Abstract
In the primary culture of bone marrow cells obtained from young (3-month-old) and old (20-month-old) rats, the change in the number of cells from day 0 to day 4, the pattern of cell morphotypes, and the lifespan of myelocytes, metamyelocytes, and stab and segment neutrophils was studied. It was shown that the number of bone marrow cells obtained from old animals (20-month-old) in the primary culture increased faster than the number of bone marrow cells in young animals (3-month-old). The presence of Copper-induced liver fibrosis in animals had a different effect on the rate of increase in the number of bone marrow cells obtained in young and old animals. The administration of 4 and 8 mmol of CuSO4 ⋅ 5H2O into the culture of bone marrow cells in young and old animals caused a dose-dependent inhibition of proliferative processes in the cells of young animals. When copper ions were administered into the culture of bone marrow cells obtained from old animals, the inhibition of proliferation was less pronounced than for young animals, and a concentration of 8 mmol of CuSO4 ⋅ 5H2O inhibited proliferation to a lesser extent than 4 mmol of CuSO4 ⋅ 5H2O. The presence of liver fibrosis in animals accelerated the process of bone marrow cell death in the primary culture in young and old animals. However, this effect was more pronounced in young animals. It is suggested that bone marrow cells undergo such epigenetic changes, which change their functional properties, during ontogeny.
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Bozhkov, A.I., Nikitchenko, Yu.V., Klimova, E.M., Linkevych, O.S., Lebid, K.M., Al-Bahadli, A.M.M., and Alsardia, M.M.A., Young and old rats have different strategies of metabolic adaptation to Copper induced liver fibrosis, Adv. Gerontol., 2017, vol. 7, no. 1, pp. 41–50.
Amend, S., Valkenburg, K., and Pienta, K., Murine hind limb long bone dissection and bone marrow isolation, J. Visualized Exp., 2016, vol. 2016, pp. 12–22.
Bara, J., Richards, R., Alini, M., et al., Concise review: Bone marrow-derived mesenchymal stem cells change phenotype following in vitro culture: implications for basic research and the clinic, Stem Cells, 2014, vol. 32, pp. 1713–1723.
Beane, O., Fonseca, V., Cooper, L., et al., Impact of aging on the regenerative properties of bone marrow-, muscle-, and adipose-derived mesenchymal stem/stromal cells, PLoS One, 2014, vol. 12, p. e115963.
Berardis, S., Sattwika, P., Najimi, M., et al., Use of mesenchymal stem cells to treat liver fibrosis: Current situation and future prospects, World J. Gastroenterol., 2015, vol. 21, pp. 742–758.
Bianco, P., Cao, X., Frenette, P., et al., The meaning, the sense and the significance: translating the science of mesenchymal stem cells into medicine, Nat. Med., 2013, vol. 19, pp. 35–42.
Blach-Olszewska, Z., Zaczynska, E., Gustaw-Rothenberg, K., et al., The innate immunity in Alzheimer disease— relevance to pathogenesis and therapy, Curr. Pharm. Des., 2015, vol. 21, pp. 3582–3588.
Bozhkov, A., Kabachnyy, V., Kolot, N., et al., Hormesis effect and the influence of ultra-low glycosides doses on the bone marrow cells proliferative activity in culture, J. Harmonized Res. Pharm., 2014, vol. 3, pp. 154–166.
Cooper, S., Distinguishing between linear and exponential cell growth during the division cycle: single-cell studies, cell-culture studies, and the object of cell-cycle research, Theor. Biol. Med. Model., 2006, vol. 3, p. 10.
Council Directive 86/609/EEC of 24 November 1986 on the approximation of laws, regulations and administrative provisions of the Member States regarding the protection of animals used for experimental and other scientific purposes, Off. J. Eur. Union, 1986, vol. 358, pp. 1–28.
Ding, L. and Morrison S. Haematopoietic stem cells and early lymphoid progenitors occupy distinct bone marrow niches, Nature, 2013, vol. 495, pp. 231–235.
Franco, R., Measurement of red cell lifespan and aging, Med. Hemother., 2012, vol. 39, pp. 302–307.
