Abstract
The radical-producing activity of human platelets has been studied using the enhanced chemiluminescence method. It is shown that chemiluminescence of isolated platelets is observed only in the presence of lucigenin, a selective probe for superoxide anion; the luminescence is amplified many times upon the addition of NADH and NADPH, the substrates of oxidative chains. The chemiluminescence is not affected by diphenyliodonium, an inhibitor of NADPH oxidase, but it is inhibited in a dose-dependent manner by the oxidative phosphorylation uncouplers dinitrophenol and rotenone. Thus, a superoxide anion radical is the main free radical generated by platelets, and mitochondria are one of the superoxide anion radical sources in platelets.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Panday A., Sahoo M.K., Osorio D., Batra S. 2015. NADPH oxidases: An overview from structure to innate immunity-associated pathologies. Cell Mol. Immunol. 12, 5–23.
Kozjak-Pavlovic V. 2017. The MICOS complex of human mitochondria. Cell Tissue Res. 367 (1), 83–93.
Bae Y.S., Oh H., Rhee S.G., Yoo Y.D. 2011. Regulation of reactive oxygen species generation in cell signaling. Mol. Cells. 32, 491–509.
Labat-Robert J., Robert L. 2014. Longevity and aging. Role of free radicals and xanthine oxidase. A review. Pathol. Biol. (Paris). 62, 61–66.
Gu Y., Xu Y., Law B., Qian S.Y. 2013. The first characterization of free radicals formed from cellular COX-catalyzed peroxidation. Free Radic. Biol. Med. 57, 49–60.
Pratico D., Pasin M., Barry O.P., Ghiselli A., Sabatino G., Iuliano L., FitzGerald G.A., Violi F. 1999. Iron-dependent human platelet activation and hydroxyl radical formation: Involvement of protein kinase C. Circulation. 99, 3118–3124.
Caccese D., Pratico D., Ghiselli A., Natoli S., Pignatelli P., Sanguigni V., Iuliano L., Violi F. 2000. Superoxide anion and hydroxyl radical release by collagen-induced platelet aggregation—role of arachidonic acid metabolism. Thromb. Haemost. 83, 485–490.
Ferroni P., Vazzana N., Riondino S., Cuccurullo C., Guadagni F., Davi G. 2012. Superoxide anion and hydroxyl radical release by collagen-induced platelet aggregation—role of arachidonic acid metabolism. Thromb. Haemos.t 83, 485–490.
Finazzi-Agro A., Menichelli A., Persiani M., Biancini G., Del Principe D. 1982. Hydrogen peroxide release from human blood platelets. Biochim. Biophys. Acta. 718, 21–25.
Loscalzo J. 1985. N-Acetylcysteine potentiates inhibition of platelet aggregation by nitroglycerin. J. Clin. Invest. 76, 703–708.
Wachowicz B., Olas B., Zbikowska H.M., Buczynski A. 2002. Generation of reactive oxygen species in blood platelets. Platelets. 13, 175–182.
Krotz F., Sohn H.Y., Pohl U. 2004. Reactive oxygen species: Players in the platelet game. Arterioscler. Thromb. Vasc. Bio.l 24, 1988–1996.
Krotz F., Sohn H.Y., Gloe T., Zahler S., Riexinger T., Schiele T.M., Becker B.F., Theisen K., Klauss V., Pohl U. 2002. NAD(P)H oxidase-dependent platelet superoxide anion release increases platelet recruitment. Blood. 100, 917–924.
McVeigh G.E., Hamilton P., Wilson M., Hanratty C.G., Leahey W.J., Devine A.B., Morgan D.G., Dixon L.J., McGrath L.T. 2002. Platelet nitric oxide and superoxide release during the development of nitrate tolerance: Effect of supplemental ascorbate. Circulation. 106, 208–213.
Lassegue B., Clempus R.E. 2003. Vascular NAD(P)H oxidases: Specific features, expression, and regulation. Am. J. Physiol. Regul. Integr. Comp. Physiol. 285, R277–297.
