Abstract
In this study, prevalence of the protozoan parasites from the genus Giardia spp, with zoonotic potential and worldwide dissemination, was accessed in young dogs, which are reported as having higher prevalence rates. With that purpose, 49 animals from the Grupo de Intervenção Cinotécnico of the Guarda Nacional Republicana (Portuguese Gendarmerie Canine Unit) were chosen. They were housed individually in areas with a high number of kennels (up to 100), with ages ragging from newborns to 10 years old. Dogs were divided in four groups, according their age: under 6 months (n = 16), 6–12 months (n = 6), 12–18 months (n = 13) and 18–24 months (n = 14), comprising 22 females and 27 males. Fecal samples were collected from every animal and all were submitted to two different diagnostic tests, a passive flotation technique with a ZnSO4 solution and a detection of fecal antigen using a commercially available ELISA test (Witness® Giardia–Zoetis). From the 49 samples, 5 (10.2%) were considered positive with ZnSO4 flotation technique and 6 (12.24%) with the Witness® Giardia test. When considering the combination of both tests, 5 animals (10.2%) were considered positive. Of these, 3 (60%) were from the group under 6 months old, 1 (20%) from the 6–12 months and 1 from the 18–24 (20%) months. Within each group, in the under 6 months group 18.75% (n = 16) were considered positive, 16.67% in the 6–12 month group (n = 6), 0% in the 12–18 month group (n=13) and 7.14% in 18–24 month group (n = 14). None of the animals had clinical signs and no significant differences were found when comparing prevalence according to age, breed or gender. A combination of fecal flotation and antigen ELISA tests have good sensitivity and are easy to perform in practice and, therefore, could be a good choice to perform a diagnostic and small animal veterinarians should have this possible diagnostic in mind when in the present of clinical signs, particularly in young dogs.
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Bajer A., Bednarska M., Rodo A. 2011. Risk factors and control of intestinal parasite infections in sled dogs in Poland. Veterinary Parasitology 175, 343–350. DOI: 10.1016/j.vetpar.2010.10.029
Ballweber L., Xiao L., Bowman D., Kahn G., Cama V. 2010. Giardiasis in dogs and cats: Update on epidemiology and public health significance. Trends in Parasitology 26 (4), pp. 180–189. DOI: 10.1016/j.pt.2010.02.005
Bowman D., Lucio-Forster A. 2010. Cryptosporidiosis and giardiasis in dogs and cats: Veterinary and public health importance. Experimental Parasitology 124, 121–127. DOI: 10.1016/j.exppara.2009.01.003
Bugg R., Robertson I., Elliot A., Thompson R. 1999. Gastrointestinal Parasites of Urban Dogs in Perth, Western Australia. The Veterinary Journal 157, 295–301. DOI: 10.1186/1756-3305-5-91
Carlin E., Bowman D., Scarlett J. 2006. Prevalence of giardia in symptomatic dogs and cats in the United States. Continuing Education for Veterinarians 28 (11A), 1–12. DOI: 10.1016/j.vetpar.2010.06.015
Castro-Hermida J., García-Presedo I., Almeida A., González-Warleta M., Costa J., Mezo M. 2011. Cryptosporidium spp. and Giardia duodenalis in two areas of Galicia (NW Spain). Science of the Total Environment 409, 2451–2459. DOI:10.1016/j.scitotenv.2011.03.010
Claerebout E., Casaert S., Dalemans A., De Wilde N., Levecke B., Vercruysse J., Geurden T. (2009. Giardia and other parasites in different dog populations in Nothern Belgium. Veterinary Parasitology 161, 41–46. DOI: 10.1016/j.vetpar.2008.11.024
Díaz V., Campos M., Lozano J., Manas I., Gonzalez J. 1996. Aspects of animal giardiosis in Granada province (southern Spain). Veterinary Parasitology 64, 171–176. DOI: 10.1016/0304- 4017(95)00923-X
Dryden M., Payne P., Smith V. 2006. Accurate Diagnosis of Giardia spp and Proper Fecal Examination Procedures. Veterinary Therapeutics, Volume 7, Number 1, 4–14. DOI: 10.1016/j.vetpar.2010.06.015
Dubná S., Langrová I., Nápravnik J., Jankovska I., Vadlecjh J., Pekár S., Fechtner J. 2007. The prevalence of intestinal parasites in dogs from Prague, rural areas, and shelters of the Czech Republic. Veterinary Parasitology 145, 120–128. DOI: 10.1016/j.vetpar.2006.11.006
Epe C., Rehkter G., Scnieder T., Lorentzen L., Kreienbrock L. 2010. Giardia in symptomatic dogs and cats in Europe - Results of a European study. Veterinary Parasitology 173, 32–38. DOI: 10.1016/j.vetpar.2010.06.015
Fiechter R., Deplazes P., Schnyder M. 2012. Control of Giardia infections with ronidazole and intensive hygiene management in a dog kennel. Veterinary Parasitology 187, 93–98. DOI: 10.1016/j.vetpar.2011.12.023
Fontanarrosa M., Vezzani D., Basabe J., Eiras D. 2006. An epidemiological study of gastrointestinal parasites of dogs from Southern Greater Buenos Aires (Argentina): Age, gender, breed, mixed infections, and seasonal and spatial patterns. Veterinary Parasitology 136, 283–295. DOI: 10.1016/j.vetpar.2010.01.018.
