Abstract
Few molecular studies have identified the current status of cystic echinococcosis in Egypt. The present study aimed to ascertain the genotype(s) of Echinococcus granulosus responsible for human hydatidosis in different Egyptian governorates (regions). Animal isolates were collected from 40 camels, 5 pigs and 44 sheep. 27 human isolates were included in the present study. Specific PCR was performed and followed by DNA sequencing for mitochondrial 12S ribosomal RNA gene and BLAST analysis. The sheep cysts were not hydatid cysts. G6 genotype (camel starin) predominates in human, camel and pig isolates. G7 genotype (pig strain) was detected in two human isolates and one pig isolate. G1 genotype (sheep strain) was detected in one human isolate from Yemen and in no animal isolates. This is the first record of G7 in Egypt and G1 in Yemen.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Aaty H.E., Abdel-Hameed D.M., Alam-Eldin Y.H., El-Shennawy S.F., Aminou H.A., Makled S.S., Darweesh S.K. 2012. Molecular genotyping of Echinococcus granulosus in animal and human isolates from Egypt. Acta Tropica, 121, 125–8. DOI: 10.1016/j.actatropica.2011.10.014
Abd El Baki M.H., El Missiry A.M., Abd El Aaty H.E., Mohamad A.A., Aminou H.A. 2009. Detection of G1 genotype of human cystic echinococcosis in Egypt. Journal of Egyptian Society of Parasitology, 39, 711–21
Aboelhadid S.M., El-Dakhly K.M., Yanai T., Fukushi H., Hassanin K.M. 2013. Molecular characterization of Echinococcus gran-ulosus in Egyptian donkeys. Veterinary Parasitology, 193, 292–296. DOI: 10.1016/j.vetpar.2012.11.019
Adwan G., Adwan, K., Bdir S., Abuseir S. 2013. Molecular characterization of Echinococcus granulosus isolated from sheep in Palestine. Experimental Parasitology, 134, 195–199. DOI: 10.1016/j.exppara.2013.03.024
Ahmed M.E., Eltom K.H., Musa N.O., Ali, I.A., Elamin F.M., Grobusch M.P., Aradaib I.E. 2013. First report on circulation of Echinococcus ortleppi in the one humped camel (Camelus dromedaries), Sudan. BMC Veterinary Research, 9, 127. DOI: 10.1186/1746-6148-9-127
Alvarez Rojas C.A., Romig T., Lightowlers M.W. 2014. Echinococ-cus granulosus sensu lato genotypes infecting humans - review of current knowledge. International Journal of Parasitology, 44, 9–18. DOI: 10.1016/j.ijpara.2013.08.008
Azab M.E., Bishara S.A., Helmy H., Oteifa N.M., El-Hoseiny L.M., Ramzy R.M., Ahmed M.A. 2004. Molecular characterization of Egyptian human and animal Echinococcus granulosus isolates by RAPD-PCR technique. Journal of Egyptian Society of Parasitology, 34, 83–96
Bardonnet K., Piarroux R., Dia L., Schneegans F., Beurdeley A., Godot, V., Vuitton D.A. 2002. Combined eco-epidemiological and molecular biology approaches to assess Echinococcus granulosus transmission to humans in Mauritania: occurrence of the ‘camel’ strain and human cystic echinococcosis. Transactions of the Royal Society of Tropical Medicine and Hygiene, 96, 383–386. DOI: 10.1016/S0035-9203(02)90369-X
Cardona G.A., Carmena D. 2013. A review of the global prevalence, molecular epidemiology and economics of cystic echinococ-cosis in production animals. Veterinary Parasitology, 192, 10–32. DOI: 10.1016/j.vetpar.2012.09.027
Dinkel A., Njoroge, E.M., Zimmermann A., Wälz M., Zeyhle E., Elmahdi I.E., Mackenstedt U., Romig T. 2004. A PCR system for detection of species and genotypes of the Echinococcus granulosus-complex, with reference to the epidemiological situation in eastern Africa. International Journal of Para-sitology, 34, 645–53. DOI: 10.1016/j.ijpara.2003.12.013
Farjallah S., Busi M., Mahjoub M.O., Slimane B.B., Said K., D’Amelio S. 2007. Molecular characterization of Echinococcus granulosus in Tunisia and Mauritania by mitochondrial rrnS gene sequencing. Parassitologia, 49, 239–246
