Abstract
We rationally designed an ultrasensitive and label-free sensing platform for determination of cadmium (Cd). The sensing platform contains G-quadruplex-Cd(II) specific aptamer (GCDSA) constructed by incorporating G-rich sequence at the end of 5' and the critical domain of the Cd-4 aptamer. GCDSA designed act as both a special recognition sequence for Cd2+ and a signal DNAzyme. In absence of Cd2+, GCDSA may mainly exist in a random coil sequence. Upon addition of Cd2+, GCDSA could probably be induced to fold into a G-quadruplex structure. The generation of plentiful active G-quadruplex interacts with hemin to form a peroxidase-like DNAzyme, leading to increased absorbance signal of the sensing system. A4 was directly proportional to the two segments of concentrations for Cd2+, with the detection of limit of 0.15 nM. The proposed method avoids the labeled oligonucleotides and allows directly quantitative analysis of the samples by cheap instruments, with an excellent dynamic range.
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Acknowledgments
This work is subsidized by the National Natural Science Foundation of China (No. 81502850), the Natural Science Foundation of Hunan Province in China (No. 2015JJ2122) and Undergraduate inquiry learning and innovative experimental project in Hunan province (No. 336).
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Zhou, B., Chen, YT., Yang, XY. et al. An Ultrasensitive Colorimetric Strategy for Detection of Cadmium Based on the Peroxidase-like Activity of G-Quadruplex-Cd(II) Specific Aptamer. ANAL. SCI. 35, 277–282 (2019). https://doi.org/10.2116/analsci.18P248
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DOI: https://doi.org/10.2116/analsci.18P248