Gekas, C. and Graf, T., CD41 expression marks myeloid-biased adult hematopoietic stem cells and increases with age, Blood, 2013, vol. 121, pp. 4463–4472.
Glasser, L. and Fiederlein, R., Functional differentiation of normal human neutrophils, Blood, 1987, vol. 69, pp. 937–944.
Ideker, T., Galitski, T., and Hood, L., A new approach to decoding life: systems biology, Ann. Rev. Genomics Hum. Genet., 2001, vol. 2, pp. 343–372.
Kim, J.M., Kim, J., Kim, Y., et al., Comparative secretome analysis of human bone marrow-derived mesenchymal stem cells during osteogenesis, J. Cell. Physiol., 2013, vol. 228, no. 1, pp. 216–224.
Kolaczkowska, E. and Kubes, P., Neutrophil recruitment and function in health and inflammation, Nat. Rev. Immunol., 2013, vol. 13, no. 3, pp. 159–175.
Krause, D., Fulzele, K., Catic, A., et al., Differential regulation of myeloid leukemias by the bone marrow microenvironment, Nat. Med., 2013, vol. 19, pp. 1513–1517.
Krause, D., Theise, N., Collector, M., et al., Multiorgan, multi-lineage engraftment by a single bone marrow- derived stem cell, Cell, 2001, vol. 105, no. 3, pp. 369–377.
Lewandowski, K., Kowalik, M., Pawlaczyk, R., et al., Microscopic examination of bone marrow aspirate in healthy adults—comparison of two techniques of slide preparation, Int. J. Lab. Hematol., 2012, vol. 34, pp. 254–261.
McCracken, J. and Allen, R., Regulation of human neutrophil apoptosis and lifespan in health and disease, J. Cell Death, 2014, vol. 7, pp. 15–23.
Mezey, E., Chandross, K., Harta, G., et al., Turning blood into brain: cells bearing neuronal antigens generated in vivo from bone marrow, Science, 2000, vol. 290, no. 5497, pp. 1779–1782.
Morrison, S. and Scadden, D., The bone marrow niche for haematopoietic stem cells, Nature, 2014, vol. 505, pp. 327–334.
Orlic, D., Kajstura, J., Chimenti, S., et al., Bone marrow cells regenerate infarcted myocardium, Nature, 2000, vol. 410, pp. 701–705.
Pang, W., Price, E., Sahoo, D., et al., Human bone marrow hematopoietic stem cells are increased in frequency and myeloidbiased with age, Proc. Natl. Acad. Sci. U.S.A., 2011, vol. 108, pp. 20012–20017.
Pellicoro, A., Ramachandran, P., Iredale, J., et al., Liver fibrosis and repair: immune regulation of wound healing in a solid organ, Nat. Rev. Immunol., 2014, vol. 14, pp. 181–194.
Rando, T. and Wyss-Coray, T., Stem cells as vehicles for youthful regeneration of aged tissues, J. Gerontol., Ser. A, 2014, vol. 69, pp. 39–42.
Rossi, D., Bryder, D., Zahn, J., et al., Cell intrinsic alterations underlie hematopoietic stem cell aging, Proc. Natl. Acad. Sci. U.S.A., 2005, vol. 102, no. 26, pp. 9194–9199.
Sharpless, N. and DePinho, R., How stem cells age and why this makes us grow old, Nat. Rev. Mol. Cell Biol., 2007, vol. 8, pp. 703–713.
Sudo, K., Ema, H., Morita, Y., et al., Age-associated characteristics of murine hematopoietic stem cells, J. Exp. Med., 2000, vol. 192, no. 9, pp. 1273–1280.
Zhou, S., Greenberger, J., Epperly, M., et al., Agerelated intrinsic changes in human bone marrowderived mesenchymal stem cells and their differentiation to osteoblasts, Aging Cell, 2008, vol. 7, pp. 335–343.
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Bozhkov, A.I., Ohiienko, S.L., Kuznetsova, Y.A. et al. Donor Age Affects Behavior and Sensibility of Bone Marrow Cells to Copper Ions in Primary Culture. Adv Gerontol 7, 336–344 (2017). https://doi.org/10.1134/S2079057017040026
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DOI: https://doi.org/10.1134/S2079057017040026