Abstract
Interactions between bone marrow stromal cells (BMSCs) and cartilage cells were studied in cell cocultures. Actin cytoskeleton organization and the cell spreading on various extracellular matrix proteins (laminin 2/4, collagen type I, and fibronectin) were explored. It was found that the most pronounced morphological changes (cell shape and area, actin cytoskeleton organization) were observed in cells cultivated on fibronectin. The average spreading area of BMSCs grown on fibronectin was about four times larger than the spreading area of cartilage cells. In cocultures of these cells plated in a ratio of 1: 1, the cell spreading area on fibronectin proved to be 1.5 times less than was theoretically calculated. To clarify what influence cells have on each other, cell spreading in the conditioned medium was assayed. It was round that the BMSC spreading area in a cartilage cell conditioned medium was significantly less than in the control serum-free medium. This shows that cartilage cells are the source of factors that affect BMSC spreading.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- ECM:
-
extracellular matrix
- BMSC:
-
bone marrow stromal cell
References
Afanasjev, Yu.I. and Omelyanenko, N.P., Connective tissues, in Rukovodstvo po gistologii (Histology Manual), St. Petersburg: SpetsLit, 2001, vol. 1, pp. 249–283.
Are, A., Pinaev, G., Burova, E., and Lindberg, U., Attachment of A-431 cells on immobilized antibodies to the EGF receptor promotes cell spreading and reorganization of the microfilament system, Cell Motil. Cytoskeleton, 2001, vol. 48, pp. 24–36.
Are, A.F., Pospelova, T.V., and Pinaev, G.P., The characteristics of actin cytoskeleton structure and its rearrangements by extracellular matrix proteins in normal, immortalized and transformed rat fibroblasts, Tsitologiia, 1999, vol. 41, no. 8, pp. 707–715.
Brittberg, M., Peterson, L., Sjogren-Jansson, E., Tallheden, T., and Lindahl, A., Articular cartilage engineering with autologous chondrocyte transplantation. A review of recent developments, Bone Joint Surg. Am., 2003, vol. 85-A, pp. 109–115.
Chang, N.J., Lam, C.F., Lin, C.C., Chen, W.L., Li, C.F., Lin, Y.T., and Yeh, M.L., Transplantation of autologous endothelial progenitor cells in porous PLGA scaffolds create a microenvironment for the regeneration of hyaline cartilage in rabbits, Osteoarthritis Cartilage, 2013, vol. 21, pp. 1613–1622.
Dai, W., Kawazoe, N., Lin, X., Dong, J., and Chen, G., The influence of structural design of PLGA/collagen hybrid scaffolds in cartilage tissue engineering, Biomaterials, 2010, vol. 31, pp. 2141–2152.
Ermakova, I.I., Chertkova, T.A., Mokrushin, A.L., Romaniouk, A.V., Sakuta, G.A., and Morozov, V.I., Proteoglycans of L6J1 myoblast extracellular matrix. Characteristics and effect on myoblast adhesion, Tsitologiia, 2008, vol. 50, no. 8, pp. 692–699.
Galle, J., Bader, A., Hepp, P., Grill, W., Fuchs, B., Käs, J.A., Krinner, A., Marquass, B., Müller, K., Schiller, J., Schulz, R.M., von Buttlar, M., von der Burg, E., Zscharnack, M., and Löffler, M., Mesenchymal stem cells in cartilage repair: state of the art and methods to monitor cell growth, differentiation and cartilage regeneration, Curr. Med. Chem., 2010, vol. 17, pp. 2274–2291.
Ganey, T., Hutton, W.C., Moseley, T., Hedrick, M., and Meisel, H.J., Intervertebral disc repair using adipose tissuederived stem and regenerative cells: experiments in a canine model, Spine, 2009, vol. 34, pp. 2297–2304.
Hall, B.K., Cartilage, Vol. 1: Structure, Function, and Biochemistry, New York: Academic Press, 1983.
Han, Y.L., Wang, S., Zhang, X., Li, Y., Huang, G., Qi, H., Pingguan-Murphy, B., Lu, T.J., Xu, F., Engineering physical microenvironment for stem cell based regenerative medicine, Drug Discov. Today, 2014, vols. 1359–6446, pp. 00033–00036.