Geurden T., Berkvens D., Casaert S., Vercruysse J., Claerebout E. 2008. A Bayesian evaluation of three diagnostic assays for the detection of Giardia duodenalis in symptomatic and asymptomatic dogs. Veterinary Parasitology 157, 14–20. DOI: 10.1016/j.vetpar.2008.07.002
Hamnes I., Gjerde B., Robertson L. 2007. A longitudinal study on the occurrence of Cryptosporidium and Giardia in dogs during their first year of life. Acta Veterinaria Scandinavica, 49:22, 1–10. DOI: 10.1186/1751-0147-49-22
Itoh N., Muraoka N., Saeki H., Aoki M., Itagaki T. 2005. Prevalence of Giardia intestinalis infection in dogs of breeding kennels in Japan. The Journal of Veterinary Medical Science 67(7), 717–718. DOI: 10.1016/j.vetpar.2010.10.048
Katagiri S., Sequeira-Oliveira T. 2008. Prevalence of dog intestinal parasites and risk perception of zoonotic infection by dog owners in São Paulo State, Brazil. Zoonoses and Public Health 55(8–10): 406–13. DOI: 10.1111/j.1863-2378.2008.01163.x
Liu J., Lee S., Song K. 2007. Prevalence of canine giardiosis in South Korea. Research in Veterinary Science 84(3), 416–418. DOI: 10.1016/j.vetpar.2014.12.011
Martínez-Moreno F., Hernández S., López-Cobos E., Becerra C., Acosta I., Martínez-Moreno A. 2007. Estimation of canine intestinal parasites in Córdoba (Spain) and their risk to public health. Veterinary Parasitology 143, 7–13. DOI: 10.1016/j.vetpar.2006.08.004
Mircean V., Gyorke A., Cozma V. 2012. Prevalence and risk factors of Giardia duodenalis in dogs from Romania. Veterinary Parasitology 184, 325–329. DOI: 10.1016/j.vetpar.2011.08.022
Overgaauw P., Zutphen L., Hoek D., Yaya F., Roelfsema J., Pinelli E., Knapen F., Kortbeek L. 2009. Zoonotic parasites in fecal samples and fur from dogs and cats in the Netherlands. Veterinary Parasitology 163, 115–122. DOI: 10.1016/j.vetpar.2009.03.044
Palmer C., Thompson R., Traub R., Rees R., Robertson I. 2008. National study of the gastrointestinal parasites of dogs and cats in Australia. Veterinary Parasitology 151, 181–190. DOI: 10.1016/j.vetpar.2007.10.015
Papini R., Gorini G., Spaziani A., Cardini G. 2005. Survey on giardiosis in shelter dog populations. Veterinary Parasitology 128, 333–339. DOI: 10.1016/j.vetpar.2004.12.005
Rimhanen-Finne R., Enemark H., Kolehmainen J., Toropainen P., Hanninen M. 2007. Evaluation of immunofluorescence microscopy and enzyme-linked immunorbent assay in detection of Cryptosporidium and Giardia infections in asymptomatic dogs. Veterinary Parasitology 145, 345–348. DOI: 10.1016/ j.vetpar.2007.01.008
Rishniw M., Liotta J., Bellosa M., Bowman D., Simpson K. 2010. Comparison of 4 Giardia Diagnostic Tests in Diagnosis of Naturally Acquired Canine Chronic Subclincal Giardiasis. Journal of Veterinary Internal Medicine 24, 293–297. DOI: 10.1111/j.1939-1676.2010.0475.x
Rooney N., Gaines S., Hiby E. 2009. A practitioner’s guide to working dog welfare. Journal of Veterinary Behaviour: Clinical Applications and Research 4, 127–134. DOI: 10.1016/ j.jveb.2008.10.037
Scaramozzino P., Cave D., Berrilli F., D’Orazi C., Spaziani A., Mazzanti S., Scholl F., Liberato C. 2009. A study of the prevalence and genotypes of Giardia duodenalis infecting kenneled dogs. The Veterinary Journal 182, 231–234. DOI: 10.1016/j.tvjl.2008.07.003
Solarczyk P., Majewska A. 2010. A survey of the prevalence and genotypes of Giardia duodenalis infecting household and sheltered dogs. Parasitology Research 106, 1015–1019. DOI: 10.1007/s00436-010-1766-5
Szabová E., Juris P., Miterpáková M., Antolová D., Papajová I., Sefciková H. 2007. Prevalence of important zoonotic parasites in dog populations from the Slovak Republic. Helminthologia 44, 4: 170–176. DOI: 10.2478/s11687-007-0027-3
Tangtrongsup S. and Scorza V. 2010. Update on the Diagnosis and Management of Giardia spp Infections in Dogs and Cats. Topics in Companion Animal Medicine, Volume 25, Number 3, 155–162. DOI: 10.1053/j.tcam.2010.07.003
Thompson R. 2000. Giardiasis as re-emerging infectious disease and its zoonotic potencial. International Journal for Parasitology 30, 1259–1267. DOI: 10.1016/S0020-7519(00)00127-2
Thompson A. 2004. Epidemiology and zoonotic potential of Giardia infections. In C. Sterling, & A. Rodney, The pathogenic enteric protozoa: Giardia, Entamoeba, Cryptosporidium and Cyclospora. Tucson, Arizona: Kluwer Academic Publishers
Wolfe M. 1992. Giardiasis. Clinical Microbiology Reviews, Volume 5, Number 1, 93–100, pp. 1–13
World Health Organization. 1995. An Overview of selected curable sexually transmitted diseases. In: Global program on AIDS, World Health Organization, Geneva, Switzerland (unpublished)
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Alves, J., Santos, A. Prevalence of Giardia spp. in young dogs using a combination of two diagnostic methods. Acta Parasit. 61, 261–266 (2016). https://doi.org/10.1515/ap-2016-0036
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DOI: https://doi.org/10.1515/ap-2016-0036