Abstract
Specific features of the regulation of free-radical oxidation in the blood and synovial fluid in knee osteoarthritis were studied. We examined 46 individuals with an average age of 63.26 ± 9.18 years with primary knee osteoarthritis at stages II–III and 27 almost healthy individuals. The enhanced superoxide eliminating activity of the blood plasma was accompanied by a tendency of an increase in nitrosative stress markers, the nitrites/nitrates. In the erythrocytes we observed an increased level of the secondary product of lipid peroxidation, malone dialdehyde, as well as the activation of the superoxide dismutase and glutathione S-transferase with decreased activity of the glutathione peroxidase and a reduced glutathione content. In knee osteoarthritis, the mononuclear cells of the peripheral blood were characterized by considerable activation of the superoxide dismutase and glutathione S-transferase, moderate activation of the catalase, and slight changes in the glutathione peroxidase activity, together with the enhanced activity of the xanthine oxidoreductase and myeloperoxidase. The redox balance disturbance in the mononuclear cells led, in the long run, to oxidative stress development, which contributed to an increased level of lymphocyte apoptosis.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Arutyunyan, A.V., Dubinina, E.E., and Zybina, N.N., Metody otsenki svobodnoradikal’nogo okisleniya i antioksidantnoi sistemy organizma: Metodicheskie rekomendatsii (Analysis Methods of Free Radical Oxidation and Antioxidant System of Organism: Methodological Recommendations), St. Petersburg: Foliant, 2000.
Golikov, P.P., Oksid azota v klinike neotlozhnykh zabolevanii (Role of Nitrogen Oxide in Clinics of Urgent Diseases), Moscow: Medpraktika, 2004.
Dluzhevskaya, T.S., Pogorelova, T.N., and Afonin, A.A., Activity of NADPH-oxidase in analysis of the states of newborn children, Pediatriya, 1989, no. 3, pp. 44–47.
Korolyuk, M.A., Ivanova, L.I., Maiorova, I.G., and Tokarev, V.E., A method for determination of catalase activity, Lab. Delo, 1988, no. 1, pp. 16–19.
Men’shchikova, E.B., Lankin, V.Z., Zenkov, N.K., et al., Okislitel’nyi stress. Prooksidanty i antioksidanty (Oxidative Stress. Prooxidants and Antioxidants), Moscow: Slovo, 2006.
Pavlova, V.N., Sinovial’naya sreda sustavov (Synovial Medium of the Cartilages), Moscow: Meditsina, 1980.
Saidov, M.Z. and Pinegin, B.V., Spectrophotometric determination of mieloperoxidase in phagocyte cells, Lab. Delo, 1991, no. 3, pp. 56–59.
Sirota, T.V., New approach to investigation of autooxidation of adrenaline and its use for change of superoxide dismutase, Vopr. Med. Khim., 1999, vol. 45, no. 3, pp. 263–272.
Stal’naya, I.D. and Garishvili, T.G., The method of determination of malonic dialdehyde using thiobarbi-turic acid, in Sovremennye metody v biokhimii (Modern Methods Applied in Biochemistry), Orekhovich, V.N., Ed., Moscow: Meditsina, 1977, pp. 66–68.
Kheifits, L.B. and Abalkin, V.A., Separation of human blood corpuscle elements within the gradient of ficollverografin density, Lab. Delo, 1973, vol. 10, pp. 579–581.
Anderson, A.S. and Loeser, R.F., Why is osteoarthritis an age-related disease?, Best Pract. Res. Clin. Rheumatol., 2010, vol. 24, pp. 15–26.
Avis, P.G., Bergel, F., and Bray, R.C., Cellular constituents. The chemistry of xanthine oxidize, J. Chem. Soc., 1955, pp. 1100–1105.
Gautam, N., Das, S., Mahapatra, S.K., et al., Ageassociated oxidative damage in lymphocytes, Oxid. Med. Cell Longevity, 2010, vol. 3, no. 4, pp. 275–282.
Hayes, J.D., Flanagan, J.U., and Jowsey, I.R., Glutathione transferases, Ann. Rev. Pharmacol. Toxicol., 2005, vol. 45, pp. 51–88.
Henrotin, Y., Kurz, B., and Aigner, T., Oxygen and reactive oxygen species in cartilage degradation: friends or foes?, Osteoarthritis Cartilage, 2005, vol. 13, pp. 643–654.
Loeser, R.F., Aging and osteoarthritis: the role of chondrocyte senescence and aging changes in the cartilage matrix, Osteoarthritis Cartilage, 2009, vol. 17, pp. 971–979.
Ostalowska, A., Kasperczyk, S., Kasperczyk, A., et al., Oxidant and anti-oxidant systems of synovial fluid from patients with knee post-traumatic arthritis, J. Orthop. Res., 2007, vol. 25, no. 6, pp. 804–812.
Pamplona, R., Membrane phospholipids, lipoxidative damage, and molecular integrity: a causal role in aging and longevity, Biochim. Biophys. Acta, 2008, vol. 1777, pp. 1249–1262.
Pigeolet, E., Corbisier, P., Houbion, A., et al., Glutathione peroxidase, superoxide dismutase, and catalase inactivation by peroxides and oxygen derived free radicals, Mech. Ageing Dev., 1990, vol. 51, pp. 283–297.
Rajendran, P., Nandakumar, N., Rengarajan, T., et al., Antioxidants and human diseases, Clin. Chim. Acta, 2014, vol. 436, pp. 332–347.
Regan, E.A., Bowler, R.P., and Crapo, J.D., Joint fluid antioxidants are decreased in osteoarthritic joints compared to joints with macroscopically intact cartilage and subacute injury, Osteoarthritis Cartilage, 2008, vol. 16, pp. 515–521.
Scott, J.L., Gabrielides, C., and Davidson, R.K., Superoxide dismutase down regulation in osteoarthritis progression and end-stage disease, Ann. Rheum. Dis., 2010, vol. 69, pp. 1502–1510.
Shah, R., Raska, K., Jr., and Tiku, M.L., The presence of molecular markers of in vivo lipid peroxidation in osteoarthritic cartilage: a pathogenic role in osteoarthritis, Arthritis Rheumatol., 2005, vol. 52, pp. 2799–2807.
Steinbeck, M.J., Nesti, L.J., Sharkey, P.F., and Parvizi, J., Myeloperoxidase and chlorinated peptides in osteoarthritis: potential biomarkers of the disease, J. Orthop. Res., 2007, vol. 25, no. 9, pp. 1128–1135.
Sutipornpalangkul, W., Morales, N.P., Charoencholvanich, K., and Harnroongroj, T., Lipid peroxidation, glutathione, vitamin E, and antioxidant enzymes in synovial fluid from patients with osteoarthritis, Int. J. Rheum. Dis., 2009, vol. 12, no. 4, pp. 324–328.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © V.V. Vnukov, S.B. Panina, I.V. Krolevets, N.P. Milutina, A.A. Ananyan, M.A. Zabrodin, A.A. Plotnikov, 2015, published in Uspekhi Gerontologii, 2015, Vol. 28, No. 2, pp. 284–289.
Rights and permissions
About this article
Cite this article
Vnukov, V.V., Panina, S.B., Krolevets, I.V. et al. Specificities of oxidative stress in the blood and synovial fluid in knee osteoarthritis. Adv Gerontol 5, 261–266 (2015). https://doi.org/10.1134/S2079057015040220
Published:
Issue Date:
DOI: https://doi.org/10.1134/S2079057015040220