Abstract
A rapid decline in egg production of laying hens begins after 480 d of age. Such a rapid decrease results predominantly from the ovarian aging, accompanied by endocrine changes, decreased yolk synthesis and accumulation, and the reduction in follicles selected into the preovulatory hierarchy. In this study, hens at 90, 150, 280, and 580 d old (D90, D150, D280, and D580, respectively) were compared for yolk precursor formation in the liver to elucidate effects of aging on laying performance. The results showed that liver lipid synthesis increased remarkably in hens from D90 to D150, but decreased sharply at D580 as indicated by the changes in triglyceride (TG) levels. This result was consistent with the age-related changes of the laying performance. The levels of liver antioxidants and total antioxidant capacity decreased significantly in D580 hens and the methane dicarboxylic aldehyde in D580 hens was much higher than that at other stages. The serum 17β-estradiol level increased from D90 to D280, but decreased at D580 ({itP}<0.05). The expression of estrogen receptor α and β mRNAs in the liver displayed similar changes to the serum 17β-estradiol in D580 hens. Expressions of the genes related to yolk precursor formation and enzymes responsible for fat acid synthesis were all decreased in D580 hens. These results indicated that decreased yolk precursor formation in the liver of the aged hens resulted from concomitant decreases of serum 17β-estradiol level, transcription levels of estrogen receptors and critical genes involved in yolk precursor synthesis, and liver antioxidant status.
中文概要
目的
比较不同日龄蛋鸡血清雌激素的水平、肝脏雌激 素受体基因和卵黄前体物生成相关基因表达量、 “肝脏-血液-卵巢轴”中甘油三酯和胆固醇的含 量和肝脏抗氧化能力的动态变化,揭示蛋鸡卵黄 前体物生成随着年龄增长的变化规律。
创新点
首次发现在蛋鸡衰老过程中雌激素作用衰退以及 肝脏抗氧化能力降低致使肝脏中卵黄前体物合 成下降,最终导致蛋鸡的产蛋性能下降。
方法
取90、150、280 和580 日龄蛋鸡各10 只,检测 血清中雌激素水平、肝脏雌激素受体基因表达水 平、肝脏中卵黄前体物质合成和脂质代谢相关基 因表达水平、“肝脏-血液-卵巢轴”中甘油三酯 和胆固醇的含量及肝脏抗氧化能力,揭示蛋鸡卵 黄前体物生成在衰老过程中的变化规律。
结论
在蛋鸡衰老过程中,血清雌激素水平、肝脏雌激 素受体基因和卵黄前体物质合成相关基因表达 量降低,肝脏抗氧化能力降低,引起肝脏合成甘 油三酯的能力下降,最终导致卵黄前体物质合成 能力降低。
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Acknowledgements
We sincerely thank Chang-quan GUO, Guang LIU, and Rong-xia LI (College of Animal Sciences, Zhejiang University, Hangzhou, China) for their help in the experiments.
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Project supported by the National Natural Science Foundation of China (Nos. 31472160 and 31272525)
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Liu, Xt., Lin, X., Mi, Yl. et al. Age-related changes of yolk precursor formation in the liver of laying hens. J. Zhejiang Univ. Sci. B 19, 390–399 (2018). https://doi.org/10.1631/jzus.B1700054
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DOI: https://doi.org/10.1631/jzus.B1700054