−1 respectively. The effects of the common excipients and some co-administrated drugs usually used in the determination of moxifloxacin were investigated, and no interferences were noted. The planned method has been successfully practical for the analysis of moxifloxacin in its pure form, in pharmaceutical products and in biological samples. The obtained percent recoveries ranged from 95.50 to 101.37% for pharmaceutical products and from 95.15 to 103.18% for human blood plasma and urine.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
F. Varanda, M. J. P. D. Melo, A. I. Caco, F. Dohrn, A. F. Makrydaki, E. Voutsas, D. Tassios, and M. I. Marrucho, Ind. Eng. Chem. Res., 2006, 45, 6368.
K. Kaur, A. Kumar, A. K. Malik, B. Singh, and A. L. J. Rao, Crit. Rev. Anal. Chem., 2008, 38, 2.
A. P. Dewani, B. B. Barik, S. K. Kanungo, B. R. Wattyani, and A.V. Chandewar, J. Sci. Res., 2011, 6, 192.
E. R. Mondal, K. Das, and P. Mukherjee, Asian Pac. J. Cancer Prev., 2004, 5, 196.
A. Mahesh, B. E. Al-Dhubaib, I. A. Alhaider, and A. B. Nair, Chem. Cent. J., 2012, 6, 105.
C. M. Culley, M. K. Lacy, N. Klutman, and B. E. Edwards, Am. J. Health Syst. Pharm., 2001, 58, 379.
K. Vishwanathan, G. M. Bartlett, and T. J. Stewart, J. Pharm. Biomed. Anal., 2002, 30, 961.
G. A. M. Trindad, M. G. da Silva, and S. V. Ferreira, Microchem. J., 2005, 81, 209.
S. K. Motwani, S. Chopra, J. F. Ahmad, and K. R. Khar, Spectrochim. Acta, Part A, 2007, 68, 250.
A. Julia, B. Balfour, and L. R. Wieseman, Drugs, 1999, 57, 363.
S. K. Motwani, R. K. Khar, F. J. Ahmad, S. Chopra, K. Kohli, and S. Talegaonkar, Anal. Chim. Acta., 2007, 582, 75.
P. Djurdjevic, A. Ciric, A. Djurdjevic, and M. J. Stankov, J. Pharm. Biomed. Anal., 2009, 50, 117.
N. Srinivas, L. Narasu, B. P. Shankar, and R. Mullangi, Biomed. Chromatogr., 2008, 22, 1288.
M. A. G. Trindade, G. M. da Silva, and V. S. Ferreira, Microchem. J., 2005, 81, 209.
M. H. Langlois, M. Montagut, J. P. Dubost, J. Grellet, and M. C. Saux, J. Pharm. Biomed. Anal., 2005, 37, 389.
S. K. Motwani, S. Chopra, F. J. Ahmad, and R. K. Khar, Spectrochim. Acta, Part A, 2007, 68, 250.
K. Veshwanathan, M. G. Bartlett, and J. T. Stewart, J. Pharm. Biomed. Anal., 2002, 30, 961.
M. N. Saraf, G. J. G. Khan, C. Tridevi, and K. Soni, Indian Drugs, 2005, 42, 375.
K. Kalyani, P. Sowjanya, T. Rajesh, and G. V. Kumar, Int. J. Chem. Pharm. Sci., 2015, 3, 2073.
J. G. Moller, H. Stass, R. Heinig, and G. Blaschke, J. Chromatogr. B, 1998, 716, 325.
N. Pradhan, H. Rajkhowa, H. Giri, and B. Shrestha, Int. J. Pharm. Sci., 2015, 7, 21.
R. Kant, R. Bodla, R. Bhutani, and G. Kapoor, Int. J. Pharm. Sci., 2015, 7, 316.
J. A. Ocana, F. J. Brragain, and M. Callejon, Analyst, 2000, 125, 2322.
U. H. Shah, Int. J. Pharm. Sci, 2013, 5, 252.
D. Predrag, C. Andrija, D. Aleksandra, and J. S. Milena, J. Pharm. Biomed. Anal., 2009, 50, 117.
N. T. Kailash, S. A. Swati, G. S. Neha, and R. S. Seema, Der Pharma Chemica, 2012, 4, 1180.
A. Laban-Djurdjevic, M. Jelikic-Stankov, and P. Djurdjevic, J. Chromatogr. B, 2006, 844, 104.
Acknowledgments
The authors are grateful to the Bacha Khan University Charsadda KPK, Pakistan for providing the facility to conduct this reach work.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Khan, M.N., Ali, W., Shah, Z. et al. A Validated Spectrofluorimetric Method for the Determination of Moxifloxacin in Its Pure Form, Pharmaceutical Preparations, and Biological Samples. ANAL. SCI. 36, 361–366 (2020). https://doi.org/10.2116/analsci.19P370
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.2116/analsci.19P370