Abstract
The cytogenetic and cytotoxic activities of nanosilver particles and silver sulfate in germ cells in vivo were studied in a model similar to the potential impact on humans for the first time. We investigated nanosilver particles with a diameter of 14 nm coated with gum. The substances were ingested by male CBAB6F1 mice for 14 days with drinking water over a wide range of concentrations: 0.1, 50, and 500 mg/L (0.01, 5, and 50 mg/kg). Silver nanoparticles caused a slight statistically significant increase in the frequency of micronucleated spermatids to 0.57‰. Genomic instability affects the cell kinetics: it resulted in an increase in apoptosis from 3.4% (control) to 6.4% (50 mg/L), and it reduced the level of multinucleated spermatids from 15.6% (control) to 11.1% (50 mg/L). This phenomenon can be seen as a compensatory response aimed at death of genetically damaged cells and the expedited renewal of round spermatids by the disintegration of multinucleated spermatids. The minimally tested concentration of silver sulfate increased apoptotic activity. No other changes in the same mode of action of silver sulfate were observed, indicating a more pronounced effect of silver nanoparticles when compared to its ionic form in the germ cells of mice.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
M. M. Dobrzynska, A. Gajowik, J. Radzikowska, A. Lankoff, M. Dušinská, and M. Kruszewski, “Genotoxicity of silver and titanium dioxide nanoparticles in bone marrow cells of rats in vivo,” Toxicology 315, 86 (2014). doi: 10.1016/jtox.2013.11.012.
S. Sinha, A. Chakraborty, S. K. Mallick, M. Bandyopadhyay, and A. Mukherjee, “In vitro and in vivo genotoxicity of silver nanoparticles,” Mutat. Res. 749, 60 (2012).
M. F. Song, Y. S. Li, H. Kasai, and K. Rawai, “Metal nanoparticle-induced micronuclei and oxidative DNA damage in mice,” J. Clin. Biochem. Nutrit. 50, 211 (2012).
Y. Li, J. A. Bhalli, W. Ding, J. Yan, M. G. Pearce, et al., “Cytotoxicity and genotoxicity assessment of silver nanoparticles in mouse,” Nanotoxicology 8 (Suppl. 1), 36 (2014). doi: 10.3109/17435390.2013.855827.
C. G. Ordzhonikidze, L. K. Ramaiyya, E. M. Egorova, and A. V. Rubanovich, “Genotoxic effects of silver nanoparticles on mice in vivo,” Acta Naturae 1 (3), 99 (2009).
T. Colborn and M. J. Smolen, “Epidemiological analysis of persistent organochlorine contaminants in cetaceans,” Rev. Environ. Contam. Toxicol. 146, 91 (1996).
J. Gromadzka-Ostrowska, K. Dziendzikowska, A. Lankoff, M. Dobrzynska, C. Instanes, et al., “Silver nanoparticles effects on epididymal sperm in rats,” Toxicol. Lett. 214, 251 (2012).
Silver sulfate. Safety Data Sheet according to Regulation (EU) No. 1907/2006, Vers. 1.3, Revision Date 02.06.2014. http://wwwmerckmilliporecom/ INTERSHOP/web/WFS/Merck-RU-Site/ru_RU/-/ USD/ProcessMSDS-Start?PlainSKU= MDA_CHEM-101534&lOrigin=SERP
Polyorganic Micronuclear Test in Ecological and Hygienic Studies, Ed. by Yu. A. Rakhmanin and L. P. Sycheva (Genius, Moscow, 2007) [in Russian].
L. P. Sycheva, R. I. Mikhailova, N. N. Belyaeva, V. S.Zhurkov, V. V. Yurchenko, O. N. Savostikova, A. V. Alekseeva, E. K. Krivtsova, M. A. Kovalenko, L. V. Ahaltseva, S. M. Sheremet’eva, N. A. Yurtseva, and L. V. Muravyeva, “Study of mutagenic and cytotoxic effects of multiwalled carbon nanotubes and activated carbon in six organs of mice in vivo,” Nanotechnol. Russ. 10, 311 (2015).
L. P. Sycheva, V. S. Zhurkov, V. V. Iurchenko, N. O. Daugel-Dauge, M. A. Kovalenko, et al., “Investigation of genotoxic and cytotoxic effects of micro-and nanosized titanium dioxide in six organs of mice in vivo,” Mutation Res. 726, 8 (2011).
