Abstract
We developed an amperometric glucose biosensor based on glucose oxidase (GOx) embedded in zinc oxide (ZnO)- chitosan (CS) hybrid composite films on electrodeposited Pt-Fe(III). This sensor exhibited a fast amperometric response (less than 10 s) to glucose, linearity from 10 μM to 11.0 mM of glucose with a detection limit of 1.0 μM (S/N = 3) and sensitivity of 30.70 μA mM 1 cm−2. An apparent Michaelis-Menten constant of 5.19 mM indicated high affinity between glucose and GOx immobilized in the ZnO-CS films. The effect of interferences such as uric acid, ascorbic acid, and acetaminophen on the performance of this sensor was negligible. In addition, this sensor retained 87% of its initial performance after two weeks of storage at 4°C, indicating that the hybrid composite films allowed successful immobilization of GOx with its high enzymatic activity.
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Acknowledgments
This work was supported by the National Research Foundation of Korea (2016R1C1B2011912) for Y.-R. K.
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Paik, ES., Kim, YR. & Hong, HG. Amperometric Glucose Biosensor Utilizing Zinc Oxide-chitosan-glucose Oxidase Hybrid Composite Films on Electrodeposited Pt-Fe(III). ANAL. SCI. 34, 1271–1276 (2018). https://doi.org/10.2116/analsci.18P054
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DOI: https://doi.org/10.2116/analsci.18P054