Hui, J.H.P., Azura, M., and Lee, E.H., Review article: stem cell therapy in orthopaedic surgery: current status and ethical considerations, Malaysian Orthopaedic J., 2009, vol. 3, pp. 4–12.
Imoto, E., Kakuta, S., Hori, M., Yagami, K., and Nagumo, M., Adhesion of a chondrocytic cell line (USAC) to fibronectin and its regulation by proteoglycan, Oral Pathol. Med., 2002, vol. 31, pp. 35–44.
Khubutiya, M.Sh., Kliukvin, I.Y., Istranov, L.P., Khvatov, V.B., Shekhter, A.B., Vaza, A.Y., Kanakov, I.V., and Bocharova, V.S., Stimulation of regeneration of hyaline cartilage in experimental osteochondral injury, Bull. Exp. Biol. Med., 2008, vol. 146, pp. 658–661.
Koga, H., Muneta, T., Ju, Y.-J., Nagase, T., Nimura, A., Mochizuki, T., Ichinose, S., von der Mark, K., and Sekiya, I., Synovial stem cells are regionally specified according to local microenvironments after implantation for cartilage regeneration, Stem Cells, 2007, vol. 25, pp. 689–696.
Kopesky, P.W., Lee, H.Y., Vanderploeg, E.J., Kisiday, J.D., Frisbie, D.D., Plaas, A.H., Ortiz, C., and Grodzinsky, A.J., Adult equine bone marrow stromal cells produce a cartilage-like ECM mechanically superior to animal-matched adult chondrocytes, Matrix Biol., 2010, vol. 29, pp. 427–438.
Pörtner, R. and Meenen, N.M., Technological aspects of regenerative medicine and tissue engineering of articular cartilage, Handchir. Mikrochir. Plast. Chir., 2010, vol. 42, pp. 329–336.
Palm, S.L. and Furcht, L.T., Production of laminin and fibronectin by schwannoma cells: cell-protein interactions in vitro and protein localization in peripheral nerve in vivo, Cell Biol., 1983, vol. 96, pp. 1218–1266.
Parsons, P., Gilbert, S.J., Vaughan-Thomas, A., Sorrell, D.A., Notman, R., Bishop, M., Hayes, A.J., Mason, D.J., and Duance, V.C., Type IX collagen interacts with fibronectin providing an important molecular bridge in articular cartilage, Biol. Chem., 2011, vol. 286, pp. 34986–34997.
Petukhova, O.A., Turoverova, L.V., Kropacheva, I.V., and Pinaev, G.P., Morphological peculiarities of epidermoid carcinoma A431 cells spread on immobilized ligands, Tsitologiia, 2004, vol. 46, no. 1, pp. 5–15.
Richardson, S.M., Hoyland, J.A., and Mobasheri, R., Mesenchymal stem cells in regenerative medicine: opportunities and challenges for articular cartilage and intervertebral disc tissue engineering, Cell. Physiol., 2010, vol. 222, pp. 23–32.
Tobita, M., Ochi, M., Uchio, Y., Mori, R., Iwasa, J., Katsube, K., and Motomura, T., Treatment of growth plate injury with autogenous chondrocytes, Acta Orthop. Scand., 2002, vol. 73, pp. 352–358.
Yoshimura, H., Muneta, T., Nimura, A., Yokoyama, A., Koga, H., and Sekiya, I., Comparison of rat mesenchymal stem cells derived from bone marrow, synovium, periosteum, adipose tissue, and muscle, Cell Tissue Res., 2007, vol. 327, pp. 449–462.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © E.I. Sachenberg, N.N. Nikolaenko, G.P. Pinaev, 2014, published in Tsitologiya, 2014, Vol. 56, No. 10, pp. 708–716.
Rights and permissions
About this article
Cite this article
Sachenberg, E.I., Nikolaenko, N.N. & Pinaev, G.P. Spreading and actin cytoskeleton organization of cartilage and bone marrow stromal cells cocultured on various extracellular matrix proteins. Cell Tiss. Biol. 9, 1–8 (2015). https://doi.org/10.1134/S1990519X15010083
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1990519X15010083