Jabbar A., Narankhajid M., Nolan M.J., Jex A.R., Campbell B.E., Gasser R.B. 2011. A first insight into the genotypes of Echinococcus granulosus from humans in Mongolia. Molecular and Cellular Probes. 25, 49–54. DOI: 10.1016/j.mcp. 2010.11.001
Maillard S., Benchikh-Elfegoun M.C., Knapp J., Bart J.M., Koskei P., Gottstein B., Piarroux R. 2007. Taxonomic position and geographical distribution of the common sheep G1 and camel G6 strains of Echinococcus granulosus in three African countries. Parasitology Research, 100, 495–503. DOI: 10.1007/s00436-006-0286-9
McManus D.P. 2002. The molecular epidemiology of Echinococcus granulosus and cystic hydatid disease. Transactions of the Royal Society of Tropical Medicine and Hygiene, 96, 151–157. DOI: 10.1016/S0035-9203(02)90068-4
McManus D.P., Thompson R.C. 2003. Molecular epidemiology of cystic echinococcosis. Parasitology, 12, 37–51. DOI: 10.1017/S0031182003003524
Omer R.A., Dinkel A., Romig T., Mackenstedt U., Elnahas A.A., Aradaib I.E., Ahmed M.E., Elmalik K.H., Adam A. 2010. A molecular survey of cystic echinococcosis in Sudan. Veterinary Parasitology, 169, 340–346. DOI: 10.1016/j.vetpar. 2010.01.004
Pawlowski Z.S., Stefaniak J. 2003. Cystic echinococcosis (CE) in the Poznan region in the years 1990–2000. Przeglad Epidemio-logiczny, 57, 579–586
Sadjjadi M.S. 2006. Present situation of echinococcosis in the Middle East and Arabic North Africa. Parasitology International, 55, 197–202. DOI: 10.1016/j.parint.2005.11.030
Schneider R., Gollackner B., Schindl M., Tucek G., Auer H. 2010. Echinococcus canadensis G7 (pig strain): an underestimated cause of cystic echinococcosis in Austria. The American Journal of Tropical Medicine and Hygiene, 82, 871–874. DOI: 10.4269/ajtmh.2010.09-0639
Snabel V., Altintas N., D’Amelio S., Nakao M., Romig T., Yolasigmaz A., Gunes K., Turk M., Busi M., Huttner M., Sevcova D., Ito A., Altintas N., Dubinsky P. 2009. Cystic echinococ-cosis in Turkey: genetic variability and first record of the pig strain (G7) in the country. Parasitology Research, 105, 145–154.DOI: 10.1007/s00436-009-1376-2
Taha H.A. 2012. Genetic variations among Echinococcus granulosus isolates in Egypt using RAPD-PCR. Parasitology Research, 111, 1993–2000. DOI: 10.1007/s00436-012-3046-z
Tawfeek G.M., Elwakil H.S., Awad N.S., El-Hoseiny L., Thabet H.S., Sarhan R.M., Darweesh S.K., Anwar W.A. 2009. Genetic variability of antigen B among Echinococcus granulo-sus Egyptian isolates. Korean Journal of Parasitology, 47, 259–264. DOI: 10.3347/kjp.2009.47.3.259
Thompson R.C., McManus D.P. 2002. Towards a taxonomic revision of the genus Echinococcus. Trends in Parasiotology, 18, 452–457. DOI: 10.1016/S1471-4922(02)02358-9
Yanagida T., Mohammadzadeh T., Kamhawi S., Nakao M., Sadjjadi S.M., Hijjawi N., Abdel-Hafez S.K., Sako Y., Okamoto, Ito A. 2012. Genetic polymorphisms of Echinococcus granulosus sensu stricto in the Middle East. Parasitology International, 61, 599–603. DOI: 10.1016/j.parint.2012.05.014
Zhang L.H., Eslami A., Hosseini S.H., McManus D.P. 1998. Indication of the presence of two distinct strains of Echinococcus granulosus in Iran by mitochondrial DNA markers. The American Journal of Tropical Medicine and Hygiene, 59, 171–174
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Alam-Eldin, Y.H., Aaty, H.E.A. & Ahmed, M.A. Molecular characterization of cystic echinococcosis: First record of G7 in Egypt and G1 in Yemen. Acta Parasit. 60, 662–665 (2015). https://doi.org/10.1515/ap-2015-0094
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1515/ap-2015-0094