Abstract
In this study, we developed an electrochemical sensor for ochratoxin A (OTA) by using an aptamer having a dithiol-based anchor, which exhibited higher stability on a gold electrode than a monothiol-based aptamer because of its two anchors. The sensor was also based on a signal-on scheme that produces a signal current resulting from structure-switching of the aptamer upon interaction with OTA. For simple fabrication of this sensor, the non-covalent interaction of methylene blue with the aptamer was also employed as an electrochemical indicator. In this study, the performance of the sensor, including the dissociation constant of the aptamer-OTA complex, was characterized. The proposed sensor exhibited high reproducibility and enough sensitivity to detect the minimum amount of OTA required for the analysis of real food samples with a limit of detection of 113 pM.
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Acknowledgments
A part of this study was supported by the Hokkaido University microstructural characterization platform as a program of “Nanotechnology Platform” of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. D. N. M. thanks the international educational program “Advanced Graduate School of Chemistry and Materials Science (AGS)” of the Graduate School of Chemical Sciences and Engineering, Hokkaido University.
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Mazaafrianto, D.N., Ishida, A., Maeki, M. et al. An Electrochemical Sensor Based on Structure Switching of Dithiol-modified Aptamer for Simple Detection of Ochratoxin A. ANAL. SCI. 35, 1221–1226 (2019). https://doi.org/10.2116/analsci.19P240
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DOI: https://doi.org/10.2116/analsci.19P240