Abstract
Luminol-dependent chemiluminescence (CL) of blood neutrophils stimulated with phorbol-12- myristate-13-acetate (PMA) and myeloperoxidase (MPO) activity of neutrophils and plasma have been investigated in children (n = 16) during the early period (1−7 days) after thermal skin burns exceeding 20% of total body surface. The CL level of stimulated neutrophils was higher in burn patients than in healthy children of the reference group (p lt; 0.01). Increased neutrophil MPO activity was found in 40% of patients, while increased plasma MPO activity was detected in 57% of patients. The albumin fraction isolated from plasma of burn patients increased the PMA-stimulated CL response of blood from healthy donors. These results suggest that the acute inflammatory response to the thermal burn causes neutrophil activation and MPO release into plasma. MPO-mediated modification of plasma proteins, particularly albumin, may stimulate neutrophil activation and provoke further inflammatory response of the body to the thermal injury.
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Original Russian Text © E.V. Mikhalchik, L.I. Budkevich, Yu.A. Piterskaya, L.Yu. Penkov, T.S. Astamirova, N.V. Smolina, T.V. Vakhrusheva, O.M. Panasenko, 2017, published in Biomeditsinskaya Khimiya.
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Mikhalchik, E.V., Budkevich, L.I., Piterskaya, Y.A. et al. The role of neutrophil myeloperoxidase in the development of inflammation induced by thermal skin burns. Biochem. Moscow Suppl. Ser. B 11, 72–75 (2017). https://doi.org/10.1134/S1990750817010073
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DOI: https://doi.org/10.1134/S1990750817010073