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Proinflammatory cytokine responses in patients with psoriasis

  • Research Article
  • Published:
European Cytokine Network

Abstract

Background

Psoriasis is one of the most common, immune-mediated, chronic inflammatory skin diseases. Proinflammatory cytokines play an important pathogenetic role at a local level.

Objective

To assess whether the proinflammatory cytokines IL-1β, IL-6, IL-17, IL-22 and TNF-α are released systemically during psoriasis.

Methods

Peripheral blood mononuclear cells (PBMCs) were isolated from 30 patients with psoriasis and 30 healthy volunteers. Cytokine production was assessed in supernatants using an enzyme immunoassay after stimulation of PBMCs with microbial stimuli. In addition, flow cytometry was used to determine the subsets of monocytes involved and the intracellular TNF-α production in monocytes.

Results

IL-17 levels were significantly higher in the supernatants of PBMCs from psoriatic patients after stimulation with phytohemagglutinin. TNF-α production was also significantly higher in cells from psoriatic patients after stimulation with all stimuli, as compared with health volunteers. Similar changes were not found for the other cytokines. A statistically significant difference was observed between patients and controls for inflammatory CD14+/CD16+ monocytes (p<0.0001) and patrolling CD14-/CD16+ monocytes.

Conclusion

Hyper-production of TNF-α is documented in psoriasis. These results support the concept that there is a systemic, proinflammatory component in psoriasis.

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Authors and Affiliations

  1. 2nd Department of Dermatology and Venereology, National and Kapodistrian University of Athens, Medical School, Dragoumi 5, 12141, Athens, Greece

    Anargyros Kouris, Alexandros Katoulis, Sofia Giatrakou, Evangelia Papadavid & Nikolaos Stavrianeas

  2. 4th Department of Internal Medicine, National and Kapodistrian University of Athens, Medical School, Athens, Greece

    Aikaterini Pistiki, Marianna Georgitsi & Evangelos J. Giamarellos-Bourboulis

  3. Department of Medicine and Nijmegen Institute for Infection, Inflammation and Immunity, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands

    Mihai G. Netea & Evangelos J. Giamarellos-Bourboulis

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  1. Anargyros Kouris
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  2. Aikaterini Pistiki
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  3. Alexandros Katoulis
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  4. Marianna Georgitsi
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  9. Evangelos J. Giamarellos-Bourboulis
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Correspondence to Anargyros Kouris.

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Kouris, A., Pistiki, A., Katoulis, A. et al. Proinflammatory cytokine responses in patients with psoriasis. Eur Cytokine Netw 25, 63–68 (2014). https://doi.org/10.1684/ecn.2014.0358

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  • DOI: https://doi.org/10.1684/ecn.2014.0358

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