Begonja A.J., Gambaryan S., Geiger J., Aktas B., Pozgajova M., Nieswandt B., Walter U. 2005. Platelet NAD(P)H-oxidase-generated ROS production regulates alphaIIbbeta3-integrin activation independent of the NO/cGMP pathway. Blood. 106, 2757–2760.
Chlopicki S., Olszanecki R., Janiszewski M., Laurindo F.R., Panz T., Miedzobrodzki J. 2004. Functional role of NADPH oxidase in activation of platelets. Antioxid. Redox Signal. 6, 691–698.
Zalavary S., Grenegard M., Stendahl O., Bengtsson T. 1996. Platelets enhance Fc(gamma) receptor-mediated phagocytosis and respiratory burst in neutrophils: The role of purinergic modulation and actin polymerization. J. Leukoc. Biol. 60, 58–68.
FitzGerald G.A. 2003. COX-2 and beyond: Approaches to prostaglandin inhibition in human disease. Nat. Rev. Drug Discov. 2, 879–890.
Niwa K., Haensel C., Ross M.E., Iadecola C. 2001. Cyclooxygenase-1 participates in selected vasodilator responses of the cerebral circulation. Circ. Res. 88, 600–608.
Jahn B., Hansch G.M. 1990. Oxygen radical generation in human platelets: Dependence on 12-lipoxygenase activity and on the glutathione cycle. Int. Arch. Allergy Appl. Immunol. 93, 7-3-79.
Singh D., Greenwald J.E., Bianchine J., Metz E.N., Sagone A.L., Jr. 1981. Evidence for the generation of hydroxyl radical during arachidonic acid metabolism by human platelets. Am. J. Hematol. 11, 233–240.
Gilbert G.E., Sims P.J., Wiedmer T., Furie B., Furie B.C., Shattil S.J. 1991. Platelet-derived microparticles express high affinity receptors for factor VIII. J. Biol. Chem. 266, 17261–17268.
Storrie B., Madden E.A. 1990. Isolation of subcellular organelles. Meth. Enzymol. 182, 203–225.
Jezek P., Hlavata L. 2005. Mitochondria in homeostasis of reactive oxygen species in cell, tissues, and organism. Int. J. Biochem. Cell. Biol. 37, 2478–2503.
Sottocasa G.L., Kuylenstierna B., Ernster L., Bergstrand A. 1967. An electron-transport system associated with the outer membrane of liver mitochondria. J. Cell. Biol. 32, 415–438.
Archakov A.I. 1975. Mikrosomal’noe okislenie (Microsomal oxidation). M.: Nauka.
Aasen T.B., Bolann B., Glette J., Ulvik R.J., Schreiner A. 1987. Lucigenin-dependent chemiluminescence in mononuclear phagocytes. Role of superoxide anion. Scand. J. Clin. Lab. Invest. 47, 673–679.
Kruglov A.G., Yurkov I.S., Teplova V.V., Evtodienko Y.V. 2002. Lucigenin-derived chemiluminescence in intact isolated mitochondria. Biochemistry (Moscow). 67, 1262–1270.
Lu H., Burns D., Garnier P., Wei G., Zhu K., Ying W. 2007. P2X7 receptors mediate NADH transport across the plasma membranes of astrocytes. Biochem. Biophys. Res. Commun. 362, 946–950.
Dietrich-Muszalska A., Kwiatkowska A. 2014. Generation of superoxide anion radicals and platelet glutathione peroxidase activity in patients with schizophrenia. Neuropsychiatric Dis. Treat. 10, 703–709.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A.A. Dzhatdoeva, E.V. Proskurnina, A.M. Nesterova, I.V. Dubinkin, T.V. Gaponova, S.I. Obydenny, Yu.A. Vladimirov, 2017, published in Biologicheskie Membrany, 2017, Vol. 34, No. 6, pp. 116–123.
Rights and permissions
About this article
Cite this article
Dzhatdoeva, A.A., Proskurnina, E.V., Nesterova, A.M. et al. Mitochondria as a Source of Superoxide Anion Radical in Human Platelets. Biochem. Moscow Suppl. Ser. A 12, 43–49 (2018). https://doi.org/10.1134/S1990747818010051
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1990747818010051