Abstract.
Using a microfluidic trap, we study the behavior of individual phospholipid vesicles in contact with fatty acids. We show that spontaneous fatty acids insertion inside the bilayer is controlled by the vesicle size, osmotic pressure difference across the membrane and fatty acids concentration in the external bath. Depending on these parameters, vesicles can grow spherically or become unstable and fragment into several daughter vesicles. We establish the phase diagram for vesicle growth and we derive a simple thermodynamic model that reproduces the time evolution of the vesicle volume. Finally, we show that stable growth can be achieved on an artificial cell expressing a simple set of bacterial cytoskeletal proteins, paving the way toward artificial cell reproduction.
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R.F. Gesteland, T.R. Cech, J.F. Atkins (Editors), The RNA World, 2nd edition (Cold Spring Harbor Press, Cold Spring Harbor, 1998)
L.E. Orgel, Crit. Rev. Biochem. Mol. Biol. 39, 99 (2004)
J.W. Szostak, D.P. Bartel, P.L. Luisi, Nature 409, 387 (2001)
I.A. Chen, P. Walde, Cold Spring Harb. Perspect. Biol. 2, a002170 (2010)
J.M. Gebicki, M. Hicks, Nature 243, 232 (1973)
W.R. Hargreaves, D.W. Deamer, Biochemistry 17, 3759 (1978)
M.M. Hanczyc, S.M. Fujikawa, J.W. Szostak, Science 302, 618 (2003)
P. Walde, R. Wick, M. Fresta, A. Mangone, P.L. Luisi, J. Am. Chem. Soc. 116, 11649 (1994)
T.F. Zhu, J.W. Szostak, J. Am. Chem. Soc. 131, 5705 (2009)
I.A. Chen, R.W. Roberts, J.W. Szostak, Science 305, 1474 (2004)
I.A. Chen, J.W. Szostak, Biophys J. 87, 988 (2004)
Markvoort et al., Biophys. J. 99, 1520 (2010)
P. Peterlin, V. Arrigler, K. Kogej, S. Svetina, P. Walde, Chem. Phys. Lipids 159, 67 (2009)
C. Hentrich, J.W. Szostak, Langmuir 30, 14916 (2014)
S. Pautot, B.J. Frisken, D.A. Weitz, Langmuir 19, 2870 (2003)
V. Noireaux, A. Libchaber, Proc. Natl. Acad. Sci. 101, 17669 (2004)
G.V. Richieri, R.T. Ogata, A.M. Kleinfeld, J. Biol. Chem. 267, 23495 (1992)
B.M. Davis, J. Richens, P. O’Shea, Biophys. J. 101, 245 (2011)
J. Shin, V.J. Noireaux, Biol. Eng. 4, 8 (2010)
Y.T. Maeda, T. Nakadai, J. Shin, K. Uryu, V. Noireaux, A. Libchaber, ACS Synth Biol. 1, 53 (2012)
J. Chalmeau, N. Monina, J. Shin, C. Vieu, V. Noireaux, Biochim. Biophys. Acta 1808, 271 (2011)
P.O. Olins, C.S. Devine, S.H. Rangwala, K.S. Kavka, Gene 73, 227 (1988)
A. Huebner et al., Lab Chip 9, 692 (2009)
R. Mercier, Y. Kawai, J. Errington, Cell 152, 997 (2013)
M. Osawa, D.E. Anderson, H.P. Erickson, Science 320, 792 (2008)
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Dervaux, J., Noireaux, V. & Libchaber, A.J. Growth and instability of a phospholipid vesicle in a bath of fatty acids. Eur. Phys. J. Plus 132, 284 (2017). https://doi.org/10.1140/epjp/i2017-11554-1
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DOI: https://doi.org/10.1140/epjp/i2017-11554-1