Abstract
A new high-precision technique for calculating the ratio of the erythrocyte area/volume using atomic-force microscopy has been developed. The method was tested on erythrocytes of healthy donors. Scanning electron microscopy, atomic-force microscopy, and X-ray microanalysis revealed that magnetite nanoparticles can interact with erythrocyte membranes in vitro. This interaction resulted in the development of a pathology of erythrocytes typical for poikilocytosis and anisocytosis. When the magnetite was incubated with erythrocytes in a serum-free medium, nanoparticles aggregated.
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Abbreviations
- AFM:
-
atomic-force microscopy
- MNPs:
-
magnetite nanoparticles
- SEM:
-
scanning electron microscopy
- PBS:
-
phosphate buffer saline
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Original Russian Text © S.N. Pleskova, E.E. Gornostaeva, R.N. Kryukov, A.V. Boryakov, S.Yu. Zubkov, 2017, published in Tsitologiya, 2017, Vol. 59, No. 12, pp. 874–880.
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Pleskova, S.N., Gornostaeva, E.E., Kryukov, R.N. et al. Changes in the Architectonics and Morphometric Characteristics of Erythocytes under the Influence of Magnetite Nanoparticles. Cell Tiss. Biol. 12, 127–134 (2018). https://doi.org/10.1134/S1990519X18020086
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DOI: https://doi.org/10.1134/S1990519X18020086