L. P. Sycheva, “Necessity to evaluate the genetic safety of xenobiotics in mammalian experiments,” Gig. Sanit., No. 5, 18 (2011) [in Russian].
D. Morton, E. Weisbrode, W. E. Wyder, J. K. Maurer, and C. C. Capen, “Spermatid giant cells, tubular hypospermatogenesis, spermatogonial swelling, cytoplasmic vacuoles and tubular dilatation in the testes of normal rabbits,” Vet. Pathol. 23, 176 (1986).
S. K. Singh and K. Abe, “Light and electron microscopic observations of giant cells in the mouse testis after efferent duct ligation,” Arch. Histol. Jpn. 50, 579 (1987).
E. Anton, “Arrested apoptosis without nuclear fragmentation produced by efferent duct ligation in round spermatids and multinucleated giant cells of rat testis,” Reproduction 125, 879 (2003).
M. P. Hande and S. Valiyaveetti, “Anti-proliferative activity of silver nanoparticles,” BMC Cell Biol. 10, 65 (2009).
M. Ahamed, M. Karns, M. Goodson, J. Rowe, S.M. Hussain, et al., “DNA damage response to different surface chemistry of silver nanoparticles in mammalian cells,” Toxicol. Appl. Pharmacol. 233, 404 (2008). doi: 10.1016/jtaap.2008.09.015.
Y. Xue, T. Zhang, B. Zhang, F. Gong, Y. Huang, and M. Tang, “Cytotoxicity and apoptosis induced by silver nanoparticles in human liver HepG2 cells in different dispersion media,” J. Appl. Toxicol. (2015). doi: 10.1002/jat.3199.
G. Kumar, H. Degheidy, B. J. Casey, and P. L. Goering, “Flow cytometry evaluation of in vitro cellular necrosis and apoptosis induced by silver nanoparticles,” Food Chem. Toxicol. (2015). pii: S0278-6915(15)00200-8. doi: 10.1016/jfct.2015.06.012.
L. M. Sosedova, M. A. Novikov, E. A. Titov, and V. S. Rukavishnikov, “Evaluation of biologic effects caused by nano-silver influence on brain tissue of experimental animals,” Med. Truda Promyshl. Ekol., No. 4, 26 (2015).
W. Likus, G. Bajor, and K. Siemianowicz, “Nanosilver—does it have only one face?,” Acta Biochim. Polon. 60, 495 (2013).
Yu. A. Rakhmanin, L. V. Khripach, R. I. Mikhailova, Z. I. Koganova, E. V. Knyazeva, et al., “Comparative analysis of the influence of nano-and ionic forms of silver on biochemical indices in laboratory animals,” Gig. Sanit., No. 1, 45 (2014).
C. Castellini, S. Ruggeri, S. Mattioli, G. Bernardini, L. Macchioni, et al., “Long-term effects of silver nanoparticles on reproductive activity of rabbit buck,” Syst. Biol. Reprod. Med. 60, 143 (2014).
J. H. Lee, Y. S. Kim, K. S. Song, et al., “Biopersistence of silver nanoparticles in tissues from Sprague–Dawley rats,” Part. Fibre Toxicol. 10, 36 (2013).
E. Moretti, G. Terzuoli, T. Renieri, F. Iacoponi, C. Castellini, et al., “In vitro effect of gold and silver nanoparticles on human spermatozoa,” Andrologia 45, 392 (2013). doi: 10.1111/and.12028.
A. R. Gliga, S. Skoglund, I. O. Wallinder, B. Fadeel, and H. L. Karlsson, “Size-dependent cytotoxicity of silver nanoparticles in human lung cells: the role of cellular uptake, agglomeration and Ag release,” Part Fibre Toxicol. 17 (11), 11 (2014). doi: 10.1186/1743-8977-11-11.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © L.P. Sycheva, L.V. Murav’eva, V.S. Zhurkov, R.I. Mikhailova, O.N. Savostikova, A.V. Alekseeva, S.M. Sheremet’eva, 2016, published in Rossiiskie Nanotekhnologii, 2016, Vol. 11, Nos. 3–4.
Rights and permissions
About this article
Cite this article
Sycheva, L.P., Murav’eva, L.V., Zhurkov, V.S. et al. Study of cytogenetic and cytotoxic effects of nanosilver and silver sulfate in germ cells of mice in vivo. Nanotechnol Russia 11, 256–262 (2016). https://doi.org/10.1134/S1995078016020191
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1995078016020191