Abstract
Drosophila mercatorum is a species of neotropical origin. It is represented by two subspecies, marcatorum and pararepleta. Synanthropic subspecies D. mercatorum mercatorum spread into Eurasia in the mid-20th century, and to date, it is widespread over the territory of Europe and the former Soviet Union. The experiments were performed using the specimens of D. m. mercatorum from natural Asian populations, laboratory stock 2328 of D. mercatorum (displaying some morphological differences from Asian wild-type flies), the specimens of D. busckii and D. virilis, and the COI gene sequence of D. mercatorum from NCBI database, accession number, DQ471607. The specimens were investigated for the variability of the standard DNA sequences used for species identification (the cytochrome oxidase subunit I (COI) gene fragment and the ITS1–ITS2 region of rRNA genes). Sequences of the COI gene fragment from Asian D. m. mercatorum were identical between the specimens, but differed from the sequences of stock 2328, as well as from the NCBI sequence. Asian populations of D. mercatorum and stock 2328 were identical in the ITS1–ITS2 sequences. Low sequence divergence between the COI gene fragments, along with the absence of differences at the ITS1–ITS2 region of the rRNA genes indicate that the difference between the Asian populations of D. m. mercatorum and morphologically different stock 2328 are within the frames of intraspecies divergence.
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Original Russian Text © A.V. Ivannikov, O.V. Vaulin, Yu.A. Koromyslov, I.K. Zakharov, 2014, published in Vavilovskii Zhurnal Genetiki i Selektsii, 2014, Vol. 18, No. 2, pp. 308–314.
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Ivannikov, A.V., Vaulin, O.V., Koromyslov, Y.A. et al. Comparative molecular genetic study of the Asian population of Drosophila mercatorum (Diptera; Drosophilidae) at the COI gene fragment and the ITS1–ITS2 region of the rRNA genes. Russ J Genet Appl Res 4, 533–538 (2014). https://doi.org/10.1134/S2079059714060100
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DOI: https://doi.org/10.1134/S2079059714060100